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Literature summary for 2.7.1.11 extracted from

  • Kruger, N.J.; Hammond, J.B.W.; Burrell, M.M.
    Molecular characterization of four forms of phosphofructokinase purified from potato tuber (1988), Arch. Biochem. Biophys., 267, 690-700.
    View publication on PubMed
Search for more literature for 2.7.1.11

General Stability

General Stability Organism
glycerol and KCl or ATP stabilize Escherichia coli
glycerol and KCl or ATP stabilize Solanum tuberosum
glycerol and KCl or ATP stabilize Solanum acaule
glycerol and KCl or ATP stabilize Solanum commersonii
glycerol and KCl or ATP stabilize Solanum megistacrolobum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Escherichia coli
Mg2+ required for activity Solanum tuberosum
Mg2+ required for activity Solanum acaule
Mg2+ required for activity Solanum commersonii
Mg2+ required for activity Solanum megistacrolobum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46300
-
 x * 46300 + x * 49500 + x * 50000 + x * 53000, 4 enzyme forms of different subunit composition, SDS-PAGE with 4 M urea Solanum tuberosum
49500
-
 x * 46300 + x * 49500 + x * 50000 + x * 53000, 4 enzyme forms of different subunit composition, SDS-PAGE with 4 M urea Solanum tuberosum
50000
-
 x * 46300 + x * 49500 + x * 50000 + x * 53000, 4 enzyme forms of different subunit composition, SDS-PAGE with 4 M urea Solanum tuberosum
53000
-
 x * 46300 + x * 49500 + x * 50000 + x * 53000, 4 enzyme forms of different subunit composition, SDS-PAGE with 4 M urea Solanum tuberosum
196000
-
 isoform I, gel filtration Solanum tuberosum
201000
-
 isoform II, gel filtration Solanum tuberosum
203000
-
 isoform III, gel filtration Solanum tuberosum
800000
-
 isoform IV, gel filtration Solanum tuberosum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-fructose 6-phosphate Escherichia coli first step in glycolysis ADP + D-fructose 1,6-bisphosphate
-
 ?
ATP + D-fructose 6-phosphate Solanum tuberosum first step in glycolysis ADP + D-fructose 1,6-bisphosphate
-
 ?
ATP + D-fructose 6-phosphate Solanum acaule first step in glycolysis ADP + D-fructose 1,6-bisphosphate
-
 ?
ATP + D-fructose 6-phosphate Solanum commersonii first step in glycolysis ADP + D-fructose 1,6-bisphosphate
-
 ?
ATP + D-fructose 6-phosphate Solanum megistacrolobum first step in glycolysis ADP + D-fructose 1,6-bisphosphate
-
 ?
ATP + D-fructose 6-phosphate Escherichia coli DF1020 first step in glycolysis ADP + D-fructose 1,6-bisphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 containing overproducing plasmid pHE1007
-
Escherichia coli DF1020
-
 containing overproducing plasmid pHE1007
-
Solanum acaule
-
-
-
Solanum commersonii
-
 wild potato species
-
Solanum megistacrolobum
-
-
-
Solanum tuberosum
-
 potato, cv. Record, Hertha or Cara, isoforms I-IV
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli
isoforms I-IV, DEAE-Sephacel, Red-agarose, ATP-agarose, Mono Q Solanum tuberosum

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Solanum tuberosum
-
leaf
-
 Solanum acaule
-
leaf
-
 Solanum commersonii
-
leaf
-
 Solanum megistacrolobum
-
tuber
-
 Solanum tuberosum
-
tuber
-
 Solanum commersonii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
189 215
-
 Escherichia coli
200
-
 isoforms I-IV Solanum tuberosum

Storage Stability

Storage Stability Organism
-20°C, 0.1 M Tris-HCl buffer, pH 8, 2 mM MgCl2, 1 mM EDTA, 14 mM 2-mercaptoethanol, 10% v/v glycerol, 20% loss of activity within 3 weeks Escherichia coli
-20°C, 0.1 M Tris-HCl buffer, pH 8, 2 mM MgCl2, 1 mM EDTA, 14 mM 2-mercaptoethanol, 10% v/v glycerol, 20% loss of activity within 3 weeks Solanum tuberosum
-20°C, 0.1 M Tris-HCl buffer, pH 8, 2 mM MgCl2, 1 mM EDTA, 14 mM 2-mercaptoethanol, 10% v/v glycerol, 20% loss of activity within 3 weeks Solanum acaule
-20°C, 0.1 M Tris-HCl buffer, pH 8, 2 mM MgCl2, 1 mM EDTA, 14 mM 2-mercaptoethanol, 10% v/v glycerol, 20% loss of activity within 3 weeks Solanum commersonii
-20°C, 0.1 M Tris-HCl buffer, pH 8, 2 mM MgCl2, 1 mM EDTA, 14 mM 2-mercaptoethanol, 10% v/v glycerol, 20% loss of activity within 3 weeks Solanum megistacrolobum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 6-phosphate
-
 Escherichia coli ADP + D-fructose 1,6-bisphosphate
-
 ?
ATP + D-fructose 6-phosphate
-
 Solanum tuberosum ADP + D-fructose 1,6-bisphosphate
-
 ?
ATP + D-fructose 6-phosphate
-
 Solanum acaule ADP + D-fructose 1,6-bisphosphate
-
 ?
ATP + D-fructose 6-phosphate
-
 Solanum commersonii ADP + D-fructose 1,6-bisphosphate
-
 ?
ATP + D-fructose 6-phosphate
-
 Solanum megistacrolobum ADP + D-fructose 1,6-bisphosphate
-
 ?
ATP + D-fructose 6-phosphate first step in glycolysis Escherichia coli ADP + D-fructose 1,6-bisphosphate
-
 ?
ATP + D-fructose 6-phosphate first step in glycolysis Solanum tuberosum ADP + D-fructose 1,6-bisphosphate
-
 ?
ATP + D-fructose 6-phosphate first step in glycolysis Solanum acaule ADP + D-fructose 1,6-bisphosphate
-
 ?
ATP + D-fructose 6-phosphate first step in glycolysis Solanum commersonii ADP + D-fructose 1,6-bisphosphate
-
 ?
ATP + D-fructose 6-phosphate first step in glycolysis Solanum megistacrolobum ADP + D-fructose 1,6-bisphosphate
-
 ?
ATP + D-fructose 6-phosphate
-
 Escherichia coli DF1020 ADP + D-fructose 1,6-bisphosphate
-
 ?
ATP + D-fructose 6-phosphate first step in glycolysis Escherichia coli DF1020 ADP + D-fructose 1,6-bisphosphate
-
 ?

Subunits

Subunits Comment Organism
oligomer
-
 Escherichia coli
oligomer
-
 Solanum acaule
oligomer
-
 Solanum commersonii
oligomer
-
 Solanum megistacrolobum
oligomer x * 46300 + x * 49500 + x * 50000 + x * 53000, 4 enzyme forms of different subunit composition, SDS-PAGE with 4 M urea Solanum tuberosum