Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 0.1 M Tris, pH 8.5, 1 M LiCl2, and 15% (w/v) PEG 6000 | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
C124A | the mutant shows about 2fold increased catalytic efficiency compared to the wild type enzyme | Pseudomonas aeruginosa |
D225A | inactive | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.037 | - |
pyridoxal | mutant enzyme C124A, at pH 7.4 and 37°C | Pseudomonas aeruginosa | |
0.053 | - |
pyridoxal | wild type enzyme, at pH 7.4 and 37°C | Pseudomonas aeruginosa | |
0.078 | - |
ATP | mutant enzyme C124A, at pH 7.4 and 37°C | Pseudomonas aeruginosa | |
0.102 | - |
ATP | wild type enzyme, at pH 7.4 and 37°C | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyridoxal | Pseudomonas aeruginosa | - |
ADP + pyridoxal 5'-phosphate | - |
? | |
ATP + pyridoxal | Pseudomonas aeruginosa PAO | - |
ADP + pyridoxal 5'-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Pseudomonas aeruginosa PAO | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Mono Q column chromatography | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyridoxal | - |
Pseudomonas aeruginosa | ADP + pyridoxal 5'-phosphate | - |
? | |
ATP + pyridoxal | - |
Pseudomonas aeruginosa PAO | ADP + pyridoxal 5'-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
PdxK | - |
Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
pyridoxal | wild type enzyme, at pH 7.4 and 37°C | Pseudomonas aeruginosa | |
1.8 | - |
ATP | mutant enzyme C124A, at pH 7.4 and 37°C | Pseudomonas aeruginosa | |
3.3 | - |
ATP | wild type enzyme, at pH 7.4 and 37°C | Pseudomonas aeruginosa | |
4.7 | - |
pyridoxal | mutant enzyme C124A, at pH 7.4 and 37°C | Pseudomonas aeruginosa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.7 | - |
ATP | wild type enzyme, at pH 7.4 and 37°C | Pseudomonas aeruginosa | |
23.1 | - |
ATP | mutant enzyme C124A, at pH 7.4 and 37°C | Pseudomonas aeruginosa | |
62.3 | - |
pyridoxal | wild type enzyme, at pH 7.4 and 37°C | Pseudomonas aeruginosa | |
127 | - |
pyridoxal | mutant enzyme C124A, at pH 7.4 and 37°C | Pseudomonas aeruginosa |