Literature summary for 2.5.1.96 extracted from

Ku, B.; Jeong, J.C.; Mijts, B.N.; Schmidt-Dannert, C.; Dordick, J.S.
Preparation, characterization, and optimization of an in vitro C30 carotenoid pathway (2005), Appl. Environ. Microbiol., 71, 6578-6583.

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Application

Application	Comment	Organism
synthesis	construction of an in vitro C30 carotenoid pathway based on recombinant Escherichia coli farnesyl diphosphate synthase and 4,4'-diapophytoene synthase. The 4,4'-diapophytoene product is unstabel in aqueous buffer. In situ extraction using an aqueous-organic two-phase system results in a 100% conversion of isopentenyl diphosphate and dimethylallyl diphosphate into 4,4'-diapophytoene synthase	Staphylococcus aureus

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Staphylococcus aureus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0012	-	(2E,6E)-farnesyl diphosphate	pH 7.7, 37°C	Staphylococcus aureus

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus aureus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein	Staphylococcus aureus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 (2E,6E)-farnesyl diphosphate	-	Staphylococcus aureus	cis-4,4'-diapophytoene + 2 diphosphate	-	?

Synonyms

Synonyms	Comment	Organism
DAP synthase	-	Staphylococcus aureus

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Release 2023.1 (January 2023)