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Literature summary for 2.5.1.96 extracted from
- Directed evolution of squalene synthase for dehydrosqualene biosynthesis (2014), FEBS Lett., 588, 3375-3381.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene ysqs, recombinant expression of the engineered mutant enzyme in Escherichia coli strain XL1-Blue, coexpression with gene crtN encoding DSQ desaturase from Staphylococcus aureus in Escherichia coli results in carotinoid production and accumulation of C30 carotenoid pigments, which does not happen with coexpression of gene crtI encoding Pantoea ananatis phytoene desaturase, carotenoid pigment analysis, overview | Saccharomyces cerevisiae |
| recombinant expression of the endgineered enzymes in Escherichia coli strain XL1-Blue, coexpression with gene crtN encoding DSQ desaturase from Staphylococcus aureus in Escherichia coli results in carotinoid production and accumulation of C30 carotenoid pigments, which does not happen with coexpression of gene crtI encoding Pantoea ananatis phytoene desaturase, carotenoid pigment analysis, overview | Homo sapiens |
| recombinant expression of the engineered mutant enzyme in Escherichia coli strain XL1-Blue, coexpression with gene crtN encoding DSQ desaturase from Staphylococcus aureus in Escherichia coli results in carotinoid production and accumulation of C30 carotenoid pigments, which does not happen with coexpression of gene crtI encoding Pantoea ananatis phytoene desaturase, carotenoid pigment analysis, overview | Thermosynechococcus vestitus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| G856A/G1125A/T1128C/T1176C | directed evolution of squalene synthase, EC 2.5.1.21, in an attempt to mimic the activity of dehydroqsqualene synthase, EC 2.5.1.96, by mutation of gene ysqs | Saccharomyces cerevisiae |
| additional information | directed evolution of squalene synthase, EC 2.5.1.21, in an attempt to mimic the activity of dehydroqsqualene synthase, EC 2.5.1.96, by mutation gene tsqs replacement with the human gene mutant Q33R/D34N/S38N/G856A/G1125A/T1128C/T1176C | Thermosynechococcus vestitus |
| Q33R/D34N/S38N | directed evolution of human Q33R/D34N/S38N mutant squalene synthase, EC 2.5.1.21, in an attempt to mimic the activity of dehydroqsqualene synthase, EC 2.5.1.96, by replacement of hsqs in pAC-hsqs with the Saccharomyces mutant gene ysqs G856A/G1125A/T1128C/T1176C | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 (2E,6E)-farnesyl diphosphate | Homo sapiens | - |
15-cis-4,4'-diapophytoene + 2 diphosphate | - |
? |  |
| 2 (2E,6E)-farnesyl diphosphate | Saccharomyces cerevisiae | - |
15-cis-4,4'-diapophytoene + 2 diphosphate | - |
? | |
| 2 (2E,6E)-farnesyl diphosphate | Thermosynechococcus vestitus | - |
15-cis-4,4'-diapophytoene + 2 diphosphate | - |
? | |
| additional information | Homo sapiens | reaction mechanism of squalene and dehydrosqualene formation and their conversion into carotenoid pigments, overview | ? | - |
? | |
| additional information | Saccharomyces cerevisiae | reaction mechanism of squalene and dehydrosqualene formation and their conversion into carotenoid pigments, overview | ? | - |
? | |
| additional information | Thermosynechococcus vestitus | reaction mechanism of squalene and dehydrosqualene formation and their conversion into carotenoid pigments, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Saccharomyces cerevisiae | - |
- |
- |
| Thermosynechococcus vestitus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 (2E,6E)-farnesyl diphosphate | - |
Homo sapiens | 15-cis-4,4'-diapophytoene + 2 diphosphate | - |
? | |
| 2 (2E,6E)-farnesyl diphosphate | - |
Saccharomyces cerevisiae | 15-cis-4,4'-diapophytoene + 2 diphosphate | - |
? | |
| 2 (2E,6E)-farnesyl diphosphate | - |
Thermosynechococcus vestitus | 15-cis-4,4'-diapophytoene + 2 diphosphate | - |
? | |
| additional information | reaction mechanism of squalene and dehydrosqualene formation and their conversion into carotenoid pigments, overview | Homo sapiens | ? | - |
? | |
| additional information | reaction mechanism of squalene and dehydrosqualene formation and their conversion into carotenoid pigments, overview | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | reaction mechanism of squalene and dehydrosqualene formation and their conversion into carotenoid pigments, overview | Thermosynechococcus vestitus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dehydrosqualene synthase | - |
Homo sapiens |
| dehydrosqualene synthase | - |
Saccharomyces cerevisiae |
| dehydrosqualene synthase | - |
Thermosynechococcus vestitus |
| DSQ synthase | - |
Homo sapiens |
| DSQ synthase | - |
Saccharomyces cerevisiae |
| DSQ synthase | - |
Thermosynechococcus vestitus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | a mutant of squalene synthase is converted to a dehydrosqualene synthase, the various mutations are lustered around the residues that are proposed to be important for NADPH binding | Homo sapiens |
| malfunction | a mutant of squalene synthase is converted to a dehydrosqualene synthase, the various mutations are lustered around the residues that are proposed to be important for NADPH binding | Saccharomyces cerevisiae |
| malfunction | a mutant of squalene synthase is converted to a dehydrosqualene synthase, the various mutations are lustered around the residues that are proposed to be important for NADPH binding | Thermosynechococcus vestitus |
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