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Literature summary for 2.7.1.35 extracted from

  • Kaestner, U.; Hallmen, C.; Wiese, M.; Leistner, E.; Drewke, C.
    The human pyridoxal kinase, a plausible target for ginkgotoxin from Ginkgo biloba (2007), FEBS J., 274, 1036-1045.
    View publication on PubMed
Search for more literature for 2.7.1.35

Application

Application Comment Organism
medicine ingestion of 4'-O-methylpyridoxine, i.e. ginkgotoxin, triggers epileptic convulsions and other neuronal symptoms. 4'-O-Methylpyridoxine is an competitive inhibitor of pyridoxal kinase and leads to temporarily reduced pyridoxal phosphate formation in vitro and possibly in vivo Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
-
 Homo sapiens
expressed in Escherichia coli strain BL21 (DE3) Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
4'-deoxypyridoxine
-
 Homo sapiens
4'-O-methylpyridoxine i.e. ginkgotoxin. Treatment leads to temporarily reduced pyridoxal phosphate formation in vitro and possibly in vivo Homo sapiens
ginkgotoxin
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0028
-
 pyridoxal in 70 mM potassium phosphate buffer, pH 6.2 Homo sapiens
0.00495
-
 4'-O-methylpyridoxine pH 6.2, 37°C Homo sapiens
0.00495
-
 ginkgotoxin in 70 mM potassium phosphate buffer, pH 6.2 Homo sapiens
0.00987
-
 pyridoxine pH 6.2, 37°C Homo sapiens
0.00987
-
 pyridoxine in 70 mM potassium phosphate buffer, pH 6.2 Homo sapiens
0.0216
-
 4'-deoxypyridoxine pH 6.2, 37°C Homo sapiens
0.0216
-
 4'-deoxypyridoxine in 70 mM potassium phosphate buffer, pH 6.2 Homo sapiens
0.0587
-
 pyridoxal pH 6.2, 37°C Homo sapiens
0.126
-
 pyridoxamine pH 6.2, 37°C Homo sapiens
0.126
-
 pyridoxamine in 70 mM potassium phosphate buffer, pH 6.2 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens O00764
-
-

Purification (Commentary)

Purification (Comment) Organism
pyridoxyl-EAH-Sepharose 4B column chromatography Homo sapiens
recombinant enzyme Homo sapiens

Storage Stability

Storage Stability Organism
-20°C, recombinant enzyme, stable for 21 days Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 4'-deoxypyridoxine
-
 Homo sapiens ADP + 4'-deoxypyridoxine 5'-phosphate
-
 ?
ATP + 4'-O-methylpyridoxine
-
 Homo sapiens ADP + 4'-O-methylpyridoxine 5'-phosphate
-
 ?
ATP + ginkgotoxin
-
 Homo sapiens ADP + ginkgotoxin 5'-phosphate
-
 ?
ATP + pyridoxal
-
 Homo sapiens ADP + pyridoxal 5'-phosphate
-
 ?
ATP + pyridoxal no substrate: GTP Homo sapiens ADP + pyridoxal 5'-phosphate
-
 ?
ATP + pyridoxamine
-
 Homo sapiens ADP + pyridoxamine 5'-phosphate
-
 ?
ATP + pyridoxine
-
 Homo sapiens ADP + pyridoxine 5'-phosphate
-
 ?
additional information no activity with GTP Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
PKH
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-20
-
 stable at -20°C for approximately 21 days Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.46
-
 ginkgotoxin in 70 mM potassium phosphate buffer, pH 6.2 Homo sapiens
0.46
-
 4'-O-methylpyridoxine pH 6.2, 37°C Homo sapiens
0.79
-
 pyridoxamine in 70 mM potassium phosphate buffer, pH 6.2 Homo sapiens
0.79
-
 pyridoxamine pH 6.2, 37°C Homo sapiens
0.995
-
 pyridoxal pH 6.2, 37°C Homo sapiens
1.233
-
 pyridoxine pH 6.2, 37°C Homo sapiens
1.535
-
 4'-deoxypyridoxine pH 6.2, 37°C Homo sapiens
2.94
-
 4'-deoxypyridoxine in 70 mM potassium phosphate buffer, pH 6.2 Homo sapiens
6.08
-
 pyridoxal in 70 mM potassium phosphate buffer, pH 6.2 Homo sapiens
6.08
-
 pyridoxine in 70 mM potassium phosphate buffer, pH 6.2 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8 6.3
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000414
-
 ginkgotoxin
-
 Homo sapiens
0.000414
-
 4'-O-methylpyridoxine pH 6.2, 37°C Homo sapiens
0.0574
-
 4'-deoxypyridoxine
-
 Homo sapiens
0.0574
-
 4'-deoxypyridoxine pH 6.2, 37°C Homo sapiens