2.7.1.136: macrolide 2'-kinase
This is an abbreviated version!
For detailed information about macrolide 2'-kinase, go to the full flat file.
Word Map on EC 2.7.1.136
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2.7.1.136
-
erythromycin
-
oleandomycin
-
erythromycin-resistant
-
phosphotransferases
-
aminoglycoside
-
self-transferable
-
purine
-
roxithromycin
-
triphosphate
-
itp
-
repressor
-
16-membered
-
agarose
-
2'-phosphate
-
iodine
-
edta
-
macrolide-resistance
-
aeruginosa
-
analysis
- 2.7.1.136
- erythromycin
- oleandomycin
-
erythromycin-resistant
-
phosphotransferases
- aminoglycoside
-
self-transferable
- purine
- roxithromycin
- triphosphate
- itp
- repressor
-
16-membered
- agarose
- 2'-phosphate
- iodine
- edta
-
macrolide-resistance
- aeruginosa
- analysis
Reaction
Synonyms
macrolide 2'-phosphotransferase, macrolide 2'-phosphotransferase II, macrolide 2'-phosphotransferase type I, macrolide 2'-phosphotransferase type II, Mph, MPH (2'), MPH(2'), MPH(2')-I, MPH(2')-II, mphA, mphB, phosphotransferase, macrolide 2'-
ECTree
2.7.1.136 macrolide 2'-kinaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 2.7.1.136 - macrolide 2'-kinase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + josamycin
ADP + josamycin 2'-O-phosphate
-
15-membered ring macrolide
-
-
?
ATP + kitasamycin
?
-
52% activity with respect to oleandomycin for strain CU1, 61% activity with respect to oleandomycin for strain BM 2506
-
-
?
ATP + kitasamycin
ADP + kitasamycin 2'-O-phosphate
-
15-membered ring macrolide
-
-
?
ATP + rokitamycin
ADP + rokitamycin 2'-O-phosphate
-
15-membered ring macrolide
-
-
?
ATP + triacetyloleandomycin
?
-
100% activity with respect to oleandomycin
-
-
?
ATP + triacetyloleandomycin + H2O
ADP + triacetyloleandomycin 2'-O-phosphate + acetate
-
as active as oleandomycin
-
-
?
ATP + troleandomycin
?
-
82% activity with respect to oleandomycin for strain CU1, 105% activity with respect to oleandomycin for strain BM 2506
-
-
?
ATP + troleandomycin + H2O
ADP + troleandomycin 2'-O-phosphate + acetate
-
14-membered ring macrolide
-
-
?
ATP + azithromycin
ADP + azithromycin 2'-O-phosphate
-
15-membered ring macrolide
-
-
?
ATP + azithromycin
ADP + azithromycin 2'-O-phosphate
the mph(A) gene conferrs resistance to azithromycin
-
-
?
ATP + azithromycin
ADP + azithromycin 2'-O-phosphate
-
15-membered ring macrolide
-
-
?
ATP + azithromycin
ADP + azithromycin 2'-O-phosphate
-
15% of the activity with oleandomycin
-
-
?
ATP + azithromycin
ADP + azithromycin 2'-O-phosphate
the mph(A) gene conferrs resistance to azithromycin
-
-
?
ATP + azithromycin
ADP + azithromycin 2'-O-phosphate
the mph(A) gene conferrs resistance to azithromycin
-
-
?
ATP + azithromycin
ADP + azithromycin 2'-O-phosphate
recombinant expression of the pRSB111 plasmid in Pseudomonas sp. strain B13
-
-
?
ATP + clarithromycin
?
-
46-55% activity with respect to oleandomycin for strain CU1, 17% activity with respect to oleandomycin for strain BM 2506
-
-
?
ATP + clarithromycin
?
-
77% activity with respect to oleandomycin
-
-
?
ATP + clarithromycin
ADP + clarithromycin 2'-O-phosphate
-
14-membered ring macrolide
-
-
?
ATP + clarithromycin
ADP + clarithromycin 2'-O-phosphate
-
14-membered ring macrolide
-
-
?
ATP + clarithromycin
ADP + clarithromycin 2'-O-phosphate
-
73% of the activity with oleandomycin
-
-
?
ATP + clarithromycin
ADP + clarithromycin 2'-O-phosphate
recombinant expression of the pRSB111 plasmid in Pseudomonas sp. strain B13
-
-
?
ATP + erythromycin
ADP + erythromycin 2'-O-phosphate
-
14-membered ring macrolide
-
-
?
ATP + erythromycin
ADP + erythromycin 2'-O-phosphate
erythromycin resistance analyzed in recombinant Escherichia coli harbouring plasmid pRSB105
-
-
?
ATP + erythromycin
ADP + erythromycin 2'-O-phosphate
-
88% of the activity with oleandomycin
-
-
?
ATP + erythromycin
ADP + erythromycin-2'-O-phosphate
87% activity with respect to oleandomycin
-
-
?
ATP + erythromycin
ADP + erythromycin-2'-O-phosphate
-
81% activity with respect to oleandomycin for strain CU1, 24% activity with respect to oleandomycin for strain BM 2506
-
-
?
ATP + erythromycin
ADP + erythromycin-2'-O-phosphate
-
88% activity with respect to oleandomycin
-
-
?
ATP + erythromycin
ADP + erythromycin-2'-O-phosphate
-
-
-
-
?
ATP + erythromycin
ADP + erythromycin-2'-O-phosphate
recombinant expression of the pRSB111 plasmid in Pseudomonas sp. strain B13
-
-
?
ATP + josamycin
?
-
75-95% activity with respect to oleandomycin for strain CU1, 96% activity with respect to oleandomycin for strain BM 2506
-
-
?
ATP + oleandomycin
ADP + oleandomycin 2'-O-phosphate
-
14-membered ring macrolide
-
-
?
ATP + oleandomycin
ADP + oleandomycin 2'-O-phosphate
-
14-membered ring macrolide
-
-
?
ATP + oleandomycin
ADP + oleandomycin 2'-O-phosphate
-
-
-
?
ATP + oleandomycin
ADP + oleandomycin 2'-O-phosphate
-
-
-
?
ATP + oleandomycin
ADP + oleandomycin 2'-O-phosphate
-
-
-
?
ATP + roxithromycin
?
-
42% activity with respect to oleandomycin for strain CU1, about 2% activity with respect to oleandomycin for strain BM 2506
-
-
?
ATP + roxithromycin
?
-
44% activity with respect to oleandomycin
-
-
?
ATP + roxithromycin
ADP + roxithromycin 2'-O-phosphate
-
14-membered ring macrolide
-
-
?
ATP + roxithromycin
ADP + roxithromycin 2'-O-phosphate
-
44% of the activity with oleandomycin
-
-
?
ATP + roxithromycin
ADP + roxithromycin 2'-O-phosphate
recombinant expression of the pRSB111 plasmid in Pseudomonas sp. strain B13
-
-
?
ATP + spiramycin
ADP + spiramycin 2'-O-phosphate
-
57-75% activity with respect to oleandomycin for strain CU1, 26% activity with respect to oleandomycin for strain BM 2506
-
-
?
ATP + spiramycin
ADP + spiramycin 2'-O-phosphate
-
15-membered ring macrolide
-
-
?
ATP + spiramycin
ADP + spiramycin 2'-O-phosphate
-
15-membered ring macrolide
-
-
?
ATP + spiramycin
ADP + spiramycin 2'-O-phosphate
-
2% activity with respect to oleandomycin
-
-
?
ATP + telithromycin
ADP + telithromycin 2'-O-phosphate
the mph(A) gene conferrs resistance to telithromycin
-
-
?
ATP + telithromycin
ADP + telithromycin 2'-O-phosphate
the mph(A) gene conferrs resistance to telithromycin
-
-
?
ATP + telithromycin
ADP + telithromycin 2'-O-phosphate
the mph(A) gene conferrs resistance to telithromycin
-
-
?
ATP + tylosin
ADP + tylosin 2'-O-phosphate
-
15-membered ring macrolide
-
-
?
ATP + tylosin
ADP + tylosin 2'-O-phosphate
recombinant expression of the pRSB111 plasmid in Pseudomonas sp. strain B13
-
-
?
ATP + tylosin
ADP + tylosin 2'-phosphate
-
60% activity with respect to oleandomycin for strain CU1, 4% activity with respect to oleandomycin for strain BM 2506
-
-
?
ATP + tylosin
ADP + tylosin 2'-phosphate
-
2% activity with respect to oleandomycin
-
-
?
additional information
?
-
-
substrate specificities of wild-type and mutant enzymes, structure-function considerations
-
-
?
additional information
?
-
under physiological conditions, enzyme exclusively uses ATP, no substrate: GTP
-
-
?
additional information
?
-
structure-based substrate specificity, macrolide binding analysis, overview. Although erythromycin, clarithromycin, oleandomycin, and azithromycin differ in the size of the lactone ring and varying substitutions, the 14- and 15-membered ring substrates bind to the two MPH enzymes in a similar location, adopt similar conformations, and interact with the enzymes in a similar manner. MPH(2')-I cannot modify 16-membered macrolides, in contrast to MPH(2')-II
-
-
-
additional information
?
-
structure-based substrate specificity, macrolide binding analysis, overview. Although erythromycin, clarithromycin, oleandomycin, and azithromycin differ in the size of the lactone ring and varying substitutions, the 14- and 15-membered ring substrates bind to the two MPH enzymes in a similar location, adopt similar conformations, and interact with the enzymes in a similar manner. MPH(2')-I cannot modify 16-membered macrolides, in contrast to MPH(2')-II
-
-
-
additional information
?
-
structure-based substrate specificity, macrolide binding analysis, overview. Although erythromycin, clarithromycin, oleandomycin, and azithromycin differ in the size of the lactone ring and varying substitutions, the 14- and 15-membered ring substrates bind to the two MPH enzymes in a similar location, adopt similar conformations, and interact with the enzymes in a similar manner. Unlike MPH(2')-I, MPH(2')-II can efficiently modify 16-membered macrolides. The 16-membered rings are more ovoid in shape compared with the rounded shape of the 14- and 15-membered macrolides. The interactions between MPH(2')-II and the 16-membered macrolides, spiramycin and josamycin, are similar to those of the 14- and 15-membered macrolides, involving many of the same amino acid side chains
-
-
-
additional information
?
-
structure-based substrate specificity, macrolide binding analysis, overview. Although erythromycin, clarithromycin, oleandomycin, and azithromycin differ in the size of the lactone ring and varying substitutions, the 14- and 15-membered ring substrates bind to the two MPH enzymes in a similar location, adopt similar conformations, and interact with the enzymes in a similar manner. Unlike MPH(2')-I, MPH(2')-II can efficiently modify 16-membered macrolides. The 16-membered rings are more ovoid in shape compared with the rounded shape of the 14- and 15-membered macrolides. The interactions between MPH(2')-II and the 16-membered macrolides, spiramycin and josamycin, are similar to those of the 14- and 15-membered macrolides, involving many of the same amino acid side chains
-
-
-
additional information
?
-
-
substrate specificities of wild-type and mutant enzymes, structure-function considerations
-
-
?
