Application | Comment | Organism |
---|---|---|
biotechnology | conferring glyphosate tolerance in transgenic crop plants | Bacteria |
Cloned (Comment) | Organism |
---|---|
from genomic DNA, in cosmid vector for phage packaging and transfection in Escherichia coli JM101 or in vitro transposon mutagenesis and transformation in Escherichia coli XL1 Blue MRF, in pAX306 for transformation of Escherichia coli DH5alpha and XL1 Blue MRF, in pRSF1b for inducible expression in Escherichia coli, in pSB11 shuttle vector for integration into Agrobacterium tumefaciens LBA4404 (already harbouring pSB1) and subsequent transformation of positive Agrobacterium strains in maize embryo for generation of transgenic plants | Bacteria |
Protein Variants | Comment | Organism |
---|---|---|
DELTAN1-22 | lacking N-terminal 22 residues including critical amino acid Lys20, fails to provide glyphosate tolerance compared to full-length enzyme when transformed in glyphosate-sensitive Escherichia coli DH5alpha and XL1 Blue | Bacteria |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glyphosate | competitor with phosphoenolpyruvate binding site, AroA(1398) transformation allows Escherichia coli DH5alpha and XL1 Blue colonies to grow on up to 200 mM glyphosate (non-transformed bacteria stop growing on 5 mM inhibitor), AroA(1398) integrated maize transgenic plant lines showed 54% survival upon glyphosate treatment compared to none of control plants | Bacteria | |
glyphosate | plants do not survive glyphosate spray treatment (1.78 l/ha of a 480 g AE/L formulation) in the field | plant |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0113 | - |
phosphoenolpyruvate | expressed in Escherichia coli | Bacteria | |
0.0181 | - |
phosphoenolpyruvate | - |
plant |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacteria | - |
selected from soil by its ability to grow in presence of the herbicide glyphosate | - |
Bacteria ATX1398 | - |
selected from soil by its ability to grow in presence of the herbicide glyphosate | - |
plant | - |
maize | - |
Purification (Comment) | Organism |
---|---|
from bacterial lysate by single step cobalt affinity chromatography followed by dialysis | Bacteria |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
35.9 | - |
Vmax | Bacteria |
47.5 | - |
Vmax | plant |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
shikimate 3-phosphate + phosphoenolpyruvate | pH 7, start with phosphoenolpyruvate, with or without inhibitor glyphosate | Bacteria | phosphate + 5-enolpyruvylshikimate 3-phosphate | fluorescence-based coupled enzymatic assay including xanthine oxidase, purine nucleoside phosphorylase, inosine, horseradish peroxidase and Amplex Red | ? | |
shikimate 3-phosphate + phosphoenolpyruvate | pH 7, start with phosphoenolpyruvate, with or without inhibitor glyphosate | plant | phosphate + 5-enolpyruvylshikimate 3-phosphate | fluorescence-based coupled enzymatic assay including xanthine oxidase, purine nucleoside phosphorylase, inosine, horseradish peroxidase and Amplex Red | ? | |
shikimate 3-phosphate + phosphoenolpyruvate | pH 7, start with phosphoenolpyruvate, with or without inhibitor glyphosate | Bacteria ATX1398 | phosphate + 5-enolpyruvylshikimate 3-phosphate | fluorescence-based coupled enzymatic assay including xanthine oxidase, purine nucleoside phosphorylase, inosine, horseradish peroxidase and Amplex Red | ? |
Synonyms | Comment | Organism |
---|---|---|
5-enolpyruvylshikimate-3phosphate synthase | - |
Bacteria |
5-enolpyruvylshikimate-3phosphate synthase | - |
plant |
AroA(1398) | glyphosate-tolerant gene product from strain ATX1398 that lacks protein sequence hallmarks of class II EPSP synthases | Bacteria |
AroA1398 | - |
Bacteria |
enolpyruvylshikimate phosphate synthase | - |
Bacteria |
EPSP synthase | - |
Bacteria |
maize EPSP synthase | - |
plant |
maize EPSPS | - |
plant |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
28.3 | - |
phosphoenolpyruvate | - |
Bacteria | |
38.4 | - |
phosphoenolpyruvate | - |
plant |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
glyphosate | KI/KM (phosphoenolpyruvate): 0.01 | plant | |
additional information | - |
glyphosate | KI/KM (phosphoenolpyruvate): 14 | Bacteria | |
0.0002 | - |
glyphosate | - |
plant | |
0.161 | - |
glyphosate | - |
Bacteria |