2.4.2.10: orotate phosphoribosyltransferase

This is an abbreviated version!
For detailed information about orotate phosphoribosyltransferase, go to the full flat file.

Reaction

Synonyms

More, OMP synthase, OPRT, OPRTase, orotate phosphoribosyl pyrophosphate transferase, orotate phosphoribosyl transferase, orotate phosphoribosyltransferase, orotate PRTase, orotic acid phosphoribosyltransferase, orotidine 5'-monophosphate pyrophosphorylase, orotidine 5'-phosphate pyrophosphorylase, orotidine monophosphate pyrophosphorylase, orotidine phosphoribosyltransferase, orotidine-5'-phosphate pyrophosphorylase, orotidylate phosphoribosyltransferase, orotidylate pyrophosphorylase, orotidylic acid phosphorylase, orotidylic acid pyrophosphorylase, orotidylic phosphorylase, orotidylic pyrophosphorylase, phosphoribosyltransferase, orotate, Pyr5, PyrE, rv0382c, type I phosphoribosyltransferase, ura5

ECTree

     2 Transferases

         2.4 Glycosyltransferases

             2.4.2 Pentosyltransferases

                2.4.2.10 orotate phosphoribosyltransferase

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Results	in table
1	Activating Compound
44599	AA Sequence
10	Application
1	CAS Registry Number
28	Cloned(Commentary)
9	Crystallization (Commentary)
1113	Disease/ Diagnostics
6	Expression
4	General Information
9	General Stability
136	Inhibitors
18	kcat/KM [mM/s]
93	Ki Value [mM]
93	KM Value [mM]
12	Localization
40	Metals/Ions
35	Molecular Weight [Da]
36	Natural Substrates/ Products (Substrates)
94	Organism
8	Pathway
108	PDB ID
15	pH Optimum
4	pH Range
2	pH Stability
2	pI Value
12	Protein Variants
31	Purification (Commentary)
8	Reaction
1	Reaction Type
91	Reference
52	Source Tissue
30	Specific Activity [micromol/min/mg]
11	Storage Stability
103	Substrates and Products (Substrate)
28	Subunits
98	Synonyms
1	Systematic Name
12	Temperature Optimum [°C]
3	Temperature Stability [°C]
29	Turnover Number [1/s]

Storage Stability

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of the Enzyme Summary Page.

Storage Stability on EC 2.4.2.10 - orotate phosphoribosyltransferase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-20°C, concentrated enzyme solution, at least 4 months

Mus musculus

-

489713

-20°C, pH 7.5-9.5, at least 6 months

Saccharomyces cerevisiae

-

489715

-25°C, in 50% v/v glycerol, 0.1 mM DTT, about 35% loss of activity within 40 days

Escherichia coli

-

489720

-60°C to-20°C, crude enzyme or ammonium sulfate preparation, 2 years

Mus musculus

-

489712

-76°C, at least 6 months

Saccharomyces cerevisiae

-

489716

-80°C, stable up to 12 months, 5% glycerol, pH 8.0

Mycobacterium tuberculosis

P9WHK9

723161

4°C, 50 mM Tris-HCl, 2 mM EDTA, 2 mM mercaptoethanol, pH 6.4, several months, no apparent loss of activity

Corynebacterium ammoniagenes

-

687400

4°C, gradual loss of activity at pH 7.5-9.5

Saccharomyces cerevisiae

-

489715

4°C, purified recombinant bifunctional enzyme, stability property of both domains in the bifunctional enzyme in the absence or presence of 0.5 mM PRPP and 0.5 mM UMP: half-life of the OPRT activity is about 3-4 days and 3 weeks, respectively. The OMPDC activity is much more stable with half-life of about 3-4 weeks. 90% of the bifunctional protein, stored for 4 weeks, remains as a bifunctional protein band in SDS-PAGE and image analyses

Plasmodium falciparum

Q8N0R1

748831

4°C, t1/2: 1.5 days

Plasmodium falciparum

-

489721

frozen, partially purified enzyme in phosphate buffer, pH 7 or 7.5, 2 months

Mus musculus

-

489712