Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta (DE3)pLysS cells | Homo sapiens |
expression in Escherichia coli | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method using 100 mM Tris-HCl pH 8.0 and 50% 2-methyl-2,4-pentanediol | Homo sapiens |
unliganded enzyme and in complex with MgATP, diffraction to 2.0 and 2.2 A rsolution, respectively. both Structures show similar open conformations. Mg2+ and Na+ act in tandem to anchor the ATP at the active site, which itself acts as a sink to bind several molecules of 2-methyl-2,4-pentanediol | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Na+ | inhibits above 50 mM | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
pyridoxal | in the presence of K+ | Homo sapiens | |
0.01 | - |
pyridoxal | presence of 40 mM Na+, pH 7.3, 37°C | Homo sapiens | |
0.025 | - |
ATP | in the presence of K+, and in complex with Mg2+ | Homo sapiens | |
0.025 | - |
ATP | presence of 40 mM Na+, pH 7.3, 37°C | Homo sapiens | |
0.075 | - |
pyridoxal | in the presence of Na+ | Homo sapiens | |
0.075 | - |
pyridoxal | presence of 40 mM Na+, pH 7.3, 37°C | Homo sapiens | |
0.5 | - |
ATP | in the presence of Na+, and in complex with Mg2+ | Homo sapiens | |
0.5 | - |
ATP | presence of 40 mM Na+, pH 7.3, 37°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | affinity to pyridoxal and ATP is increased manifold in the presence of K+ compared to Na+, 2.5fold increase of activity | Homo sapiens | |
K+ | activation. Affinity for ATP and pyridoxal is increased severalfold in presence of K+ compared with Na+, but the maximal activity of the Na+ form is more than double the activity of the K+ form | Homo sapiens | |
Mg2+ | acts in complex with ATP | Homo sapiens | |
additional information | Li+, Cs+, and Rb+ show no significant activity enhancement | Homo sapiens | |
additional information | no singnificant activity with Li+, Cs+, Rb+ | Homo sapiens | |
Na+ | 6fold increase of activity | Homo sapiens | |
Na+ | activation. Affinity for ATP and pyridoxal is increased severalfold in presence of K+ compared with Na+, but the maximal activity of the Na+ form is more than double the activity of the K+ form | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O00764 | - |
- |
Purification (Comment) | Organism |
---|---|
TMAE column chromatography, Phenyl Sepharose column chromatography, and hydroxyapatite column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyridoxal | - |
Homo sapiens | ADP + pyridoxal 5'-phosphate | - |
? | |
ATP + pyridoxamine | - |
Homo sapiens | ADP + pyridoxamine 5'-phosphate | - |
? | |
ATP + pyridoxine | - |
Homo sapiens | ADP + pyridoxine 5'-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | x-ray crystallography | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
PLK | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.42 | - |
pyridoxal | presence of 40 mM K+, pH 7.3, 37°C | Homo sapiens | |
3.3 | - |
pyridoxal | presence of 40 mM Na+, pH 7.3, 37°C | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |