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Literature summary for 2.5.1.19 extracted from

  • Tian, Y.S.; Xu, J.; Xiong, A.S.; Zhao, W.; Fu, X.Y.; Peng, R.H.; Yao, Q.H.
    Improvement of glyphosate resistance through concurrent mutations in three amino acids of the Ochrobactrum 5-enopyruvylshikimate-3-phosphate synthase (2011), Appl. Environ. Microbiol., 77, 8409-8414.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.19

Application

Application Comment Organism
agriculture Arabidopsis thaliana transgenic plants expressing mutant E145G/N163H/N267S/P318R/M377V/M425T/P438L grow well in presence of 0.75 mM glyphosate, whereas Arabidopsis thaliana plants expressing wild-type enzyme grow only in presence of 0.25 mM glyphosate Brucella anthropi

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Brucella anthropi

Crystallization (Commentary)

Crystallization (Comment) Organism
three-dimensional structure modeling of mutations P318R and M425T. Results indicate that the enzyme's lower hemisphere’s upper surface is possibly another important region for glyphosate resistance Brucella anthropi

Protein Variants

Protein Variants Comment Organism
E145G/N163H/N267S/P318R/M377V/M425T/P438L mutant is able to restore the growth of the Escherichia coli mutant ER2799, with the 5-enolpyruvylshikimate-3-phosphate synthase gene deleted from its genome, on an M9 minimal medium containing 300 mM glyphosate. The effectiveness of amino acid alterations N267S, P318R, and M245T on glyphosate tolerance is in the decreasing order P318R, M425T, N267S Brucella anthropi
M425T mutation identified in a multiple mutant enzyme resistant to glyphosate Brucella anthropi
N267S mutation identified in a multiple mutant enzyme resistant to glyphosate Brucella anthropi
N267S/P318R/M425T mutant enzyme shows high tolerance against glyphosate Brucella anthropi
P318R mutation identified in a multiple mutant enzyme resistant to glyphosate Brucella anthropi

Inhibitors

Inhibitors Comment Organism Structure
glyphosate
-
 Brucella anthropi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.23
-
 phosphoenolpyruvate wild-type, pH 7.0, temperature not specified in the publication Brucella anthropi
0.25
-
 phosphoenolpyruvate mutant E145G/N163H/N267S/P318R/M377V/M425T/P438L, pH 7.0, temperature not specified in the publication Brucella anthropi
0.31
-
 phosphoenolpyruvate mutant P318R, pH 7.0, temperature not specified in the publication Brucella anthropi
0.32
-
 phosphoenolpyruvate mutant M425T, pH 7.0, temperature not specified in the publication Brucella anthropi
0.38
-
 phosphoenolpyruvate mutant N267S, pH 7.0, temperature not specified in the publication Brucella anthropi
0.525
-
 phosphoenolpyruvate mutant N267S/P318R/M425T, pH 7.0, temperature not specified in the publication Brucella anthropi

Organism

Organism UniProt Comment Textmining
Brucella anthropi A6WUV8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + 3-phosphoshikimate
-
 Brucella anthropi phosphate + 5-enolpyruvylshikimate 3-phosphate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
26.7
-
 phosphoenolpyruvate wild-type, pH 7.0, temperature not specified in the publication Brucella anthropi
31.7
-
 phosphoenolpyruvate mutant N267S, pH 7.0, temperature not specified in the publication Brucella anthropi
35
-
 phosphoenolpyruvate mutant M425T, pH 7.0, temperature not specified in the publication Brucella anthropi
43.3
-
 phosphoenolpyruvate mutant P318R, pH 7.0, temperature not specified in the publication Brucella anthropi
51.7
-
 phosphoenolpyruvate mutant E145G/N163H/N267S/P318R/M377V/M425T/P438L, pH 7.0, temperature not specified in the publication Brucella anthropi
51.7
-
 phosphoenolpyruvate mutant N267S/P318R/M425T, pH 7.0, temperature not specified in the publication Brucella anthropi

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.069
-
 glyphosate wild-type, pH 7.0, temperature not specified in the publication Brucella anthropi
0.08
-
 glyphosate mutant N267S, pH 7.0, temperature not specified in the publication Brucella anthropi
0.091
-
 glyphosate mutant M425T, pH 7.0, temperature not specified in the publication Brucella anthropi
0.11
-
 glyphosate mutant P318R, pH 7.0, temperature not specified in the publication Brucella anthropi
0.15
-
 glyphosate mutant N267S/P318R/M425T, pH 7.0, temperature not specified in the publication Brucella anthropi
0.16
-
 glyphosate mutant E145G/N163H/N267S/P318R/M377V/M425T/P438L, pH 7.0, temperature not specified in the publication Brucella anthropi

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.61
-
 wild-type, pH 7.0, temperature not specified in the publication Brucella anthropi glyphosate
0.69
-
 mutant N267S, pH 7.0, temperature not specified in the publication Brucella anthropi glyphosate
0.73
-
 mutant M425T, pH 7.0, temperature not specified in the publication Brucella anthropi glyphosate
0.81
-
 mutant P318R, pH 7.0, temperature not specified in the publication Brucella anthropi glyphosate
0.91
-
 mutant N267S/P318R/M425T, pH 7.0, temperature not specified in the publication Brucella anthropi glyphosate
0.92
-
 mutant E145G/N163H/N267S/P318R/M377V/M425T/P438L, pH 7.0, temperature not specified in the publication Brucella anthropi glyphosate

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
83.3
-
 phosphoenolpyruvate mutant N267S, pH 7.0, temperature not specified in the publication Brucella anthropi
109.3
-
 phosphoenolpyruvate mutant M425T, pH 7.0, temperature not specified in the publication Brucella anthropi
115.9
-
 phosphoenolpyruvate wild-type, pH 7.0, temperature not specified in the publication Brucella anthropi
139.8
-
 phosphoenolpyruvate mutant P318R, pH 7.0, temperature not specified in the publication Brucella anthropi
206.7
-
 phosphoenolpyruvate mutant E145G/N163H/N267S/P318R/M377V/M425T/P438L, pH 7.0, temperature not specified in the publication Brucella anthropi
206.7
-
 phosphoenolpyruvate mutant N267S/P318R/M425T, pH 7.0, temperature not specified in the publication Brucella anthropi