Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.4.2.36: NAD+-diphthamide ADP-ribosyltransferase

This is an abbreviated version!
For detailed information about NAD+-diphthamide ADP-ribosyltransferase, go to the full flat file.

Word Map on EC 2.4.2.36

Reaction

NAD+
+
diphthamide-[translation elongation factor 2]
=
nicotinamide
 +
N-(ADP-D-ribosyl)diphthamide-[translation elongation factor 2]

Synonyms

(adenosine diphosphoribose)transferase, nicotinamide adenine dinucleotide-elongation factor 2, ADP-ribosyltransferase, cholera toxin, cholix, cholix toxin, chxA, CTB, diphthamide-specific ADPRT, diphthamide-specific mono-ADP-ribosylating toxin, EHI 155600, EhToxin-l, EhToxin-like, ExoA, ExoA(c), exotoxin A, mono(ADPribosyl)transferase, mono-ADP-ribosyltransferase, NAD(+)-diphthamide ADP-ribosyltransferase, NAD-diphthamide ADP-ribosyltransferase, NAD-diphthamide ADP-ribosyltransferase NAD-elongation factor 2 ADP-ribosyltransferase, NAD:elongation factor 2-adenosine diphosphate ribose-transferase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.2 Pentosyltransferases
                2.4.2.36 NAD+-diphthamide ADP-ribosyltransferase

Purification

Purification on EC 2.4.2.36 - NAD+-diphthamide ADP-ribosyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
0.2 microm crossflow microfiltration, concentrated and diafiltered against 10 mM phosphate buffer, pH 7.6, with 30 kDa crossflow ultrafiltration, toxin bound on a S 5/50 GL cation exchange column and eluted with a salt gradient of 10 mM potassium phosphate buffer, pH 7.0 with 1 M NaCl
-
adsorption on Ni-NTA resin column, washing and elution with buffer containing 500 mM imidazole, dialyzing in 2 steps
-
HisTrap column chromatography and Superdex S200 gel filtration
-
Ni-NTA agarose column chromatography
recombinant catalytic fragment of ExoA, ExoAc
-