Any feedback?
Literature summary for 2.7.1.59 extracted from
- Structure and function of N-acetylglucosamine kinase. Identification of two active site cysteines (2002), Eur. J. Biochem., 269, 4212-4218.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| 6 point mutations in cysteine residues, expression in Escherichia coli BL21 | Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C131S | strongly decreased activity, very low affinity for N-acetyl-D-glucosamine | Mus musculus |
| C143S | strongly decreased activity, very low affinity for ATP | Mus musculus |
| C211S | slightly decreased activity | Mus musculus |
| C217S | decreased activity, low affinity for N-acetyl-D-glucosamine | Mus musculus |
| C268S | decreased activity | Mus musculus |
| C45S | very slightly decreased activity, no function in substrate binding | Mus musculus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km for both substrates tested for wild type and all mutant enzymes | Mus musculus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39000 | - |
SDS-PAGE, wild type protein | Mus musculus |
| 42000 | - |
SDS-PAGE, from E. coli | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + N-acetyl-D-glucosamine | Mus musculus | first enzyme of N-acetyl-D-glucosamine salvage pathway | ADP + N-acetyl-D-glucosamine 6-phosphate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from E. coli | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + N-acetyl-D-glucosamine | - |
Mus musculus | ADP + N-acetyl-D-glucosamine 6-phosphate | - |
? | |
| ATP + N-acetyl-D-glucosamine | first enzyme of N-acetyl-D-glucosamine salvage pathway | Mus musculus | ADP + N-acetyl-D-glucosamine 6-phosphate | - |
? | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
