Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ADP | activation | Aspergillus niger | |
ADP | at high fructose 6-phosphate concentration, inhibits at low fructose 6-phosphate concentration | Aspergillus niger | |
AMP | activation at low fructose 6-phosphate concentrations, inhibition at high fructose 6-phosphate concentrations | Aspergillus niger | |
D-glucose 6-phosphate | 4.8 mM, 1.5fold increase in activity at 2 mM fructose 6-phosphate, 2fold increase at 4 mM fructose 6-phosphate, in vitro | Aspergillus niger | |
additional information | not activated by 2-oxoglutarate | Aspergillus niger | |
NH4+ | NH4+ increases the affinity for fructose 6-phosphate, counteracts the inhibition by ATP | Aspergillus niger |
General Stability | Organism |
---|---|
dilution inactivates | Aspergillus niger |
fructose 6-phosphate stabilizes, not 1 mM ATP or 50% v/v glycerol | Aspergillus niger |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | kinetics | Aspergillus niger | |
AMP | at high fructose 6-phosphate concentrations, activates at low fructose 6-phosphate concentrations | Aspergillus niger | |
ATP | synergistic with citrate | Aspergillus niger | |
citrate | strong inhibition; synergistic with ATP; synergistic with NH4+; synergistic with phosphate and AMP | Aspergillus niger | |
additional information | not inhibited by cAMP | Aspergillus niger | |
phosphoenolpyruvate | - |
Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
ATP | pH 7.8, 30°C | Aspergillus niger | |
0.08 | - |
GTP | pH 7.8, 30°C | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Aspergillus niger B60 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Aspergillus niger |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Aspergillus niger | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.1 | - |
- |
Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | - |
Aspergillus niger | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | - |
Aspergillus niger B60 | ADP + D-fructose 1,6-bisphosphate | - |
? | |
GTP + D-fructose 6-phosphate | - |
Aspergillus niger | GDP + D-fructose 1,6-bisphosphate | - |
? | |
GTP + D-fructose 6-phosphate | - |
Aspergillus niger B60 | GDP + D-fructose 1,6-bisphosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | 8 | - |
Aspergillus niger |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
phosphoenolpyruvate | pH 7.8, 30°C | Aspergillus niger | |
0.25 | - |
citrate | pH 7.8, 30°C, at 0.1 mM ATP | Aspergillus niger | |
0.4 | - |
citrate | pH 7.8, 30°C, at 0.5 mM ATP | Aspergillus niger |