2.5.1.96: 4,4'-diapophytoene synthase
This is an abbreviated version!
For detailed information about 4,4'-diapophytoene synthase, go to the full flat file.
Word Map on EC 2.5.1.96
-
2.5.1.96
-
staphylococcus
-
aureus
-
carotenoid
-
staphyloxanthin
-
desaturase
-
head-to-head
-
4,4'-diaponeurosporene
-
allyl
-
carbocation
-
desaturation
-
dimethylallyl
-
head-to-tail
-
synthesis
- 2.5.1.96
- staphylococcus
- aureus
-
carotenoid
- staphyloxanthin
-
desaturase
-
head-to-head
- 4,4'-diaponeurosporene
-
allyl
-
carbocation
-
desaturation
-
dimethylallyl
-
head-to-tail
- synthesis
Reaction
2 (2E,6E)-farnesyl diphosphate = + 2 diphosphate
Synonyms
CrtM, DAP synthase, dehydrosqualene synthase, diapophytoene synthase, DSQ synthase, DSQS
ECTree
Advanced search results
Engineering
Engineering on EC 2.5.1.96 - 4,4'-diapophytoene synthase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Q33R/D34N/S38N
-
directed evolution of human Q33R/D34N/S38N mutant squalene synthase, EC 2.5.1.21, in an attempt to mimic the activity of dehydroqsqualene synthase, EC 2.5.1.96, by replacement of hsqs in pAC-hsqs with the Saccharomyces mutant gene ysqs G856A/G1125A/T1128C/T1176C
G856A/G1125A/T1128C/T1176C
-
directed evolution of squalene synthase, EC 2.5.1.21, in an attempt to mimic the activity of dehydroqsqualene synthase, EC 2.5.1.96, by mutation of gene ysqs
A726G, A850G
-
mutant with phytoene synthase activity, decrease in dehydrosqualene synthase activity
F26L
-
mutation is sufficient to gain phytoene synthase activity, decrease in dehydrosqualene synthase activity
F26L/E149G
-
mutant with phytoene synthase activity, decrease in dehydrosqualene synthase activity
F26L/F267S
-
mutant with phytoene synthase activity, decrease in dehydrosqualene synthase activity
F26L/V43M
-
mutant with phytoene synthase activity, decrease in dehydrosqualene synthase activity
F26S/I40T
-
mutant with phytoene synthase activity, decrease in dehydrosqualene synthase activity
H12R/F26L/D27G/90G/K97R/H207R
-
mutant with phytoene synthase activity, decrease in dehydrosqualene synthase activity
K20E/F26S
-
mutant with phytoene synthase activity, decrease in dehydrosqualene synthase activity
M4V/H12R/F59S/Q81R/E180G
-
mutant with phytoene synthase activity, without decrease in dehydrosqualene synthase activity
Y129A
no activity with substrate farnesyl diphophate, 7% residual activity with geranyl diphosphate
F26L
-
mutation is sufficient to gain phytoene synthase activity, decrease in dehydrosqualene synthase activity
-
F26L/E149G
-
mutant with phytoene synthase activity, decrease in dehydrosqualene synthase activity
-
F26L/V43M
-
mutant with phytoene synthase activity, decrease in dehydrosqualene synthase activity
-
K20E/F26S
-
mutant with phytoene synthase activity, decrease in dehydrosqualene synthase activity
-
additional information
expression of CrtM in Escherichia coli leads to synthesis of diapophytoene from pre-existing farnesyl diphosphate
additional information
-
expression of CrtM in Escherichia coli leads to synthesis of diapophytoene from pre-existing farnesyl diphosphate
additional information
-
expression of CrtM in Escherichia coli leads to synthesis of diapophytoene from pre-existing farnesyl diphosphate
-
additional information
-
construction of C40 carotenoid synthase pathway in Escherichia coli by expression of crtE encoding GGPP synthase, crtB encoding phytoene synthase, and crtI encoding phytoene desaturase, all from Erwinia uredovora. Replacement of phytoene synthase crtB by dehydrosqualene synthase crtM results in negligible ability to synthesize the C40 product. Some crtM mutants perform comparably to crtB in an in vivo C40 pathway. These mutants show significant variation in performance in their original C30 pathway, indicating the emergence of enzymes with broadened substrate specificity as well as those with shifted specificity
additional information
-
construction of C40 carotenoid synthase pathway in Escherichia coli by expression of crtE encoding GGPP synthase, crtB encoding phytoene synthase, and crtI encoding phytoene desaturase, all from Erwinia uredovora. Replacement of phytoene synthase crtB by dehydrosqualene synthase crtM results in negligible ability to synthesize the C40 product. Some crtM mutants perform comparably to crtB in an in vivo C40 pathway. These mutants show significant variation in performance in their original C30 pathway, indicating the emergence of enzymes with broadened substrate specificity as well as those with shifted specificity
-
additional information
Thermosynechococcus vestitus
-
directed evolution of squalene synthase, EC 2.5.1.21, in an attempt to mimic the activity of dehydroqsqualene synthase, EC 2.5.1.96, by mutation gene tsqs replacement with the human gene mutant Q33R/D34N/S38N/G856A/G1125A/T1128C/T1176C