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0.00036 - 2
3-phosphoshikimate
0.00025 - 2.5
5-enolpyruvylshikimate 3-phosphate
0.0018 - 43.6
phosphoenolpyruvate
0.02 - 0.43
shikimate 3-phosphate
additional information
additional information
-
0.00036
3-phosphoshikimate
-
-
0.0025
3-phosphoshikimate
-
recombinant overproducing strain
0.0032 - 0.0036
3-phosphoshikimate
-
-
0.0032 - 0.0036
3-phosphoshikimate
-
wild-type enzyme
0.007
3-phosphoshikimate
-
-
0.0077
3-phosphoshikimate
-
-
0.018
3-phosphoshikimate
C-terminal His6-tagged AroA, 50 mM KCl
0.035
3-phosphoshikimate
50 mM KCl, mutant E341M
0.04
3-phosphoshikimate
50 mM KCl, mutant E341Q
0.045
3-phosphoshikimate
-
glyphosate-sensitive enzyme
0.048
3-phosphoshikimate
wild-type
0.071
3-phosphoshikimate
mutant P101S
0.077
3-phosphoshikimate
mutant P101S/T97I
0.077
3-phosphoshikimate
mutant T97I
0.08
3-phosphoshikimate
50 mM KCl, mutant E341C
0.083
3-phosphoshikimate
at pH 8.0 and 40°C
0.084
3-phosphoshikimate
mutant G96A, pH 7.0, 28°C
0.086
3-phosphoshikimate
wild-type, pH 7.0, 28°C
0.09
3-phosphoshikimate
50 mM KCl, mutant H385A
0.1
3-phosphoshikimate
50 mM KCl, mutant E341A
0.11
3-phosphoshikimate
C-terminal His6-tagged AroA, 100 mM KCl
0.111
3-phosphoshikimate
-
at pH 8.0 and 30°C
0.14
3-phosphoshikimate
-
pH 7.0, recombinant enzyme, in presence of 100 mM KCl
0.14
3-phosphoshikimate
wild-type AroA, 100 mM KCl
0.144
3-phosphoshikimate
-
at pH 7.0 and 30°C
0.169
3-phosphoshikimate
-
wild type enzyme, at pH 7.0 and 28°C
0.19
3-phosphoshikimate
50 mM KCl, mutant K22R value above 0.3
0.193
3-phosphoshikimate
-
glyphosate-resistant enzyme
0.2
3-phosphoshikimate
50 mM KCl, mutant D313N
0.21
3-phosphoshikimate
50 mM KCl, mutant D313A
0.241
3-phosphoshikimate
mutant T355S, pH 7.0, 28°C
0.275
3-phosphoshikimate
pH 7.0, 28°C
0.286
3-phosphoshikimate
wild-type, pH 7.0, 28°C
0.304
3-phosphoshikimate
pH 7.0, 28°C
0.4
3-phosphoshikimate
50 mM KCl, mutant D313C
0.7
3-phosphoshikimate
50 mM KCl, mutant D313L
0.706
3-phosphoshikimate
-
mutant enzyme G100A, at pH 7.0 and 28°C
2
3-phosphoshikimate
50 mM KCl, mutant K22A value above 0.3
0.00025
5-enolpyruvylshikimate 3-phosphate
-
-
0.001
5-enolpyruvylshikimate 3-phosphate
-
-
0.0019
5-enolpyruvylshikimate 3-phosphate
-
25°C, pH 7.0, mutant enzyme G99A
0.00195
5-enolpyruvylshikimate 3-phosphate
reverse reaction, in the presence of 50 mM KCl
0.002
5-enolpyruvylshikimate 3-phosphate
-
25°C, pH 7.0, wild-type enzyme
0.0025
5-enolpyruvylshikimate 3-phosphate
-
25°C, pH 7.0, mutant enzyme L104P
0.003
5-enolpyruvylshikimate 3-phosphate
-
recombinant overproducing strain
0.0052
5-enolpyruvylshikimate 3-phosphate
-
-
0.007
5-enolpyruvylshikimate 3-phosphate
-
H385N mutant enzyme
0.011
5-enolpyruvylshikimate 3-phosphate
-
wild-type enzyme
1.9
5-enolpyruvylshikimate 3-phosphate
-
+/-0.3 mM, mutant G99A
2.2
5-enolpyruvylshikimate 3-phosphate
-
+/-0.5 mM
2.5
5-enolpyruvylshikimate 3-phosphate
-
+/-0.4 mM, mutant L104P
0.0018
phosphate
-
-
0.0056
phosphate
-
25°C, pH 7.0, wild-type enzyme
0.0073
phosphate
-
25°C, pH 7.0, mutant enzyme G99A
0.0248
phosphate
-
25°C, pH 7.0, mutant enzyme L104P
0.343
phosphate
reverse reaction, in the presence of 50 mM KCl
1.2
phosphate
-
H385N mutant enzyme
4.6
phosphate
-
wild-type enzyme
7.3
phosphate
-
+/-0.7 mM, mutant G99A
24.8
phosphate
-
+/-4.8 mM, mutant L104P
0.0018
phosphoenolpyruvate
-
pH 7.0, 30°C, mutant StaroA-T42M
0.0024
phosphoenolpyruvate
-
pH 7.0, 30°C, mutant EcaroA-T42M
0.0027
phosphoenolpyruvate
-
-
0.0038
phosphoenolpyruvate
-
glyphosate-sensitive strain
0.0052
phosphoenolpyruvate
-
-
0.0052
phosphoenolpyruvate
-
cosubstrate 5-enolpyruvylshikimate 3-phosphate
0.007
phosphoenolpyruvate
-
glyphosate-resistant strain
0.008
phosphoenolpyruvate
-
-
0.009
phosphoenolpyruvate
-
glyphosate-sensitive enzyme
0.011
phosphoenolpyruvate
-
wild type enzyme, at 28°C, pH not specified in the publication
0.0113
phosphoenolpyruvate
-
expressed in Escherichia coli
0.015
phosphoenolpyruvate
-
wild-type enzyme
0.016
phosphoenolpyruvate
-
recombinant overproducing strain
0.017
phosphoenolpyruvate
-
in the presence of glyphosate
0.0181
phosphoenolpyruvate
plant
-
-
0.0199
phosphoenolpyruvate
wild-type enzyme
0.021 - 0.023
phosphoenolpyruvate
-
-
0.02167
phosphoenolpyruvate
-
at pH 7.0 and 30°C
0.022
phosphoenolpyruvate
in the presence of 100 mM NH4Cl
0.0223
phosphoenolpyruvate
-
pH 7.0, 30°C, wild-type EcaroA
0.023
phosphoenolpyruvate
in the presence of 1 mM NH4Cl and 100 mM KCl
0.025
phosphoenolpyruvate
-
-
0.025
phosphoenolpyruvate
mutant N130W
0.028
phosphoenolpyruvate
-
isoenzyme II
0.028
phosphoenolpyruvate
-
at pH 8.0 and 30°C
0.03
phosphoenolpyruvate
at pH 8.0 and 40°C
0.032
phosphoenolpyruvate
wild-type
0.032
phosphoenolpyruvate
-
mutant enzyme A187T, at 28°C, pH not specified in the publication
0.033
phosphoenolpyruvate
-
isoenzyme I
0.034
phosphoenolpyruvate
-
-
0.035
phosphoenolpyruvate
mutant N130S
0.04
phosphoenolpyruvate
C-terminal His6-tagged AroA, 50 mM KCl
0.0436
phosphoenolpyruvate
-
pH 7.0, 30°C, wild-type StaroA
0.045
phosphoenolpyruvate
wild-type
0.049
phosphoenolpyruvate
-
mutant enzyme T101A/A187T, at 28°C, pH not specified in the publication
0.05
phosphoenolpyruvate
50 mM KCl, mutant E341A
0.055
phosphoenolpyruvate
-
+/-0.0014 mM, mutant N130S in bacterial extact
0.055
phosphoenolpyruvate
-
+/-0.002 mM, bacterial extract
0.06
phosphoenolpyruvate
+/-0.006 mM
0.07
phosphoenolpyruvate
+/-0.005 mM, mutant P101S
0.071
phosphoenolpyruvate
mutant P101S
0.077
phosphoenolpyruvate
-
at pH 7.0 and 30°C
0.078
phosphoenolpyruvate
wild-type, pH 7.0, 28°C
0.078
phosphoenolpyruvate
-
wild type enzyme, at pH 7.0 and 28°C
0.08
phosphoenolpyruvate
+/-0.005 mM, mutant P101A
0.081
phosphoenolpyruvate
-
mutant enzyme T101A, at 28°C, pH not specified in the publication
0.085
phosphoenolpyruvate
in the presence of 1 mM NH4Cl and 10 mM KCl
0.088
phosphoenolpyruvate
-
wild-type enzyme
0.0883
phosphoenolpyruvate
mutant enzyme P106L
0.09
phosphoenolpyruvate
-
wild-type enzyme
0.09
phosphoenolpyruvate
+/-0.010 mM, mutant P101G
0.091
phosphoenolpyruvate
in the presence of 10 mM NH4Cl
0.0952
phosphoenolpyruvate
+/-0.00573 mM, full-length G2 EPSPS, crude lysate from overexpressing Escherichia coli ER2799, malachite green dye assay
0.0968
phosphoenolpyruvate
+/-0.01101 mM, reconstituted G2-EPSPS (by intein-mediated protein complementation), crude lysate from transformed Escherichia coli ER2799, malachite green dye assay
0.1
phosphoenolpyruvate
-
wild-type enzyme
0.1
phosphoenolpyruvate
in the presence of 1 mM NH4Cl
0.1
phosphoenolpyruvate
in presence of 1 mM shikimate 3-phosphate
0.1
phosphoenolpyruvate
mutant P101S/T97I
0.106
phosphoenolpyruvate
-
H385N mutant enzyme
0.14
phosphoenolpyruvate
-
glyphosate-resistant enzyme
0.148
phosphoenolpyruvate
mutant G96A, pH 7.0, 28°C
0.15
phosphoenolpyruvate
+/-0.020 mM, mutant P101L
0.15
phosphoenolpyruvate
C-terminal His6-tagged AroA, 100 mM KCl
0.16
phosphoenolpyruvate
wild-type AroA, 100 mM KCl
0.17
phosphoenolpyruvate
50 mM KCl, mutant E341C
0.18
phosphoenolpyruvate
-
pH 7.0, recombinant enzyme, in presence of 100 mM KCl
0.19
phosphoenolpyruvate
50 mM KCl, mutant D313N
0.195
phosphoenolpyruvate
pH 7.0, 28°C
0.2
phosphoenolpyruvate
-
R24D mutant enzyme
0.227
phosphoenolpyruvate
-
mutant enzyme G100A, at pH 7.0 and 28°C
0.23
phosphoenolpyruvate
wild-type, pH 7.0, temperature not specified in the publication
0.25
phosphoenolpyruvate
mutant E145G/N163H/N267S/P318R/M377V/M425T/P438L, pH 7.0, temperature not specified in the publication
0.26
phosphoenolpyruvate
-
at pH 8.0 and 37°C
0.28
phosphoenolpyruvate
50 mM KCl, mutant D313A
0.29
phosphoenolpyruvate
pH 7.0, 28°C
0.292
phosphoenolpyruvate
mutant T355S, pH 7.0, 28°C
0.31
phosphoenolpyruvate
mutant P318R, pH 7.0, temperature not specified in the publication
0.316
phosphoenolpyruvate
wild-type, pH 7.0, 28°C
0.32
phosphoenolpyruvate
mutant M425T, pH 7.0, temperature not specified in the publication
0.37
phosphoenolpyruvate
-
at pH 8.0 and 22°C
0.38
phosphoenolpyruvate
mutant T97I
0.38
phosphoenolpyruvate
mutant N267S, pH 7.0, temperature not specified in the publication
0.525
phosphoenolpyruvate
mutant N267S/P318R/M425T, pH 7.0, temperature not specified in the publication
0.59
phosphoenolpyruvate
-
P105S mutant enzyme
0.8
phosphoenolpyruvate
mutant G96A/A183T, in presence of 1 mM shikimate 3-phosphate
0.9
phosphoenolpyruvate
50 mM KCl, mutant E341Q
1
phosphoenolpyruvate
-
H385L mutant enzyme
1.3
phosphoenolpyruvate
50 mM KCl, mutant D313C
1.5
phosphoenolpyruvate
50 mM KCl, mutant H385A
1.6
phosphoenolpyruvate
50 mM KCl, mutant D313L
1.9
phosphoenolpyruvate
50 mM KCl, mutant E341M
2.5
phosphoenolpyruvate
50 mM KCl, mutant K22R value above 0.3
2.8
phosphoenolpyruvate
-
G96A mutant enzyme
3.1
phosphoenolpyruvate
mutant G96A, in presence of 1 mM shikimate 3-phosphate
3.8
phosphoenolpyruvate
mutant A183T, in presence of 1 mM shikimate 3-phosphate
4.762
phosphoenolpyruvate
-
mutant enzyme L105P, at pH 7.0 and 28°C
22.5
phosphoenolpyruvate
-
recombinant enzyme, in crude extracts
43.6
phosphoenolpyruvate
-
recombinant enzyme, in crude extracts
0.02
shikimate 3-phosphate
-
-
0.029
shikimate 3-phosphate
-
isoenzyme II
0.031
shikimate 3-phosphate
in the presence of 100 mM NH4Cl
0.033
shikimate 3-phosphate
-
isoenzyme I
0.049
shikimate 3-phosphate
in the presence of 1 mM NH4Cl and 10 mM KCl
0.06
shikimate 3-phosphate
+/-0.004 mM, mutant P101A
0.06
shikimate 3-phosphate
+/-0.006 mM
0.06
shikimate 3-phosphate
+/-0.010 mM, mutant P101G
0.07
shikimate 3-phosphate
-
H385 mutant enzyme
0.08
shikimate 3-phosphate
+/-0.006 mM, mutant P101S
0.1
shikimate 3-phosphate
+/-0.007 mM, mutant P101L
0.109
shikimate 3-phosphate
in the presence of 1 mM NH4Cl and 100 mM KCl
0.118
shikimate 3-phosphate
in the presence of 10 mM NH4Cl
0.12
shikimate 3-phosphate
-
wild-type and G96A mutant enzyme
0.135
shikimate 3-phosphate
-
wild-type enzyme
0.145
shikimate 3-phosphate
in the presence of 1 mM NH4Cl
0.16
shikimate 3-phosphate
-
H385 mutant enzyme
0.2
shikimate 3-phosphate
-
R24D mutant enzyme
0.33
shikimate 3-phosphate
-
wild-type enzyme
0.43
shikimate 3-phosphate
-
P105S mutant enzyme
additional information
additional information
-
-
-
additional information
additional information
-
kinetic study
-
additional information
additional information
-
kinetic study
-
additional information
additional information
-
kinetic parameters of native and diethyldicarbonate-inactivated enzyme
-
additional information
additional information
-
comparison of kinetic parameters from a variety of organism
-
additional information
additional information
-
kinetics, wild-type and mutant enzymes
-
additional information
additional information
-
kinetics, wild-type and mutant enzymes
-
additional information
additional information
stopped-flow kinetics
-
additional information
additional information
-
stopped-flow kinetics
-
additional information
additional information
small residue substitutions for Pro101 do not alter the KM for phosphoenolpyruvate and shikimate 3-phosphate
-
additional information
additional information
-
small residue substitutions for Pro101 do not alter the KM for phosphoenolpyruvate and shikimate 3-phosphate
-
additional information
phosphoenolpyruvate
KM/KI: 13, mutant P101S
additional information
phosphoenolpyruvate
-
KM/KI: 13, mutant P101S
additional information
phosphoenolpyruvate
KM/KI: 150
additional information
phosphoenolpyruvate
-
KM/KI: 150
additional information
phosphoenolpyruvate
KM/KI: 2.3, mutant P101L
additional information
phosphoenolpyruvate
-
KM/KI: 2.3, mutant P101L
additional information
phosphoenolpyruvate
KM/KI: 4.2, mutant P101A
additional information
phosphoenolpyruvate
-
KM/KI: 4.2, mutant P101A
additional information
phosphoenolpyruvate
KM/KI: 7.5, mutant P101G
additional information
phosphoenolpyruvate
-
KM/KI: 7.5, mutant P101G