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2.5.1.19: 3-phosphoshikimate 1-carboxyvinyltransferase

This is an abbreviated version!
For detailed information about 3-phosphoshikimate 1-carboxyvinyltransferase, go to the full flat file.

Word Map on EC 2.5.1.19

Reaction

phosphoenolpyruvate
+
3-phosphoshikimate
=
phosphate
 +
5-O-(1-carboxyvinyl)-3-phosphoshikimate

Synonyms

3-enolpyruvylshikimate 5-phosphate synthase, 3-enolpyruvylshikimic acid-5-phosphate synthetase, 3-phosphoshikimate 1-carboxyvinyl-transferase, 3-phosphoshikimate 1-carboxyvinyltransferase, 3-phosphoshikimate-1-carboxyvinyl-transferase, 5'-enolpyruvylshikimate-3-phosphate synthase, 5-enol pyruvylshikimate 3-phosphate synthase, 5-enol-pyruvyl-shikimate-3-phosphate synthase, 5-enol-pyruvylshikimate 3-phosphate synthase, 5-enol-pyruvylshikimate-3-phosphate synthase, 5-enolpyruvyl-3-phosphoshikimate synthase, 5-enolpyruvylshikimate 3-phosphate synthase, 5-enolpyruvylshikimate-3-phosphate synthase, 5-enolpyruvylshikimate-3-phosphate synthetase, 5-enolpyruvylshikimate-3-phosphoric acid synthase, 5-enolpyruvylshikimate-3phosphate synthase, 5-enolpyruvylshikimic acid-3-phosphate synthase, 5-enopyruvylshikimate-3-phosphate synthase, 5-enoylpyruvylshikimate 3-phosphate synthase, 5-enoylpyruvylshikimate-3-phosphate synthase, A1501 EPSPS, AM79, AroA, AroA(1398), AroA(A1501), AroA1398, CaEPSPS, cp4 epsps, DsaroA, Dunaliella salina 5-enolpyruvylshikimate-3-phosphate synthase, Dunaliella salina EPSP synthase, E. coli EPSPS, EcaroA, enolpyruvylshikimate 3-phosphate synthase, enolpyruvylshikimate phosphate synthase, enolpyruvylshikimate-3-phosphate synthase, EPSP synhthase, EPSP synthase, EPSPS, G2 5-enolpyruvyl shikimate 3-phosphate synthase, G2 EPSPS, GR79Ms, maize EPSP synthase, maize EPSPS, mEPSPS, More, Mt EPSPS, Mycobacterium tuberculosis 5-enolpyruvylshikimate-3-phosphate synthase, Potri.002G146400, StaroA, synthase, 5-enolpyruvoylshikimate 3-phosphate

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.19 3-phosphoshikimate 1-carboxyvinyltransferase

Purification

Purification on EC 2.5.1.19 - 3-phosphoshikimate 1-carboxyvinyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, anion exchange chromatography, gel filtration, chromatofocusing
-
ammonium sulfate, DEAE-cellulose, hydroxyapatite, phenylaggarose, Sephacryl S-200, isozymes I, II and III
-
ammonium sulfate, DEAE-Sephacel, cellulose phosphate column, isoenzymes I and II
-
ammonium sulfate, DEAE-Sephacel, Mono Q, cellulose phosphate
-
ammonium sulfate, DEAE-Sephacel, phenyl-Sepharose, cellulose phosphate
-
DEAE-cellulose, CM-cellulose, partial purification
-
enzyme is part of multifunctional protein with distinct domains
-
from bacterial lysate by single step cobalt affinity chromatography followed by dialysis
-
from crude cell extracts by DEAE-sepharose anion-exchange chromatography (Pharmacia XK-16/40 column, elution with linear NaCl gradient), Superdex 200 gel filtration chromatography (Pharmacia Superdex 200 HR 10/30 column coupled to FPLC, pH 6.8), Resource Q anion-exchange chromatography (elution with linear NaCl gradient, pH 6.8)
-
from supernatant from 25% (w/v) ammonium sulfate precipitation of crude bacterial extracts, Q-sepharose anion exchange chromatography (NaCl gradient elution) and FPLC (Mono-Q column, NaCl gradient elution)
glyphosate insensitve strain, ammonium sulfate, heat treatment, DEAE-cellulose, Sephadex G-75, cellulose phosphate, chromatofocusing
-
His-Bind agarose resin column chromatography and Superdex 200 gel filtration
HisTrap nickel affinity column chromatography and HiPrep 26/60 Superdex gel filtration
HiTrap column chromatography and Superdex 200 gel filtration
-
Ni-NTA column chromatography
Ni-NTA resin column chromatography
-
Ni2+-NTA agarose affinity column chromatography
-
Ni2+-NTA agarose column chromatography
-
Ni2+-NTA agarose column chromatography, and gel filtration
recombinant enzyme
recombinant enzyme from Escherichia coli strain BL21(DE3)
-
recombinant enzyme, ammonium sulfate
-
recombinant enzyme, ammonium sulfate, DEAE-Sephacel, phenyl-Sepharose, phosphocellulose chromatography
-
recombinant enzyme, ammonium sulfate, hydrophobic interaction chromatography, anion-exchange chromatography
recombinant enzyme, Q-Sepharose, one step purification
-
recombinant wild-type and G96A mutant enzyme
-
using an anion exchange Q-Sepharose column, ammonium sulfate precipitation using an equilibrated phenyl Sepharose column
-
using Ni-NTA chromatography