Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Rattus norvegicus | |
ADP | - |
Saccharomyces cerevisiae | |
ADP | - |
Clostridium pasteurianum | |
ADP | - |
Fasciola hepatica | |
AMP | - |
Rattus norvegicus | |
AMP | - |
Saccharomyces cerevisiae | |
AMP | - |
Bos taurus | |
AMP | - |
Fasciola hepatica | |
cAMP | - |
Rattus norvegicus | |
cAMP | - |
Oryctolagus cuniculus | |
cAMP | - |
Fasciola hepatica | |
glutathione | activation | Oryctolagus cuniculus | |
NH4+ | - |
Escherichia coli | |
NH4+ | - |
Saccharomyces cerevisiae | |
NH4+ | - |
Oryctolagus cuniculus | |
NH4+ | - |
Clostridium pasteurianum | |
NH4+ | - |
Fasciola hepatica | |
NH4+ | activation of muscle enzyme, inhibitory at high concentrations | Rattus norvegicus |
Crystallization (Comment) | Organism |
---|---|
- |
Gallus gallus |
- |
Saccharomyces cerevisiae |
- |
Oryctolagus cuniculus |
- |
Ovis aries |
- |
Clostridium pasteurianum |
General Stability | Organism |
---|---|
dilution reversibly decreases activity | Oryctolagus cuniculus |
dilution reversibly decreases activity | Glutamicibacter nicotianae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | brain PFK, not heart PFK | Rattus norvegicus | |
ADP | - |
Clostridium pasteurianum | |
ADP | plant enzymes | Pisum sativum | |
ADP | plant enzymes | Zea mays | |
AMP | - |
Brassica oleracea var. gemmifera | |
AMP | weak inhibition | Daucus carota | |
AMP | weak inhibition | Lactiplantibacillus plantarum | |
AMP | weak inhibition | Neurospora crassa | |
AMP | weak inhibition | Pisum sativum | |
AMP | - |
Zea mays | |
Antibodies against rabbit muscle enzyme | not rabbit erythrocyte, leukocyte or platelet enzyme | Oryctolagus cuniculus | |
ATP | - |
Bos taurus | |
ATP | - |
Escherichia coli | |
ATP | - |
Fasciola hepatica | |
ATP | - |
Homo sapiens | |
ATP | ADP partially reverses inhibition | Klebsiella aerogenes | |
ATP | ADP partially reverses inhibition; cAMP partially reverses inhibition; fructose 1,6-bisphosphate partially reverses inhibition; phosphate and AMP partially reverse inhibition | Mus musculus | |
ATP | muscle PFK, strong inhibition at pH 7.1, weak inhibition at pH 7.6-8.5, not inhibited at pH 9.0 | Oryctolagus cuniculus | |
ATP | - |
Ovis aries | |
ATP | fructose 1,6-bisphosphate partially reverses inhibition; phosphate and AMP partially reverse inhibition | Rattus norvegicus | |
ATP | - |
Saccharomyces cerevisiae | |
cAMP | - |
Lactiplantibacillus plantarum | |
cis-aconitate | brain, not heart | Rattus norvegicus | |
citrate | strong inhibition | Arthrobacter crystallopoietes | |
citrate | strong inhibition | Bos taurus | |
citrate | strong inhibition | Brassica oleracea var. gemmifera | |
citrate | strong inhibition | Clostridium pasteurianum | |
citrate | strong inhibition | Daucus carota | |
citrate | strong inhibition | Dictyostelium discoideum | |
citrate | strong inhibition | Escherichia coli | |
citrate | strong inhibition | Fasciola hepatica | |
citrate | strong inhibition | Gallus gallus | |
citrate | strong inhibition | Glutamicibacter nicotianae | |
citrate | strong inhibition | Homo sapiens | |
citrate | strong inhibition | Klebsiella aerogenes | |
citrate | strong inhibition | Lacticaseibacillus casei | |
citrate | strong inhibition | Lactiplantibacillus plantarum | |
citrate | strong inhibition | Mus musculus | |
citrate | strong inhibition | Neurospora crassa | |
citrate | strong inhibition | Oryctolagus cuniculus | |
citrate | strong inhibition | Ovis aries | |
citrate | strong inhibition | Pisum sativum | |
citrate | strong inhibition; synergistic with phosphate and AMP | Rattus norvegicus | |
citrate | strong inhibition | Saccharomyces cerevisiae | |
citrate | strong inhibition | Thermus thermophilus | |
citrate | strong inhibition | Zea mays | |
D-fructose 1,6-bisphosphate | - |
Clostridium pasteurianum | |
D-fructose 1,6-bisphosphate | - |
Dictyostelium discoideum | |
D-Fructose 1-phosphate | - |
Arthrobacter crystallopoietes | |
D-Fructose 1-phosphate | - |
Bos taurus | |
D-Fructose 1-phosphate | - |
Brassica oleracea var. gemmifera | |
D-Fructose 1-phosphate | - |
Clostridium pasteurianum | |
D-Fructose 1-phosphate | - |
Daucus carota | |
D-Fructose 1-phosphate | - |
Dictyostelium discoideum | |
D-Fructose 1-phosphate | - |
Escherichia coli | |
D-Fructose 1-phosphate | - |
Fasciola hepatica | |
D-Fructose 1-phosphate | - |
Gallus gallus | |
D-Fructose 1-phosphate | - |
Glutamicibacter nicotianae | |
D-Fructose 1-phosphate | - |
Homo sapiens | |
D-Fructose 1-phosphate | - |
Klebsiella aerogenes | |
D-Fructose 1-phosphate | - |
Lacticaseibacillus casei | |
D-Fructose 1-phosphate | - |
Lactiplantibacillus plantarum | |
D-Fructose 1-phosphate | - |
Mus musculus | |
D-Fructose 1-phosphate | - |
Neurospora crassa | |
D-Fructose 1-phosphate | - |
Oryctolagus cuniculus | |
D-Fructose 1-phosphate | - |
Ovis aries | |
D-Fructose 1-phosphate | - |
Pisum sativum | |
D-Fructose 1-phosphate | - |
Rattus norvegicus | |
D-Fructose 1-phosphate | - |
Saccharomyces cerevisiae | |
D-Fructose 1-phosphate | - |
Thermus thermophilus | |
D-Fructose 1-phosphate | - |
Zea mays | |
isocitrate | brain, not heart | Rattus norvegicus | |
malate | kidney cortex, brain, not heart | Rattus norvegicus | |
Maleic anhydride | muscle and heart enzymes, mechanism | Mus musculus | |
Maleic anhydride | muscle and heart enzymes, mechanism | Oryctolagus cuniculus | |
Maleic anhydride | muscle and heart enzymes, mechanism | Rattus norvegicus | |
Mg2+ | brain enzyme, at high concentrations | Mus musculus | |
Mg2+ | brain enzyme, at high concentrations | Oryctolagus cuniculus | |
Mg2+ | brain enzyme, at high concentrations | Rattus norvegicus | |
additional information | not inhibited by ATP; not inhibited by fructose 1,6-bisphosphate | Arthrobacter crystallopoietes | |
additional information | not inhibited by fructose 1,6-bisphosphate | Bos taurus | |
additional information | not inhibited by fructose 1,6-bisphosphate | Brassica oleracea var. gemmifera | |
additional information | not inhibited by fructose 1,6-bisphosphate | Clostridium pasteurianum | |
additional information | not inhibited by fructose 1,6-bisphosphate | Daucus carota | |
additional information | not inhibited by ATP; not inhibited by fructose 1,6-bisphosphate | Dictyostelium discoideum | |
additional information | not inhibited by fructose 1,6-bisphosphate; not inhibited by ITP, fumarate, tricarballylic acid, CoA, acetyl-CoA | Escherichia coli | |
additional information | not inhibited by fructose 1,6-bisphosphate | Fasciola hepatica | |
additional information | not inhibited by fructose 1,6-bisphosphate; not inhibited by ITP, fumarate, tricarballylic acid, CoA, acetyl-CoA | Gallus gallus | |
additional information | not inhibited by fructose 1,6-bisphosphate | Glutamicibacter nicotianae | |
additional information | not inhibited by fructose 1,6-bisphosphate | Homo sapiens | |
additional information | not inhibited by fructose 1,6-bisphosphate | Klebsiella aerogenes | |
additional information | not inhibited by ATP; not inhibited by fructose 1,6-bisphosphate | Lacticaseibacillus casei | |
additional information | not inhibited by ATP; not inhibited by fructose 1,6-bisphosphate | Lactiplantibacillus plantarum | |
additional information | not inhibited by fructose 1,6-bisphosphate; not inhibited by ITP, fumarate, tricarballylic acid, CoA, acetyl-CoA; photooxidation yields a new heart enzyme species that is no longer sensitive to ATP | Mus musculus | |
additional information | not inhibited by fructose 1,6-bisphosphate | Neurospora crassa | |
additional information | not inhibited by fructose 1,6-bisphosphate; not inhibited by ITP, fumarate, tricarballylic acid, CoA, acetyl-CoA; photooxidation yields a new heart enzyme species that is no longer sensitive to ATP | Oryctolagus cuniculus | |
additional information | not inhibited by fructose 1,6-bisphosphate | Ovis aries | |
additional information | not inhibited by fructose 1,6-bisphosphate | Pisum sativum | |
additional information | heart enzyme, not inhibited by cis-aconitate, L-isocitrate, alpha-ketoglutarate, succinate, fumarate, malate, tricarnallylic acid, CoASH, or acetyl-CoASH; not inhibited by fructose 1,6-bisphosphate; not inhibited by ITP, fumarate, tricarballylic acid, CoA, acetyl-CoA; photooxidation yields a new heart enzyme species that is no longer sensitive to ATP | Rattus norvegicus | |
additional information | not inhibited by fructose 1,6-bisphosphate; not inhibited by GTP; not inhibited by ITP, fumarate, tricarballylic acid, CoA, acetyl-CoA | Saccharomyces cerevisiae | |
additional information | not inhibited by fructose 1,6-bisphosphate | Thermus thermophilus | |
additional information | not inhibited by fructose 1,6-bisphosphate | Zea mays | |
NH4+ | muscle PFK, weak, at high concentrations, activation at very low concentrations | Oryctolagus cuniculus | |
phosphoenolpyruvate | - |
Escherichia coli | |
phosphoenolpyruvate | - |
Mus musculus | |
phosphoenolpyruvate | - |
Rattus norvegicus | |
pyridoxal 5'-phosphate | muscle and heart enzymes, mechanism | Mus musculus | |
pyridoxal 5'-phosphate | muscle and heart enzymes, mechanism | Oryctolagus cuniculus | |
pyridoxal 5'-phosphate | muscle and heart enzymes, mechanism | Rattus norvegicus | |
succinate | kidney cortex and brain PFK, heart PFk is not inhibited | Rattus norvegicus | |
Succinic anhydride | muscle and heart enzymes, mechanism | Mus musculus | |
Succinic anhydride | muscle and heart enzymes, mechanism | Oryctolagus cuniculus | |
Succinic anhydride | muscle and heart enzymes, mechanism | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic data of various organism | Gallus gallus | |
additional information | - |
additional information | kinetic data of various organism | Lactiplantibacillus plantarum | |
additional information | - |
additional information | kinetic data of various organism | Mus musculus | |
additional information | - |
additional information | kinetic data of various organism | Thermus thermophilus | |
additional information | - |
additional information | kinetic data of various organism | Escherichia coli | |
additional information | - |
additional information | kinetic data of various organism | Homo sapiens | |
additional information | - |
additional information | kinetic data of various organism | Rattus norvegicus | |
additional information | - |
additional information | kinetic data of various organism | Saccharomyces cerevisiae | |
additional information | - |
additional information | kinetic data of various organism | Bos taurus | |
additional information | - |
additional information | kinetic data of various organism | Oryctolagus cuniculus | |
additional information | - |
additional information | kinetic data of various organism | Neurospora crassa | |
additional information | - |
additional information | kinetic data of various organism | Ovis aries | |
additional information | - |
additional information | kinetic data of various organism | Pisum sativum | |
additional information | - |
additional information | kinetic data of various organism | Zea mays | |
additional information | - |
additional information | kinetic data of various organism | Klebsiella aerogenes | |
additional information | - |
additional information | kinetic data of various organism | Dictyostelium discoideum | |
additional information | - |
additional information | kinetic data of various organism | Lacticaseibacillus casei | |
additional information | - |
additional information | kinetic data of various organism | Clostridium pasteurianum | |
additional information | - |
additional information | kinetic data of various organism | Daucus carota | |
additional information | - |
additional information | kinetic data of various organism | Glutamicibacter nicotianae | |
additional information | - |
additional information | kinetic data of various organism | Fasciola hepatica | |
additional information | - |
additional information | kinetic data of various organism | Arthrobacter crystallopoietes | |
additional information | - |
additional information | kinetic data of various organism | Brassica oleracea var. gemmifera |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Gallus gallus | 5829 | - |
cytosol | - |
Lactiplantibacillus plantarum | 5829 | - |
cytosol | - |
Mus musculus | 5829 | - |
cytosol | - |
Thermus thermophilus | 5829 | - |
cytosol | - |
Escherichia coli | 5829 | - |
cytosol | - |
Homo sapiens | 5829 | - |
cytosol | - |
Rattus norvegicus | 5829 | - |
cytosol | - |
Saccharomyces cerevisiae | 5829 | - |
cytosol | - |
Bos taurus | 5829 | - |
cytosol | - |
Oryctolagus cuniculus | 5829 | - |
cytosol | - |
Neurospora crassa | 5829 | - |
cytosol | - |
Ovis aries | 5829 | - |
cytosol | - |
Pisum sativum | 5829 | - |
cytosol | - |
Zea mays | 5829 | - |
cytosol | - |
Klebsiella aerogenes | 5829 | - |
cytosol | - |
Dictyostelium discoideum | 5829 | - |
cytosol | - |
Lacticaseibacillus casei | 5829 | - |
cytosol | - |
Clostridium pasteurianum | 5829 | - |
cytosol | - |
Daucus carota | 5829 | - |
cytosol | - |
Glutamicibacter nicotianae | 5829 | - |
cytosol | - |
Fasciola hepatica | 5829 | - |
cytosol | - |
Arthrobacter crystallopoietes | 5829 | - |
cytosol | - |
Brassica oleracea var. gemmifera | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Gallus gallus | |
Co2+ | - |
Lactiplantibacillus plantarum | |
Co2+ | - |
Mus musculus | |
Co2+ | - |
Thermus thermophilus | |
Co2+ | - |
Escherichia coli | |
Co2+ | - |
Homo sapiens | |
Co2+ | - |
Rattus norvegicus | |
Co2+ | - |
Saccharomyces cerevisiae | |
Co2+ | - |
Bos taurus | |
Co2+ | - |
Oryctolagus cuniculus | |
Co2+ | - |
Neurospora crassa | |
Co2+ | - |
Ovis aries | |
Co2+ | - |
Pisum sativum | |
Co2+ | - |
Zea mays | |
Co2+ | - |
Klebsiella aerogenes | |
Co2+ | - |
Dictyostelium discoideum | |
Co2+ | - |
Lacticaseibacillus casei | |
Co2+ | - |
Clostridium pasteurianum | |
Co2+ | - |
Daucus carota | |
Co2+ | - |
Glutamicibacter nicotianae | |
Co2+ | - |
Fasciola hepatica | |
Co2+ | - |
Arthrobacter crystallopoietes | |
Co2+ | - |
Brassica oleracea var. gemmifera | |
K+ | - |
Lactiplantibacillus plantarum | |
K+ | - |
Mus musculus | |
K+ | - |
Thermus thermophilus | |
K+ | - |
Escherichia coli | |
K+ | - |
Rattus norvegicus | |
K+ | - |
Saccharomyces cerevisiae | |
K+ | - |
Neurospora crassa | |
K+ | - |
Pisum sativum | |
K+ | - |
Zea mays | |
K+ | - |
Klebsiella aerogenes | |
K+ | - |
Dictyostelium discoideum | |
K+ | - |
Lacticaseibacillus casei | |
K+ | - |
Clostridium pasteurianum | |
K+ | - |
Daucus carota | |
K+ | - |
Glutamicibacter nicotianae | |
K+ | - |
Fasciola hepatica | |
K+ | - |
Arthrobacter crystallopoietes | |
K+ | - |
Brassica oleracea var. gemmifera | |
Mg2+ | required for activity | Gallus gallus | |
Mg2+ | required for activity | Lactiplantibacillus plantarum | |
Mg2+ | required for activity | Mus musculus | |
Mg2+ | required for activity | Thermus thermophilus | |
Mg2+ | required for activity | Escherichia coli | |
Mg2+ | required for activity | Homo sapiens | |
Mg2+ | required for activity | Rattus norvegicus | |
Mg2+ | required for activity | Saccharomyces cerevisiae | |
Mg2+ | required for activity | Bos taurus | |
Mg2+ | required for activity | Oryctolagus cuniculus | |
Mg2+ | required for activity | Neurospora crassa | |
Mg2+ | required for activity | Ovis aries | |
Mg2+ | required for activity | Pisum sativum | |
Mg2+ | required for activity | Zea mays | |
Mg2+ | required for activity | Klebsiella aerogenes | |
Mg2+ | required for activity | Dictyostelium discoideum | |
Mg2+ | required for activity | Lacticaseibacillus casei | |
Mg2+ | required for activity | Clostridium pasteurianum | |
Mg2+ | required for activity | Daucus carota | |
Mg2+ | required for activity | Glutamicibacter nicotianae | |
Mg2+ | required for activity | Fasciola hepatica | |
Mg2+ | required for activity | Arthrobacter crystallopoietes | |
Mg2+ | required for activity | Brassica oleracea var. gemmifera | |
Mg2+ | MgATP is the active substrate | Gallus gallus | |
Mg2+ | MgATP is the active substrate | Lactiplantibacillus plantarum | |
Mg2+ | MgATP is the active substrate | Mus musculus | |
Mg2+ | MgATP is the active substrate | Thermus thermophilus | |
Mg2+ | MgATP is the active substrate | Escherichia coli | |
Mg2+ | MgATP is the active substrate | Homo sapiens | |
Mg2+ | MgATP is the active substrate | Rattus norvegicus | |
Mg2+ | MgATP is the active substrate | Saccharomyces cerevisiae | |
Mg2+ | MgATP is the active substrate | Bos taurus | |
Mg2+ | MgATP is the active substrate | Oryctolagus cuniculus | |
Mg2+ | MgATP is the active substrate | Neurospora crassa | |
Mg2+ | MgATP is the active substrate | Ovis aries | |
Mg2+ | MgATP is the active substrate | Pisum sativum | |
Mg2+ | MgATP is the active substrate | Zea mays | |
Mg2+ | MgATP is the active substrate | Klebsiella aerogenes | |
Mg2+ | MgATP is the active substrate | Dictyostelium discoideum | |
Mg2+ | MgATP is the active substrate | Lacticaseibacillus casei | |
Mg2+ | MgATP is the active substrate | Clostridium pasteurianum | |
Mg2+ | MgATP is the active substrate | Daucus carota | |
Mg2+ | MgATP is the active substrate | Glutamicibacter nicotianae | |
Mg2+ | MgATP is the active substrate | Fasciola hepatica | |
Mg2+ | MgATP is the active substrate | Arthrobacter crystallopoietes | |
Mg2+ | MgATP is the active substrate | Brassica oleracea var. gemmifera | |
Mg2+ | most effective ion | Gallus gallus | |
Mg2+ | most effective ion | Lactiplantibacillus plantarum | |
Mg2+ | most effective ion | Mus musculus | |
Mg2+ | most effective ion | Thermus thermophilus | |
Mg2+ | most effective ion | Escherichia coli | |
Mg2+ | most effective ion | Homo sapiens | |
Mg2+ | most effective ion | Rattus norvegicus | |
Mg2+ | most effective ion | Saccharomyces cerevisiae | |
Mg2+ | most effective ion | Bos taurus | |
Mg2+ | most effective ion | Oryctolagus cuniculus | |
Mg2+ | most effective ion | Neurospora crassa | |
Mg2+ | most effective ion | Ovis aries | |
Mg2+ | most effective ion | Pisum sativum | |
Mg2+ | most effective ion | Zea mays | |
Mg2+ | most effective ion | Klebsiella aerogenes | |
Mg2+ | most effective ion | Dictyostelium discoideum | |
Mg2+ | most effective ion | Lacticaseibacillus casei | |
Mg2+ | most effective ion | Clostridium pasteurianum | |
Mg2+ | most effective ion | Daucus carota | |
Mg2+ | most effective ion | Glutamicibacter nicotianae | |
Mg2+ | most effective ion | Fasciola hepatica | |
Mg2+ | most effective ion | Arthrobacter crystallopoietes | |
Mg2+ | most effective ion | Brassica oleracea var. gemmifera | |
Mn2+ | activation | Gallus gallus | |
Mn2+ | activation | Lactiplantibacillus plantarum | |
Mn2+ | activation | Mus musculus | |
Mn2+ | activation | Thermus thermophilus | |
Mn2+ | activation | Escherichia coli | |
Mn2+ | activation | Homo sapiens | |
Mn2+ | activation | Rattus norvegicus | |
Mn2+ | activation | Saccharomyces cerevisiae | |
Mn2+ | activation | Bos taurus | |
Mn2+ | activation | Oryctolagus cuniculus | |
Mn2+ | activation | Neurospora crassa | |
Mn2+ | activation | Ovis aries | |
Mn2+ | activation | Pisum sativum | |
Mn2+ | activation | Zea mays | |
Mn2+ | activation | Klebsiella aerogenes | |
Mn2+ | activation | Dictyostelium discoideum | |
Mn2+ | activation | Lacticaseibacillus casei | |
Mn2+ | activation | Clostridium pasteurianum | |
Mn2+ | activation | Daucus carota | |
Mn2+ | activation | Glutamicibacter nicotianae | |
Mn2+ | activation | Fasciola hepatica | |
Mn2+ | activation | Arthrobacter crystallopoietes | |
Mn2+ | activation | Brassica oleracea var. gemmifera |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
4 * 35000, isoenzyme PFK1, tetrahedral arranged subunits, SDS-PAGE | Escherichia coli |
36500 | - |
x * 36500, sedimentation equilibrium centrifugation in 6 M guanidine | Escherichia coli |
67000 | - |
6 * 67000, muscle enzyme, sedimentation equilibrium ultracentrifugation or gel filtration in 6.5 M guanidine | Oryctolagus cuniculus |
141000 | 142000 | - |
Escherichia coli |
144000 | - |
- |
Clostridium pasteurianum |
300000 | - |
gel filtration | Mus musculus |
380000 | - |
- |
Rattus norvegicus |
380000 | - |
skeletal muscle | Oryctolagus cuniculus |
500000 | - |
erythrocyte, sucrose density gradient centrifugation | Oryctolagus cuniculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | Gallus gallus | - |
ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | Lactiplantibacillus plantarum | - |
ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | Mus musculus | - |
ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | Thermus thermophilus | - |
ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | Escherichia coli | - |
ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | Homo sapiens | - |
ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | Rattus norvegicus | - |
ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | Saccharomyces cerevisiae | - |
ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | Bos taurus | - |
ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | Oryctolagus cuniculus | - |
ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | Neurospora crassa | - |
ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | Ovis aries | - |
ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | Pisum sativum | - |
ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | Zea mays | - |
ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | Klebsiella aerogenes | - |
ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | Dictyostelium discoideum | - |
ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | Lacticaseibacillus casei | - |
ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | Clostridium pasteurianum | - |
ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | Daucus carota | - |
ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | Glutamicibacter nicotianae | - |
ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | Fasciola hepatica | - |
ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | Arthrobacter crystallopoietes | - |
ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | Brassica oleracea var. gemmifera | - |
ADP + D-fructose 1,6-bisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter crystallopoietes | - |
- |
- |
Bos taurus | - |
ox | - |
Bos taurus | - |
calf | - |
Brassica oleracea var. gemmifera | - |
Brussel's sprout | - |
Clostridium pasteurianum | - |
- |
- |
Daucus carota | - |
carrot | - |
Dictyostelium discoideum | - |
- |
- |
Escherichia coli | - |
- |
- |
Fasciola hepatica | - |
liver fluke, from bile duct of infected cattle | - |
Gallus gallus | - |
- |
- |
Glutamicibacter nicotianae | - |
- |
- |
Homo sapiens | - |
- |
- |
Klebsiella aerogenes | - |
- |
- |
Lacticaseibacillus casei | - |
- |
- |
Lactiplantibacillus plantarum | - |
- |
- |
Mus musculus | - |
- |
- |
Neurospora crassa | - |
- |
- |
Oryctolagus cuniculus | - |
- |
- |
Ovis aries | - |
- |
- |
Pisum sativum | - |
pea | - |
Rattus norvegicus | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Thermus thermophilus | - |
- |
- |
Zea mays | - |
maize | - |
Purification (Comment) | Organism |
---|---|
- |
Mus musculus |
- |
Escherichia coli |
- |
Homo sapiens |
- |
Saccharomyces cerevisiae |
- |
Bos taurus |
- |
Oryctolagus cuniculus |
- |
Clostridium pasteurianum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adipose tissue | - |
Rattus norvegicus | - |
brain | - |
Homo sapiens | - |
brain | - |
Rattus norvegicus | - |
brain | - |
Ovis aries | - |
Ehrlich ascites carcinoma cell | - |
Mus musculus | - |
erythrocyte | - |
Homo sapiens | - |
erythrocyte | - |
Oryctolagus cuniculus | - |
heart | - |
Bos taurus | - |
heart | - |
Oryctolagus cuniculus | - |
heart | - |
Ovis aries | - |
kidney | cortex | Oryctolagus cuniculus | - |
leaf | - |
Brassica oleracea var. gemmifera | - |
lens | calf | Bos taurus | - |
leukocyte | - |
Homo sapiens | - |
liver | - |
Gallus gallus | - |
liver | - |
Homo sapiens | - |
liver | - |
Oryctolagus cuniculus | - |
muscle | - |
Homo sapiens | - |
muscle | - |
Rattus norvegicus | - |
muscle | - |
Bos taurus | - |
muscle | - |
Oryctolagus cuniculus | - |
muscle | - |
Ovis aries | - |
mycelium | - |
Neurospora crassa | - |
platelet | - |
Homo sapiens | - |
root | - |
Daucus carota | - |
seed | - |
Pisum sativum | - |
seed | - |
Daucus carota | - |
thyroid gland | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
93 | - |
heart muscle PFK | Bos taurus |
99.5 | - |
human skeletal muscle | Homo sapiens |
114 | 116 | - |
Gallus gallus |
114 | 116 | - |
Saccharomyces cerevisiae |
120 | 160 | skeletal muscle PFK | Oryctolagus cuniculus |
139 | - |
erythrocyte enzyme | Oryctolagus cuniculus |
150 | - |
- |
Mus musculus |
160 | - |
- |
Oryctolagus cuniculus |
160 | - |
- |
Clostridium pasteurianum |
160 | - |
heart PFK | Ovis aries |
189 | 215 | - |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,N6-etheno-ATP + D-fructose 6-phosphate | - |
Gallus gallus | 1,N6-etheno-ADP + D-fructose 1,6-bisphosphate | - |
? | |
1,N6-etheno-ATP + D-fructose 6-phosphate | - |
Mus musculus | 1,N6-etheno-ADP + D-fructose 1,6-bisphosphate | - |
? | |
1,N6-etheno-ATP + D-fructose 6-phosphate | - |
Rattus norvegicus | 1,N6-etheno-ADP + D-fructose 1,6-bisphosphate | - |
? | |
1,N6-etheno-ATP + D-fructose 6-phosphate | - |
Oryctolagus cuniculus | 1,N6-etheno-ADP + D-fructose 1,6-bisphosphate | - |
? | |
2-amino-9-beta-D-ribofuranosylpurine 5'-triphosphate + D-fructose 6-phosphate | - |
Gallus gallus | 2-amino-9-beta-D-ribofuranosylpurine 5'-diphosphate + D-fructose 1,6-bisphosphate | - |
? | |
2-amino-9-beta-D-ribofuranosylpurine 5'-triphosphate + D-fructose 6-phosphate | - |
Mus musculus | 2-amino-9-beta-D-ribofuranosylpurine 5'-diphosphate + D-fructose 1,6-bisphosphate | - |
? | |
2-amino-9-beta-D-ribofuranosylpurine 5'-triphosphate + D-fructose 6-phosphate | - |
Rattus norvegicus | 2-amino-9-beta-D-ribofuranosylpurine 5'-diphosphate + D-fructose 1,6-bisphosphate | - |
? | |
2-amino-9-beta-D-ribofuranosylpurine 5'-triphosphate + D-fructose 6-phosphate | - |
Oryctolagus cuniculus | 2-amino-9-beta-D-ribofuranosylpurine 5'-diphosphate + D-fructose 1,6-bisphosphate | - |
? | |
6-mercapto-9-beta-D-ribofuranosylpurine 5'-triphosphate + D-fructose 6-phosphate | - |
Gallus gallus | 6-mercapto-9-beta-D-ribofuranosylpurine 5'-diphosphate + D-fructose 1,6-bisphosphate | - |
? | |
6-mercapto-9-beta-D-ribofuranosylpurine 5'-triphosphate + D-fructose 6-phosphate | - |
Mus musculus | 6-mercapto-9-beta-D-ribofuranosylpurine 5'-diphosphate + D-fructose 1,6-bisphosphate | - |
? | |
6-mercapto-9-beta-D-ribofuranosylpurine 5'-triphosphate + D-fructose 6-phosphate | - |
Rattus norvegicus | 6-mercapto-9-beta-D-ribofuranosylpurine 5'-diphosphate + D-fructose 1,6-bisphosphate | - |
? | |
6-mercapto-9-beta-D-ribofuranosylpurine 5'-triphosphate + D-fructose 6-phosphate | - |
Oryctolagus cuniculus | 6-mercapto-9-beta-D-ribofuranosylpurine 5'-diphosphate + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | - |
Gallus gallus | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | - |
Lactiplantibacillus plantarum | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | - |
Mus musculus | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | - |
Thermus thermophilus | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | - |
Escherichia coli | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | - |
Homo sapiens | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | - |
Rattus norvegicus | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | - |
Saccharomyces cerevisiae | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | - |
Bos taurus | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | - |
Oryctolagus cuniculus | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | - |
Neurospora crassa | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | - |
Ovis aries | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | - |
Pisum sativum | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | - |
Zea mays | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | - |
Klebsiella aerogenes | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | - |
Dictyostelium discoideum | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | - |
Lacticaseibacillus casei | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | - |
Clostridium pasteurianum | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | - |
Daucus carota | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | - |
Glutamicibacter nicotianae | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | - |
Fasciola hepatica | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | - |
Arthrobacter crystallopoietes | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | - |
Brassica oleracea var. gemmifera | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | poor substrates are fructose 1-phosphate, glucose 1-phosphate and sedoheptulose 7-phosphate | Gallus gallus | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | poor substrates are fructose 1-phosphate, glucose 1-phosphate and sedoheptulose 7-phosphate | Lactiplantibacillus plantarum | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | poor substrates are fructose 1-phosphate, glucose 1-phosphate and sedoheptulose 7-phosphate | Mus musculus | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | poor substrates are fructose 1-phosphate, glucose 1-phosphate and sedoheptulose 7-phosphate | Thermus thermophilus | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | poor substrates are fructose 1-phosphate, glucose 1-phosphate and sedoheptulose 7-phosphate | Escherichia coli | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | poor substrates are fructose 1-phosphate, glucose 1-phosphate and sedoheptulose 7-phosphate | Homo sapiens | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | poor substrates are fructose 1-phosphate, glucose 1-phosphate and sedoheptulose 7-phosphate | Rattus norvegicus | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | poor substrates are fructose 1-phosphate, glucose 1-phosphate and sedoheptulose 7-phosphate | Saccharomyces cerevisiae | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | poor substrates are fructose 1-phosphate, glucose 1-phosphate and sedoheptulose 7-phosphate | Bos taurus | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | poor substrates are fructose 1-phosphate, glucose 1-phosphate and sedoheptulose 7-phosphate | Oryctolagus cuniculus | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | poor substrates are fructose 1-phosphate, glucose 1-phosphate and sedoheptulose 7-phosphate | Neurospora crassa | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | poor substrates are fructose 1-phosphate, glucose 1-phosphate and sedoheptulose 7-phosphate | Ovis aries | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | poor substrates are fructose 1-phosphate, glucose 1-phosphate and sedoheptulose 7-phosphate | Pisum sativum | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | poor substrates are fructose 1-phosphate, glucose 1-phosphate and sedoheptulose 7-phosphate | Zea mays | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | poor substrates are fructose 1-phosphate, glucose 1-phosphate and sedoheptulose 7-phosphate | Klebsiella aerogenes | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | poor substrates are fructose 1-phosphate, glucose 1-phosphate and sedoheptulose 7-phosphate | Dictyostelium discoideum | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | poor substrates are fructose 1-phosphate, glucose 1-phosphate and sedoheptulose 7-phosphate | Lacticaseibacillus casei | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | poor substrates are fructose 1-phosphate, glucose 1-phosphate and sedoheptulose 7-phosphate | Clostridium pasteurianum | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | poor substrates are fructose 1-phosphate, glucose 1-phosphate and sedoheptulose 7-phosphate | Daucus carota | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | poor substrates are fructose 1-phosphate, glucose 1-phosphate and sedoheptulose 7-phosphate | Glutamicibacter nicotianae | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | poor substrates are fructose 1-phosphate, glucose 1-phosphate and sedoheptulose 7-phosphate | Fasciola hepatica | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | poor substrates are fructose 1-phosphate, glucose 1-phosphate and sedoheptulose 7-phosphate | Arthrobacter crystallopoietes | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | poor substrates are fructose 1-phosphate, glucose 1-phosphate and sedoheptulose 7-phosphate | Brassica oleracea var. gemmifera | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | purine NTPs preferred | Brassica oleracea var. gemmifera | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + fructose 1-phosphate | - |
Escherichia coli | ADP + fructose 1,6-bisphosphate | - |
? | |
ATP + fructose 1-phosphate | 5% of activity with D-fructose 6-phosphate | Oryctolagus cuniculus | ADP + fructose 1,6-bisphosphate | - |
? | |
CTP + D-fructose 6-phosphate | - |
Escherichia coli | CDP + D-fructose 1,6-bisphosphate | - |
? | |
CTP + D-fructose 6-phosphate | - |
Saccharomyces cerevisiae | CDP + D-fructose 1,6-bisphosphate | - |
? | |
CTP + D-fructose 6-phosphate | - |
Oryctolagus cuniculus | CDP + D-fructose 1,6-bisphosphate | - |
? | |
CTP + D-fructose 6-phosphate | - |
Clostridium pasteurianum | CDP + D-fructose 1,6-bisphosphate | - |
? | |
CTP + D-fructose 6-phosphate | purine NTPs preferred | Brassica oleracea var. gemmifera | CDP + D-fructose 1,6-bisphosphate | - |
? | |
dATP + D-fructose 6-phosphate | - |
Escherichia coli | dADP + D-fructose 1,6-bisphosphate | - |
? | |
GTP + D-fructose 6-phosphate | - |
Escherichia coli | GDP + D-fructose 1,6-bisphosphate | - |
? | |
GTP + D-fructose 6-phosphate | - |
Saccharomyces cerevisiae | GDP + D-fructose 1,6-bisphosphate | - |
? | |
GTP + D-fructose 6-phosphate | - |
Oryctolagus cuniculus | GDP + D-fructose 1,6-bisphosphate | - |
? | |
GTP + D-fructose 6-phosphate | - |
Clostridium pasteurianum | GDP + D-fructose 1,6-bisphosphate | - |
? | |
GTP + D-fructose 6-phosphate | purine NTPs preferred | Brassica oleracea var. gemmifera | GDP + D-fructose 1,6-bisphosphate | - |
? | |
ITP + D-fructose 6-phosphate | - |
Escherichia coli | IDP + D-fructose 1,6-bisphosphate | - |
? | |
ITP + D-fructose 6-phosphate | - |
Saccharomyces cerevisiae | IDP + D-fructose 1,6-bisphosphate | - |
? | |
ITP + D-fructose 6-phosphate | - |
Oryctolagus cuniculus | IDP + D-fructose 1,6-bisphosphate | - |
? | |
ITP + D-fructose 6-phosphate | - |
Clostridium pasteurianum | IDP + D-fructose 1,6-bisphosphate | - |
? | |
ITP + D-fructose 6-phosphate | purine NTPs preferred | Brassica oleracea var. gemmifera | IDP + D-fructose 1,6-bisphosphate | - |
? | |
TTP + D-fructose 6-phosphate | purine NTPs preferred | Escherichia coli | TDP + D-fructose 1,6-bisphosphate | - |
? | |
TTP + D-fructose 6-phosphate | purine NTPs preferred | Brassica oleracea var. gemmifera | TDP + D-fructose 1,6-bisphosphate | - |
? | |
UTP + D-fructose 6-phosphate | - |
Escherichia coli | UDP + D-fructose 1,6-bisphosphate | - |
? | |
UTP + D-fructose 6-phosphate | - |
Saccharomyces cerevisiae | UDP + D-fructose 1,6-bisphosphate | - |
? | |
UTP + D-fructose 6-phosphate | - |
Oryctolagus cuniculus | UDP + D-fructose 1,6-bisphosphate | - |
? | |
UTP + D-fructose 6-phosphate | - |
Clostridium pasteurianum | UDP + D-fructose 1,6-bisphosphate | - |
? | |
UTP + D-fructose 6-phosphate | purine NTPs preferred | Brassica oleracea var. gemmifera | UDP + D-fructose 1,6-bisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | - |
Mus musculus |
? | - |
Homo sapiens |
? | x * 36500, sedimentation equilibrium centrifugation in 6 M guanidine | Escherichia coli |
hexamer | 6 * 67000, muscle enzyme, sedimentation equilibrium ultracentrifugation or gel filtration in 6.5 M guanidine | Oryctolagus cuniculus |
tetramer | - |
Rattus norvegicus |
tetramer | - |
Clostridium pasteurianum |
tetramer | 4 * 35000, isoenzyme PFK1, tetrahedral arranged subunits, SDS-PAGE | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
- |
liver enzyme, 2 h, 80% loss of activity by dissociation of enzyme | Gallus gallus |
8 | - |
liver enzyme, 2 h, 60% loss of activity by dissociation of enzyme | Gallus gallus |
40 | 60 | 30 min, stable | Gallus gallus |
80 | - |
2 h | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
additional information | - |
pH-optimum depends on nucleoside triphosphate substrate | Saccharomyces cerevisiae |
7.6 | - |
- |
Fasciola hepatica |
7.6 | - |
ITP, optima at pH 7.6 and pH 8.2 | Saccharomyces cerevisiae |
7.8 | - |
with ATP | Saccharomyces cerevisiae |
8.2 | - |
ITP, optima at pH 7.6 and pH 8.2 | Saccharomyces cerevisiae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
heart enzyme, mild acidic conditions lead to reversible dissociation and decrease of activity, ATP or fructose 1,6-bisphosphate protects | Ovis aries |
8 | - |
- |
Oryctolagus cuniculus |
8 | - |
relatively stable to inactivation by dilution, muscle enzyme | Ovis aries |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
citrate | brain PFK | Rattus norvegicus | |
0.1 | - |
cis-aconitate | brain PFK | Rattus norvegicus | |
0.2 | - |
isocitrate | brain PFK | Rattus norvegicus | |
0.6 | - |
malate | brain PFK | Rattus norvegicus | |
1.5 | - |
succinate | brain PFK | Rattus norvegicus | |
2.5 | - |
ATP | pH 7.1 | Oryctolagus cuniculus | |
2.5 | - |
alpha-ketoglutarate | brain PFK | Rattus norvegicus |