Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
G99A | KM-value for 5-enolpyruvylshikimate 3-phosphate is 86% of the wild-type value, KM-value for phosphate is 1.3fold higher than wild-type value. Turnover-number for 5-enolpyruvylshikimate 3-phosphate is 6.2fold higher than wild-type value, turnover-number for phosphate is 5.4fold lower than wild-type value | Pseudomonas aeruginosa |
L104P | KM-value for 5-enolpyruvylshikimate 3-phosphate is 1.14fold higher than the wild-type value, KM-value for phosphate is 4.4fold higher than wild-type value. Turnover-number for 5-enolpyruvylshikimate 3-phosphate is 2.7fold lower than wild-type value, turnover-number for phosphate is 1.9fold lower than wild-type value | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | resistant to glyphosate | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0019 | - |
5-enolpyruvylshikimate 3-phosphate | 25°C, pH 7.0, mutant enzyme G99A | Pseudomonas aeruginosa | |
0.002 | - |
5-enolpyruvylshikimate 3-phosphate | 25°C, pH 7.0, wild-type enzyme | Pseudomonas aeruginosa | |
0.0025 | - |
5-enolpyruvylshikimate 3-phosphate | 25°C, pH 7.0, mutant enzyme L104P | Pseudomonas aeruginosa | |
0.0056 | - |
phosphate | 25°C, pH 7.0, wild-type enzyme | Pseudomonas aeruginosa | |
0.0073 | - |
phosphate | 25°C, pH 7.0, mutant enzyme G99A | Pseudomonas aeruginosa | |
0.0248 | - |
phosphate | 25°C, pH 7.0, mutant enzyme L104P | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, SDS-PAGE | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.5 | - |
recombinant | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + 3-phosphoshikimate | - |
Pseudomonas aeruginosa | phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, SDS-PAGE | Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
phosphate | 25°C, pH 7.0, mutant enzyme G99A | Pseudomonas aeruginosa | |
1.2 | - |
5-enolpyruvylshikimate 3-phosphate | 25°C, pH 7.0, mutant enzyme G99A | Pseudomonas aeruginosa | |
2.7 | - |
5-enolpyruvylshikimate 3-phosphate | 25°C, pH 7.0, mutant strain L104P | Pseudomonas aeruginosa | |
2.8 | - |
phosphate | 25°C, pH 7.0, mutant enzyme L104P | Pseudomonas aeruginosa | |
5.4 | - |
phosphate | 25°C, pH 7.0, wild-type enzyme | Pseudomonas aeruginosa | |
7.4 | - |
5-enolpyruvylshikimate 3-phosphate | 25°C, pH 7.0, wild-type enzyme | Pseudomonas aeruginosa |