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Literature summary for 2.7.1.59 extracted from

  • Lee, H.; Dutta, S.; Moon, I.S.
    Upregulation of dendritic arborization by N-acetyl-D-glucosamine kinase is not dependent on its kinase activity (2014), Mol. Cells, 37, 322-329.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3)-pLysS cells Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
C143S the mutant retains 75% of wild type enzymatic activity Rattus norvegicus
D107A inactive Rattus norvegicus
N36A the mutation reduces enzyme activity to 51% of the wild type Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
ATP wild type enzyme, at pH 7.5 and 37°C Rattus norvegicus
0.15
-
ATP mutant enzyme C143S, at pH 7.5 and 37°C Rattus norvegicus
0.78
-
N-acetyl-D-glucosamine wild type enzyme, at pH 7.5 and 37°C Rattus norvegicus
0.86
-
N-acetyl-D-glucosamine mutant enzyme C143S, at pH 7.5 and 37°C Rattus norvegicus
1.17
-
N-acetyl-D-glucosamine mutant enzyme N36A, at pH 7.5 and 37°C Rattus norvegicus
1.59
-
ATP mutant enzyme N36A, at pH 7.5 and 37°C Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
x * 40000, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + N-acetyl-D-glucosamine Rattus norvegicus
-
ADP + N-acetyl-D-glucosamine 6-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
hippocampus
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + N-acetyl-D-glucosamine
-
Rattus norvegicus ADP + N-acetyl-D-glucosamine 6-phosphate
-
?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Rattus norvegicus

Synonyms

Synonyms Comment Organism
GlcNAc kinase
-
Rattus norvegicus
N-acetyl-D-glucosamine kinase
-
Rattus norvegicus
NagK
-
Rattus norvegicus

General Information

General Information Comment Organism
physiological function the small domain of the enzyme, and not its kinase activity, plays a critical role in the upregulation of dendritogenesis Rattus norvegicus