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2.7.1.136: macrolide 2'-kinase

This is an abbreviated version!
For detailed information about macrolide 2'-kinase, go to the full flat file.

Word Map on EC 2.7.1.136

Reaction

ATP
+
oleandomycin
=
ADP
 +
oleandomycin 2'-O-phosphate

Synonyms

macrolide 2'-phosphotransferase, macrolide 2'-phosphotransferase II, macrolide 2'-phosphotransferase type I, macrolide 2'-phosphotransferase type II, Mph, MPH (2'), MPH(2'), MPH(2')-I, MPH(2')-II, mphA, mphB, phosphotransferase, macrolide 2'-

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.136 macrolide 2'-kinase

Cloned

Cloned on EC 2.7.1.136 - macrolide 2'-kinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli DH5alpha, complete pRSB105 macrolide resistance gene region cloned into the vector pBCSK, recombinant plasmid pNK-mph carries the complete mph gene in pBluescript II KS, transcription from the constructed plasmids analyzed by RT-PCR, constructs tested for conferring erythromycin and azithromycin resistance
expressed in Escherichia coli, strain DH5alpha mcr containing the pBCSK vector carrying a chloramphenicol resistance gene and the pRSB111 erythromycin resistance-conferring region, recombinant expression in Pseudomonas sp. strain B13 GFP1
expression of wild-type and mutant enzymes in strains TG1 and N43
-
mph gene for Escherichia coli strain CU1 is identical to that previously reported for mphB from Escherichia coli strain BM 2506
-
mph genes cloned into Escherichia coli AG100A
mphA gene is arranged in close proximity to mrx and MphR(A) which are proteins probably involved in mph regulation, they all form a gene cluster