2.5.1.96: 4,4'-diapophytoene synthase

This is an abbreviated version!
For detailed information about 4,4'-diapophytoene synthase, go to the full flat file.

Word Map on EC 2.5.1.96

2.5.1.96

staphylococcus

aureus

carotenoid

staphyloxanthin

desaturase

head-to-head

4,4'-diaponeurosporene

allyl

carbocation

desaturation

dimethylallyl

head-to-tail

synthesis

Reaction

2 (2E,6E)-farnesyl diphosphate =

+ 2 diphosphate

Synonyms

CrtM, DAP synthase, dehydrosqualene synthase, diapophytoene synthase, DSQ synthase, DSQS

ECTree

2 Transferases

2.5 Transferring alkyl or aryl groups, other than methyl groups

2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)

2.5.1.96 4,4'-diapophytoene synthase

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Results	in table
425	AA Sequence
1	Application
12	Cloned(Commentary)
5	Crystallization (Commentary)
9	General Information
10	IC50 Value
20	Inhibitors
7	Ki Value [mM]
1	KM Value [mM]
1	Metals/Ions
2	Molecular Weight [Da]
14	Natural Substrates/ Products (Substrates)
24	Organism
4	Pathway
10	PDB ID
22	Protein Variants
2	Purification (Commentary)
4	Reaction
2	Reaction Type
23	Reference
19	Substrates and Products (Substrate)
3	Subunits
22	Synonyms
1	Systematic Name

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Cloned on EC 2.5.1.96 - 4,4'-diapophytoene synthase

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4 entries

expressed in Escherichia coli Rosetta(DE3) cells

Staphylococcus aureus

758820

expression in Escherichia coli

2 entries

gene crtM, DNA and amino acid sequence determination and analysis, genetic organization, sequence comparisons, phylogenetic tree, functional pathway complementation by recombinant enzyme expression in Escherichia coli

2 entries

gene ysqs, recombinant expression of the engineered mutant enzyme in Escherichia coli strain XL1-Blue, coexpression with gene crtN encoding DSQ desaturase from Staphylococcus aureus in Escherichia coli results in carotinoid production and accumulation of C30 carotenoid pigments, which does not happen with coexpression of gene crtI encoding Pantoea ananatis phytoene desaturase, carotenoid pigment analysis, overview

Saccharomyces cerevisiae

738235

recombinant expression of the endgineered enzymes in Escherichia coli strain XL1-Blue, coexpression with gene crtN encoding DSQ desaturase from Staphylococcus aureus in Escherichia coli results in carotinoid production and accumulation of C30 carotenoid pigments, which does not happen with coexpression of gene crtI encoding Pantoea ananatis phytoene desaturase, carotenoid pigment analysis, overview

Homo sapiens

738235

recombinant expression of the engineered mutant enzyme in Escherichia coli strain XL1-Blue, coexpression with gene crtN encoding DSQ desaturase from Staphylococcus aureus in Escherichia coli results in carotinoid production and accumulation of C30 carotenoid pigments, which does not happen with coexpression of gene crtI encoding Pantoea ananatis phytoene desaturase, carotenoid pigment analysis, overview

Thermosynechococcus vestitus

738235