Application | Comment | Organism |
---|---|---|
medicine | therapy for autoimmune diabetes with glutamic acid decarboxylase 65 as autoantigen combined with cholera toxin B subunit as adjuvans lowering the number of antigens needed for autoimmune suppression, oral application for 5 weeks reduces insulitis of pancreas in mouse model | Vibrio cholerae serotype O1 |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) with expression vector pET28a | Vibrio cholerae serotype O1 |
Protein Variants | Comment | Organism |
---|---|---|
additional information | recombinant gene of cholera toxin B subunit with triple glutamic acid decarboxylase 65 peptides 531-545 (3p531) with 6-bp oligonucleotide sequences as flexible hinges | Vibrio cholerae serotype O1 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21000 | - |
SDS-PAGE of purified recombinant fusion enzyme cholera toxin B subunit with glutamic acid carboxylase 65 peptides 531-545 with histidine 6 tag | Vibrio cholerae serotype O1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio cholerae serotype O1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
adsorption on Ni-NTA resin column, washing and elution with buffer containing 500 mM imidazole, dialyzing in 2 steps | Vibrio cholerae serotype O1 |
Synonyms | Comment | Organism |
---|---|---|
CTB | cholera toxin B subunit | Vibrio cholerae serotype O1 |