Cloned (Comment) | Organism |
---|---|
- |
Oryza sativa |
Protein Variants | Comment | Organism |
---|---|---|
P106L | P106L mutant has a high glyphosate resistance while retaining relatively high catalytic efficiency at low phosphoenolpyruvate concentrations. 3-fold increase in glyphosate resistance of Escherichia coli expressing the P106L mutant. Affinity of the P106L mutant for glyphosate and phosphoenolpyruvate is decreased about 70-fold and 4.6-fold, respectively, compared to wild-type | Oryza sativa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glyphosate | - |
Oryza sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0199 | - |
phosphoenolpyruvate | wild-type enzyme | Oryza sativa | |
0.0883 | - |
phosphoenolpyruvate | mutant enzyme P106L | Oryza sativa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + 3-phosphoshikimate | Oryza sativa | - |
phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | Q93VK6 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.03 | - |
wild-type enzyme | Oryza sativa |
10.23 | - |
mutant enzyme P106L | Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + 3-phosphoshikimate | - |
Oryza sativa | phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
? |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00898 | - |
wild-type enzyme | Oryza sativa | glyphosate | |
0.1046 | - |
mutant enzyme P106L | Oryza sativa | glyphosate |