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2.5.1.19: 3-phosphoshikimate 1-carboxyvinyltransferase

This is an abbreviated version!
For detailed information about 3-phosphoshikimate 1-carboxyvinyltransferase, go to the full flat file.

Word Map on EC 2.5.1.19

Reaction

phosphoenolpyruvate
+
3-phosphoshikimate
=
phosphate
 +
5-O-(1-carboxyvinyl)-3-phosphoshikimate

Synonyms

3-enolpyruvylshikimate 5-phosphate synthase, 3-enolpyruvylshikimic acid-5-phosphate synthetase, 3-phosphoshikimate 1-carboxyvinyl-transferase, 3-phosphoshikimate 1-carboxyvinyltransferase, 3-phosphoshikimate-1-carboxyvinyl-transferase, 5'-enolpyruvylshikimate-3-phosphate synthase, 5-enol pyruvylshikimate 3-phosphate synthase, 5-enol-pyruvyl-shikimate-3-phosphate synthase, 5-enol-pyruvylshikimate 3-phosphate synthase, 5-enol-pyruvylshikimate-3-phosphate synthase, 5-enolpyruvyl-3-phosphoshikimate synthase, 5-enolpyruvylshikimate 3-phosphate synthase, 5-enolpyruvylshikimate-3-phosphate synthase, 5-enolpyruvylshikimate-3-phosphate synthetase, 5-enolpyruvylshikimate-3-phosphoric acid synthase, 5-enolpyruvylshikimate-3phosphate synthase, 5-enolpyruvylshikimic acid-3-phosphate synthase, 5-enopyruvylshikimate-3-phosphate synthase, 5-enoylpyruvylshikimate 3-phosphate synthase, 5-enoylpyruvylshikimate-3-phosphate synthase, A1501 EPSPS, AM79, AroA, AroA(1398), AroA(A1501), AroA1398, CaEPSPS, cp4 epsps, DsaroA, Dunaliella salina 5-enolpyruvylshikimate-3-phosphate synthase, Dunaliella salina EPSP synthase, E. coli EPSPS, EcaroA, enolpyruvylshikimate 3-phosphate synthase, enolpyruvylshikimate phosphate synthase, enolpyruvylshikimate-3-phosphate synthase, EPSP synhthase, EPSP synthase, EPSPS, G2 5-enolpyruvyl shikimate 3-phosphate synthase, G2 EPSPS, GR79Ms, maize EPSP synthase, maize EPSPS, mEPSPS, More, Mt EPSPS, Mycobacterium tuberculosis 5-enolpyruvylshikimate-3-phosphate synthase, Potri.002G146400, StaroA, synthase, 5-enolpyruvoylshikimate 3-phosphate

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.19 3-phosphoshikimate 1-carboxyvinyltransferase

Cloned

Cloned on EC 2.5.1.19 - 3-phosphoshikimate 1-carboxyvinyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
amplified by 3’ and 5’ RACE on total RNA according to an EST found in Dunaliella salina cDNA library, cloned into pMD18-T for sequencing, cloned in pGEX-4T-1 for expression as mature protein (lacking N-terminal 70 amino acids long transit peptide) in Escherichia coli JM109 or EPSP synthase gene deleted Escherichia coli strain ER2799
by PCR from genomic DNA of strain A1501 and error-prone PCR on full-length EPSPS gene, in pACYC184 for transformation and complementation analyses in aroA-inactive mutant Escherichia coli AKM4188, or in pET-28a for expression in Escherichia coli BL21
-
coding sequence of CaEPSPS gene is successfully subcloned in a plasmid-Escherichia coli system (pET-32a), and the cells containing the plasmid carrying the CaEPSPS gene exhibit enhanced tolerance to herbicide glyphosate, compared to the control
Escherichia coli structural gene aroA
-
expressed as a His-tagged fusion protein
expressed in Arabidopsis thaliana
-
expressed in Arabidopsis thaliana ecotype Columbia
expressed in Escherichia coli
-
expressed in Escherichia coli AB2829 cells
expressed in Escherichia coli BL21 (DE3) cells and in Oryza sativa
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells
-
expressed in Escherichia coli Rosetta (DE3) cells
-
expressed in Escherichia coli strain AB2829
-
expressed in Escherichia coli strain BL21 (DE3) and Arabidopsis thaliana
-
expressed in Escherichia coli strain BL21 and Isatis indigotica hairy roots
-
expressed in Escherichia coli strain TOP10 and Medicago sativa
-
expressed in Oryza sativa and Oryza rufipogon
-
expressed in Zea mays MZHG0JG
-
expression in Escherichia coli
expression of the chimeric mutant aroA-M1, comprising N-terminal part of the Escherichia coli enzyme and the C-terminal part of the enzyme from Salmonella typhimurium in Escherichia coli strain XL1-Blue
-
expression of the chimeric mutant aroA-M1, comprising N-terminal part of the Escherichia coli enzyme and the C-terminal part of the enzyme from Salmonella typhimurium, and of T42M-aroA mutant in Escherichia coli strain BL21(DE3)
-
expression of the wild-type enzyme and of the chimeric mutant aroA-M1, comprising N-terminal part of the Escherichia coli enzyme and the C-terminal part of the enzyme from Salmonella typhimurium in strain XL1-Blue
-
expression of the wild-type enzyme, of the chimeric mutant aroA-M1, comprising N-terminal part of the Escherichia coli enzyme and the C-terminal part of the enzyme from Salmonella typhimurium, and of T42M-aroA mutant in strain BL21(DE3)
-
expression of wild-type and chimeras of Salmonella and Escherichia coli mutant enzymes in Escherichia coli
-
expression of wild-type and chimeras of Salmonella typhimurium and Escherichia coli mutant enzymes in Escherichia coli
-
expression of wild-type and G96A mutant enzyme in Escherichia coli
-
expression of wild-type and R24D, P105S, H385L mutant enzymes in Escherichia coli
-
from genomic DNA, in cosmid vector for phage packaging and transfection in Escherichia coli JM101 or in vitro transposon mutagenesis and transformation in Escherichia coli XL1 Blue MRF’, in pAX306 for transformation of Escherichia coli DH5alpha and XL1 Blue MRF’, in pRSF1b for inducible expression in Escherichia coli, in pSB11 shuttle vector for integration into Agrobacterium tumefaciens LBA4404 (already harbouring pSB1) and subsequent transformation of positive Agrobacterium strains in maize embryo for generation of transgenic plants
-
from genomic DNA, in pUC18 for complementation studies in Escherichia coli strain AB2829 (aroA auxotroph), in pTrc99A for inducible expression in Escherichia coli AB 2829
-
in pET24d for expression in Escherichia coli DE3
mutant enzyme G96A/A183T is transferred to Brassica napus via Agrobacterium-mediated transformation. Transgenic plants are resistant to glyphosate at a concentration of 10 mM whereas the non-transformed control plants are unable to survive 1 mM glyphosate
-
mutant G96A is expressed in Escherichia coli BL21 cells and Oryza sativa
N-terminal fragment (until Phe295) of G2 EPSPS or the complete gene in pMEB2 (N-terminal of splicing domain of of DnaE intein, strain PCC6803) and C-terminus (from Thr296) of G2 EPSPS in pKEB12 (C-terminal of splicing domain of DnaE intein, strain PCC6803) for intein-mediated protein complementation and expression in Escherichia coli strain ER2799, complete G2 EPSPS gene, N-terminal and C-terminal fragments in pBI121 for introduction into Agrobacterium sp. strain LBA4404 and transformation in Nicotiana tabacum variant NC89 for generation of transgenic plants
overexpressed as a His-tagged fusion protein
overexpressed in Escherichia coli
overexpression in Escherichia coli
overexpression in Escherichia coli strain BL21(DE3)
-
overexpression of wild-type and mutant enzymes in strain BL21(DE3)
overexpression of wild-type, His-tagged wild-type and several mutant enzymes in Escherichia coli
subcloned from bacteriophage lambdapserC into multicopy plasmid pAT153 with subsequent transformation of Escherichia coli AB2829 CGSC2829 cells
-
wild-type and mutants in pET-24d for expression in Escherichia coli BL21(DE3)