Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.35 extracted from

  • Kimura, T.; Shirakawa, R.; Yaoita, N.; Hayashi, T.; Nagano, K.; Horiuchi, H.
    The antimalarial drugs chloroquine and primaquine inhibit pyridoxal kinase, an essential enzyme for vitamin B6 production (2014), FEBS Lett., 588, 3673-3676.
    View publication on PubMed
Search for more literature for 2.7.1.35

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Chloroquine about 40% inhibition at 1 mM Plasmodium vivax
additional information not inhibited by chloroquine Homo sapiens
additional information not inhibited by chloroquine Trypanosoma cruzi
primaquine competitive inhibition, complete inhibition at 0.75 mM Homo sapiens
primaquine competitive inhibition, complete inhibition at 1 mM Plasmodium vivax
primaquine competitive inhibition, about 70% inhibition at 1 mM Trypanosoma cruzi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00122
-
 pyridoxal in the presence of K+, at pH 7.0 and 30°C Homo sapiens
0.0153
-
 pyridoxal in the presence of K+, at pH 7.0 and 30°C Plasmodium vivax
0.0216
-
 pyridoxal in the presence of K+, at pH 7.0 and 30°C Trypanosoma cruzi
0.0739
-
 pyridoxal in the presence of Na+, at pH 7.0 and 30°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
K+ activates Homo sapiens
K+ activates Trypanosoma cruzi
K+ activates Plasmodium vivax
Na+ activates Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 x * 35000, SDS-PAGE Homo sapiens
35000
-
 x * 35000, SDS-PAGE Trypanosoma cruzi
35000
-
 x * 35000, SDS-PAGE Plasmodium vivax

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + pyridoxal Homo sapiens
-
 ADP + pyridoxal 5'-phosphate
-
 ?
ATP + pyridoxal Trypanosoma cruzi
-
 ADP + pyridoxal 5'-phosphate
-
 ?
ATP + pyridoxal Plasmodium vivax
-
 ADP + pyridoxal 5'-phosphate
-
 ?
ATP + pyridoxal Plasmodium vivax Salvador I
-
 ADP + pyridoxal 5'-phosphate
-
 ?
ATP + pyridoxal Trypanosoma cruzi CL Brener
-
 ADP + pyridoxal 5'-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Plasmodium vivax A5K1Z8
-
-
Plasmodium vivax Salvador I A5K1Z8
-
-
Trypanosoma cruzi Q4D946
-
-
Trypanosoma cruzi CL Brener Q4D946
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Homo sapiens
Ni-NTA agarose column chromatography Trypanosoma cruzi
Ni-NTA agarose column chromatography Plasmodium vivax

Source Tissue

Source Tissue Comment Organism Textmining
lung
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + pyridoxal
-
 Homo sapiens ADP + pyridoxal 5'-phosphate
-
 ?
ATP + pyridoxal
-
 Trypanosoma cruzi ADP + pyridoxal 5'-phosphate
-
 ?
ATP + pyridoxal
-
 Plasmodium vivax ADP + pyridoxal 5'-phosphate
-
 ?
ATP + pyridoxal
-
 Plasmodium vivax Salvador I ADP + pyridoxal 5'-phosphate
-
 ?
ATP + pyridoxal
-
 Trypanosoma cruzi CL Brener ADP + pyridoxal 5'-phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 35000, SDS-PAGE Homo sapiens
? x * 35000, SDS-PAGE Trypanosoma cruzi
? x * 35000, SDS-PAGE Plasmodium vivax

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.015
-
 pyridoxal in the presence of K+, at pH 7.0 and 30°C Trypanosoma cruzi
0.073
-
 pyridoxal in the presence of K+, at pH 7.0 and 30°C Plasmodium vivax
0.12
-
 pyridoxal in the presence of K+, at pH 7.0 and 30°C Homo sapiens
0.23
-
 pyridoxal in the presence of Na+, at pH 7.0 and 30°C Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0572
-
 primaquine at pH 7.0 and 30°C Homo sapiens