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2.7.1.11: 6-phosphofructokinase

This is an abbreviated version!
For detailed information about 6-phosphofructokinase, go to the full flat file.

Word Map on EC 2.7.1.11

Reaction

ATP
+
beta-D-fructofuranose 6-phosphate
=
ADP
 +
beta-D-fructofuranose 1,6-bisphosphate

Synonyms

1-phosphofructokinase, 2-keto-3-deoxygluconate kinase, 6-phosphofructo 1-kinase, 6-phosphofructo-1-kinase, 6-phosphofructokinase I, 6-phosphofructokinase, platelet type, 6-phosphofructose 1-kinase, 6-phosphofructose-1-kinase, A/GTP-PFK, ADP-dependent phosphofructokinase, APE0012, At2g22480, At4g26270, At4g29220, At4g32840, At5g56630, At5g61580, ATP-dependent 6-phosphofructokinase, ATP-dependent 6-phosphofructokinase isozyme 2, ATP-dependent PFK, ATP-dependent phosphofructokinase, ATP-PFK, ATP: D-fructose-6-phosphate-1-phosphotransferase, ATP:D-fructose-6-phosphate-1-phosphotransferase, D-fructose-6-phosphate 1-phosphotransferase, fructose 6-phosphate kinase, fructose 6-phosphokinase, hosphofructokinase-1, HVO_A0328, KDG kinase, KDGal kinase, KDGK-1, KDGK-2, kinase, phosphofructo- (phosphorylating), LmPFK, muscle-type phosphofructokinase, nucleotide triphosphate-dependent phosphofructokinase, OC_Os04g39420, PFK, PFK-1, Pfk-2, PFK-L, PFK-M, PFK-P, PFK1, PFK2, PFK3, PFK4, PFK5, PFK6, PFK7, PFKA, PFKA1, PFKA2, PFKA3, pfkB, PFKL, PFKM, PFKMS, PFKP, PfpA4, phospho-1,6-fructokinase, phosphofructo-1-kinase, phosphofructokinase, phosphofructokinase 1, phosphofructokinase M, phosphofructokinase-1, phosphofructokinase-2, phosphofructokinase-M, phosphofructokinase-P, phosphohexokinase, TbPFK, TcPFK

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.11 6-phosphofructokinase

General Stability

General Stability on EC 2.7.1.11 - 6-phosphofructokinase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
(NH4)2SO4 prevents enzyme from dissociation at pH 6.4
-
10% glycerol, dithiothreitol and PMSF stabilize during purification
-
50 mM phosphate stabilizes at pH 6.6
-
AMP, ADP, fructose 6-phosphate or antipain stabilizes during purification
-
at least 15 mM phosphate required to stabilize PFK during purification, presence of 10% glycerol helps to stabilize
-
ATP stabilizes
-
cold labile, fructose 1,6-bisphosphate stabilizes
-
concentration by ultrafiltration on Amicon PM-10 membrane results in appreciable loss of activity, concentration in collodium bags allows higher protein concentration to be obtained without loss of activity
-
cytosolic enzyme extremely unstable upon purification, substrates, metabolites, ethylene glycol or SH-compounds do not stabilize
-
cytosolic enzymes are less stable than plastidic
-
D-fructose 6-phosphate stabilizes, less effective is KCl or phosphoenolpyruvate, only slightly stabilized by ATP or ATP/Mg2+
dilution inactivates
dilution leads to enzyme dissociation
dilution reversibly decreases activity
dithioerythritol stabilizes
-
freeze-drying inactivates, addition of Zn2+ plus trehalose, maltose, sucrose, galactose or glucose stabilizes, more than 80% of the initial activity is retained in the presence of 0.4 mM Zn2+ and 100 mM trehalose, no stabilization in the presence of glucose or galactose or any other divalent cation alone
-
fructose 6-phosphate stabilizes, not 1 mM ATP or 50% v/v glycerol
-
fructose 6-phosphate, ATP or ammonium sulfate stabilizes during dialysis and storage at pH 6-9
-
fructose 6-phosphate, ATP, ADP, citrate, KCl or ammonium sulfate does not stabilize
-
fructose 6-phosphate, fructose 1,6-bisphosphate and ammonium sulfate stabilize dilute enzyme solutions
-
fructose 6-phosphate, MgCl2 and ethylene glycol stabilize
-
glycerol and KCl or ATP stabilize
glycerol prevents inactivation of dilute cytosolic enzyme solution
glycerol stabilizes
-
glycine, proline, hydroxyproline, trimethylamine N-oxide, glycerol or myo-inositol affords a high degree of cryoprotection
-
labile at all stages of purification, particularly at high dilutions or high salt concentrations, glycerol, fructose 6-phosphate and high enzyme concentrations stabilize
-
largest molecular weight-form of purified enzyme is the most stable form
-
leaf cytosolic isozyme rather unstable upon purification
-
MgATP2- or ATP and Mg2+ stabilize during purification
-
NaF, EDTA and PMSF stabilize during purification
-
PMSF stabilizes during purification
-
polyethylene glycol stabilizes
-
sensitive to freezing, complete inactivation after 24 h at -20°C at pH 7.5
-
unstable in buffers like imidazole-HCl, pH 6.6 or 6.8, or Tris-HCl, pH 8, 50 mM phosphate and 0.3 M KCl stabilize
-
unstable to vigorous sonication or lyophilization
-
very stable in Tris-phosphate or glycylglycine-glycerol buffer, pH 8, at 10-20 mg protein per ml
-
very unstable upon purification, dithioerythritol, K+ or Mg2+ and phosphate partially stabilize
Dunaliella marina
-