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Literature summary for 2.5.1.19 extracted from

  • Zhou, M.; Xu, H.; Wei, X.; Ye, Z.; Wei, L.; Gong, W.; Wang, Y.; Zhu, Z.
    Identification of a glyphosate-resistant mutant of rice 5-enolpyruvylshikimate 3-phosphate synthase using a directed evolution strategy (2006), Plant Physiol., 140, 184-195.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Oryza sativa

Protein Variants

Protein Variants Comment Organism
P106L P106L mutant has a high glyphosate resistance while retaining relatively high catalytic efficiency at low phosphoenolpyruvate concentrations. 3-fold increase in glyphosate resistance of Escherichia coli expressing the P106L mutant. Affinity of the P106L mutant for glyphosate and phosphoenolpyruvate is decreased about 70-fold and 4.6-fold, respectively, compared to wild-type Oryza sativa

Inhibitors

Inhibitors Comment Organism Structure
glyphosate
-
Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0199
-
phosphoenolpyruvate wild-type enzyme Oryza sativa
0.0883
-
phosphoenolpyruvate mutant enzyme P106L Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphoenolpyruvate + 3-phosphoshikimate Oryza sativa
-
phosphate + 5-enolpyruvylshikimate 3-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Oryza sativa Q93VK6
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.03
-
wild-type enzyme Oryza sativa
10.23
-
mutant enzyme P106L Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + 3-phosphoshikimate
-
Oryza sativa phosphate + 5-enolpyruvylshikimate 3-phosphate
-
?

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00898
-
wild-type enzyme Oryza sativa glyphosate
0.1046
-
mutant enzyme P106L Oryza sativa glyphosate