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Literature summary for 2.7.1.11 extracted from
- Characterization of ATP-dependnet fructose 6-phosphate 1-phosphotransferase isozymes from leaf and endosperm tissues of Ricinus communis (1990), Plant Physiol., 92, 155-159.
General Stability
| General Stability | Organism |
|---|---|
| cytosolic enzymes are less stable than plastidic | Ricinus communis |
| fructose 6-phosphate, MgCl2 and ethylene glycol stabilize | Ricinus communis |
| leaf cytosolic isozyme rather unstable upon purification | Ricinus communis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-phosphoglycerate | 0.9 mM, 50% inhibition of plastid PFK | Ricinus communis |  |
| ATP | inhibition of plastid PFK above 5 mM at pH 8.0 and above 0.5 mM at pH 7.2 | Ricinus communis | |
| phosphoenolpyruvate | 0.05 mM, 50% inhibition of plastid PFK | Ricinus communis | |
| Phosphoglycolate | 0.25 mM, 50% inhibition of plastid PFK | Ricinus communis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic study | Ricinus communis | |
| 0.0087 | - |
ATP | pH 7.0, 25°C, cytosolic PFK | Ricinus communis | |
| 0.013 | - |
ATP | pH 7.0, 25°C, chloroplasr PFK | Ricinus communis | |
| 0.016 | - |
fructose 6-phosphate | pH 7.0, 25°C, cytosolic PFK | Ricinus communis | |
| 0.028 | - |
ATP | pH 8.0, 25°C, cytosolic PFK | Ricinus communis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | cytosolic and plastidic isoenzyme | Ricinus communis | 5829 | - |
| plastid | leukoplasts | Ricinus communis | 9536 | - |
| plastid | chloroplasts | Ricinus communis | 9536 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for activity | Ricinus communis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
4 * 57000, leukoplast PFK, SDS-PAGE | Ricinus communis |
| 220000 | - |
leukoplast PFK, gel filtration | Ricinus communis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ricinus communis | - |
castor bean, cv. Baker 296, dwarf, hybrid, plastid and cytosolic isoenzymes | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| leukoplast PFK, acetone powder, DEAE-Sephacel, Mono Q, Phenyl-Sepharose | Ricinus communis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| endosperm | - |
Ricinus communis | - |
| leaf | - |
Ricinus communis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 48 | - |
leucoplast PFK | Ricinus communis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, frozen with liquid nitrogen, several months, no loss of leukoplast PFK activity | Ricinus communis |
| 4°C, 70% loss of activity within 20 h, leaf cytosolic isozyme, 5 mM MgCl2 and 20% v/v ethylene glycol stabilizes | Ricinus communis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose 6-phosphate | - |
Ricinus communis | ADP + D-fructose 1,6-bisphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 57000, leukoplast PFK, SDS-PAGE | Ricinus communis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.7 | 8.3 | endosperm | Ricinus communis |
| 8 | - |
plastid isoenzyme | Ricinus communis |
| 8.5 | 9 | cytosolic isoenzyme from leaf | Ricinus communis |
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