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Literature summary for 2.7.1.11 extracted from

  • Kemp, R.G.
    Phosphofructokinase from rabbit liver (1975), Methods Enzymol., 42C, 67-71.
    View publication on PubMed
Search for more literature for 2.7.1.11

Activating Compound

Activating Compound Comment Organism Structure
NH4+
-
 Oryctolagus cuniculus

General Stability

General Stability Organism
fructose 6-phosphate, fructose 1,6-bisphosphate and ammonium sulfate stabilize dilute enzyme solutions Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
2,3-diphosphoglycerate
-
 Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
liver PFK, ammonium sulfate, DEAE-Sephadex, agarose chromatography Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
85 95
-
 Oryctolagus cuniculus

Storage Stability

Storage Stability Organism
-20°C, more than 2 mg protein per ml, in 50 mM Tris-phosphate buffer, pH 8, 2 mM EDTA, 100 mM ammonium sulfate, 0.1 mM ATP, 0.5 mM dithiothreitol, several months Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 6-phosphate
-
 Oryctolagus cuniculus ADP + D-fructose 1,6-bisphosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
26
-
 assay at Oryctolagus cuniculus