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Literature summary for 2.4.2.10 extracted from
- Isolation and initial characterization of the single polypeptide that synthesizes uridine 5-monophosphate from orotate in Ehrlich ascites carcinoma. Purification by tandem affinity chromatography of uridine-5-monophosphate synthase (1980), Biochemistry, 19, 4699-4706.
General Stability
| General Stability | Organism |
|---|---|
| 0.05 mM UMP and 1% polyethyleneglycol stabilize during final stage of purification | Mus musculus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
sucrose density gradient centrifugation | Mus musculus |
| 51500 | - |
1 * 51500, SDS-PAGE, enzyme activity and orotidine 5-monophosphate decarboxylating activity on a single polypeptide | Mus musculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, rapid tandem affinity column method | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| Ehrlich ascites carcinoma cell | - |
Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.69 | - |
- |
Mus musculus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, concentrated enzyme solution, at least 4 months | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | enzyme from mammals is a bifunctional polypeptide, it also catalyzes the reaction listed as EC 4.1.1.23 | Mus musculus | ? | - |
? |  |
| orotate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Mus musculus | orotidine 5'-phosphate + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 51500, SDS-PAGE, enzyme activity and orotidine 5'-monophosphate decarboxylating activity on a single polypeptide | Mus musculus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | enzyme from mammals is a bifunctional polypeptide, it also catalyzes the reaction listed as EC 4.1.1.23 | Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
2 isozymes: pI: 5.65 (minor form), pI: 5.85 (major form) | Mus musculus |
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Release 2023.1 (January 2023)
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