Cloned (Comment) | Organism |
---|---|
expression of the chimeric mutant aroA-M1, comprising N-terminal part of the Escherichia coli enzyme and the C-terminal part of the enzyme from Salmonella typhimurium in Escherichia coli strain XL1-Blue | Salmonella enterica subsp. enterica serovar Typhimurium |
expression of the wild-type enzyme and of the chimeric mutant aroA-M1, comprising N-terminal part of the Escherichia coli enzyme and the C-terminal part of the enzyme from Salmonella typhimurium in strain XL1-Blue | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of chimeric mutants by fusing the N-terminal part of the Escherichia coli enzyme with the C-terminal part of the enzyme from Salmonella typhimurium, i.e. mutant construct aroA-M1, the chimeric mutant shows a 6fold lower Km for phosphoenolpyruvate, a 5fold higher Ki for glyphosate, and a 9fold higher specific activity than the wild-type enzyme from Escherichia coli, construction of glyphosate-resistant transgenic Nicotiana tabacum plants expressing the chimeric mutant aroA-M1 using the Agrobacterium tumefaciens strain LBA 4404 infection system | Salmonella enterica subsp. enterica serovar Typhimurium |
additional information | construction of chimeric mutants by fusing the N-terminal part of the Escherichia coli enzyme with the C-terminal part of the enzyme from Salmonella typhimurium, the chimeric mutant shows a 6fold lower Km for phosphoenolpyruvate, a 5fold higher Ki for glyphosate, and a 9fold higher specific activity than the wild-type enzyme from Escherichia coli, construction of glyphosate-resistant transgenic Nicotiana tabacum plants expressing the chimeric mutant aroA-M1 using the Agrobacterium tumefaciens strain LBA 4404 infection system | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + 3-phosphoshikimate | Salmonella enterica subsp. enterica serovar Typhimurium | key enzyme in the shikimate pathway | phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate | - |
? | |
phosphoenolpyruvate + 3-phosphoshikimate | Escherichia coli | key enzyme in the shikimate pathway | phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene aroA | - |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
gene aroA | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + 3-phosphoshikimate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate | - |
? | |
phosphoenolpyruvate + 3-phosphoshikimate | - |
Escherichia coli | phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate | - |
? | |
phosphoenolpyruvate + 3-phosphoshikimate | key enzyme in the shikimate pathway | Salmonella enterica subsp. enterica serovar Typhimurium | phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate | - |
? | |
phosphoenolpyruvate + 3-phosphoshikimate | key enzyme in the shikimate pathway | Escherichia coli | phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5-enolpyruvylshikimate-3-phosphate synthase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
5-enolpyruvylshikimate-3-phosphate synthase | - |
Escherichia coli |
EPSPS | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
EPSPS | - |
Escherichia coli |