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Literature summary for 2.7.1.136 extracted from

  • Szczepanowski, R.; Krahn, I.; Bohn, N.; Puehler, A.; Schlueter, A.
    Novel macrolide resistance module carried by the IncP-1beta resistance plasmid pRSB111, isolated from a wastewater treatment plant (2007), Antimicrob. Agents Chemother., 51, 673-678.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.136

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli, strain DH5alpha mcr containing the pBCSK vector carrying a chloramphenicol resistance gene and the pRSB111 erythromycin resistance-conferring region, recombinant expression in Pseudomonas sp. strain B13 GFP1 uncultured bacterium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + azithromycin uncultured bacterium
-
 ADP + azithromycin 2'-O-phosphate
-
 ?
ATP + clarithromycin uncultured bacterium
-
 ADP + clarithromycin 2'-O-phosphate
-
 ?
ATP + erythromycin uncultured bacterium
-
 ADP + erythromycin-2'-O-phosphate
-
 ?
ATP + roxithromycin uncultured bacterium
-
 ADP + roxithromycin 2'-O-phosphate
-
 ?
ATP + tylosin uncultured bacterium
-
 ADP + tylosin 2'-O-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
uncultured bacterium Q5W3B4 macrolide resistance plasmid pRSB111, isolated from bacteria residing in the final effluents of a wastewater treatment plant
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 accessory region on plasmid pRSB111 contains a new macrolide resistance operon, mph(E) gene encodes a macrolide phosphotransferase required for high-level macrolide resistance, resistance analyzed by determining values for minimum inhibitory concentration uncultured bacterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + azithromycin
-
 uncultured bacterium ADP + azithromycin 2'-O-phosphate
-
 ?
ATP + azithromycin recombinant expression of the pRSB111 plasmid in Pseudomonas sp. strain B13 uncultured bacterium ADP + azithromycin 2'-O-phosphate
-
 ?
ATP + clarithromycin
-
 uncultured bacterium ADP + clarithromycin 2'-O-phosphate
-
 ?
ATP + clarithromycin recombinant expression of the pRSB111 plasmid in Pseudomonas sp. strain B13 uncultured bacterium ADP + clarithromycin 2'-O-phosphate
-
 ?
ATP + erythromycin
-
 uncultured bacterium ADP + erythromycin-2'-O-phosphate
-
 ?
ATP + erythromycin recombinant expression of the pRSB111 plasmid in Pseudomonas sp. strain B13 uncultured bacterium ADP + erythromycin-2'-O-phosphate
-
 ?
ATP + roxithromycin
-
 uncultured bacterium ADP + roxithromycin 2'-O-phosphate
-
 ?
ATP + roxithromycin recombinant expression of the pRSB111 plasmid in Pseudomonas sp. strain B13 uncultured bacterium ADP + roxithromycin 2'-O-phosphate
-
 ?
ATP + tylosin
-
 uncultured bacterium ADP + tylosin 2'-O-phosphate
-
 ?
ATP + tylosin recombinant expression of the pRSB111 plasmid in Pseudomonas sp. strain B13 uncultured bacterium ADP + tylosin 2'-O-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
macrolide 2'-phosphotransferase
-
 uncultured bacterium