2.7.1.35: pyridoxal kinase
This is an abbreviated version!
For detailed information about pyridoxal kinase, go to the full flat file.
Word Map on EC 2.7.1.35
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2.7.1.35
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5'-phosphate
-
vitamers
-
pyridoxal-5'-phosphate
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plp-dependent
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4-pyridoxic
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ribokinase
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agriculture
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synthesis
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drug development
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medicine
- 2.7.1.35
- 5'-phosphate
-
vitamers
- pyridoxal-5'-phosphate
-
plp-dependent
-
4-pyridoxic
- ribokinase
- agriculture
- synthesis
- drug development
- medicine
Reaction
Synonyms
ePL kinase, ePL kinase 1, hPL kinase, HPLK, kinase (phosphorylating), pyridoxal, kinase, pyridoxal (phosphorylating), LdPdxK, PdxK, pdxY, PKH, PL kinase, PLK, Plk1, PM kinase, PN kinase, PN/PL/PM kinase, pyridoxal 5-phosphate-kinase, pyridoxal kinase, pyridoxal kinase 1, pyridoxal kinase PKL, pyridoxal kinase-like protein SOS4, pyridoxal phosphokinase, pyridoxal/pyridoxine kinase, pyridoxamine kinase, pyridoxine kinase, pyridoxine/pyridoxal/pyridoxamine kinase, salt overly sensitive4, SAV0580, Sos4, StPLK, vitamin B6 kinase
ECTree
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Purification
Purification on EC 2.7.1.35 - pyridoxal kinase
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Ni-NTA agarose column chromatography
Ni-NTA agarose column chromatography and HiLoad 26/60 Superdex 200 gel filtration
Phenyl ToyoPearl 650S column chromatography and Hi-Load Superdex 200 column gel filtration
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recombinant enzyme
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) Codon Plus RIL by nickel affinity chromatography and gel filtration
recombinant His-tagged enzyme from Escherichia coli strain Rosetta 2 (DE3) by nickel affinity chromatography and gel filtration
TMAE column chromatography, Phenyl Sepharose column chromatography, and hydroxyapatite column chromatography