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Literature summary for 2.4.2.10 extracted from

  • Breda, A.; Rosado, L.; Lorenzini, D.; Basso, L.; Santos, D.
    Molecular, kinetic and thermodynamic characterization of Mycobacterium tuberculosis orotate phosphoribosyltransferase (2012), Mol. Biosyst., 8, 572-586.
    View publication on PubMed
Search for more literature for 2.4.2.10

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
diphosphate product inhibition Mycobacterium tuberculosis
orotidine 5'-phosphate product inhibition Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0026
-
 orotidine 5'-phosphate ping-pong mechanism, pH 8.0, 25°C Mycobacterium tuberculosis
0.009
-
 5-phospho-alpha-D-ribose 1-diphosphate ordered mechanism, pH 8.0, 25°C Mycobacterium tuberculosis
0.0094
-
 Orotate ordered mechanism, pH 8.0, 25°C Mycobacterium tuberculosis
0.01
-
 Orotate ping-pong mechanism, pH 8.0, 25°C Mycobacterium tuberculosis
0.016
-
 diphosphate ping-pong mechanism, pH 8.0, 25°C Mycobacterium tuberculosis
0.096
-
 5-phospho-alpha-D-ribose 1-diphosphate ping-pong mechanism, pH 8.0, 25°C Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18761
-
 2 * 20000, SDS-PAGE, gel filtration; 2 * 18761, mass spectrometry, gel filtration Mycobacterium tuberculosis
20000
-
 2 * 20000, SDS-PAGE, gel filtration; 2 * 18761, mass spectrometry, gel filtration Mycobacterium tuberculosis
38000
-
 gel filtration Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WHK9
-
-
Mycobacterium tuberculosis H37Rv P9WHK9
-
-

Purification (Commentary)

Purification (Comment) Organism
anion exchange chromatography, gel filtration, hydrophobic interaction chromatography Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
105
-
 pH 8.0, 25°C Mycobacterium tuberculosis

Storage Stability

Storage Stability Organism
-80°C, stable up to 12 months, 5% glycerol, pH 8.0 Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
orotate + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Mycobacterium tuberculosis orotidine 5'-phosphate + diphosphate
-
 r
orotate + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Mycobacterium tuberculosis H37Rv orotidine 5'-phosphate + diphosphate
-
 r
orotidine 5'-phosphate + diphosphate
-
 Mycobacterium tuberculosis orotate + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
orotidine 5'-phosphate + diphosphate
-
 Mycobacterium tuberculosis H37Rv orotate + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r

Subunits

Subunits Comment Organism
homodimer 2 * 20000, SDS-PAGE, gel filtration; 2 * 18761, mass spectrometry, gel filtration Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
orotate phosphoribosyltransferase
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.28
-
 diphosphate ping-pong mechanism, pH 8.0, 25°C Mycobacterium tuberculosis
0.28
-
 orotidine 5'-phosphate ping-pong mechanism, pH 8.0, 25°C Mycobacterium tuberculosis
0.6
-
 Orotate ordered mechanism, pH 8.0, 25°C Mycobacterium tuberculosis
0.61
-
 Orotate ping-pong mechanism, pH 8.0, 25°C Mycobacterium tuberculosis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00044
-
 orotidine 5'-phosphate mixed type inhibition, 5-phospho-alpha-D-ribose 1-diphosphate varied substrate, orotate unsaturated, pH 8.0, 25°C Mycobacterium tuberculosis
0.001
-
 orotidine 5'-phosphate mixed type inhibition, 5-phospho-alpha-D-ribose 1-diphosphate varied substrate, orotate saturated, pH 8.0, 25°C Mycobacterium tuberculosis
0.0074
-
 orotidine 5'-phosphate mixed type inhibition, orotate varied substrate, 5-phospho-alpha-D-ribose 1-diphosphate unsaturated, pH 8.0, 25°C Mycobacterium tuberculosis
0.026
-
 diphosphate mixed type inhibition, 5-phospho-alpha-D-ribose 1-diphosphate varied substrate, orotate unsaturated, pH 8.0, 25°C Mycobacterium tuberculosis
0.029
-
 diphosphate mixed type inhibition, orotate varied substrate, 5-phospho-alpha-D-ribose 1-diphosphate unsaturated, pH 8.0, 25°C Mycobacterium tuberculosis
0.035
-
 orotidine 5'-phosphate noncompetitive, orotate varied substrate, 5-phospho-alpha-D-ribose 1-diphosphate saturated, pH 8.0, 25°C Mycobacterium tuberculosis
0.108
-
 diphosphate noncompetitive, 5-phospho-alpha-D-ribose 1-diphosphate varied substrate, orotate saturated, pH 8.0, 25°C Mycobacterium tuberculosis
0.209
-
 diphosphate mixed type inhibition, orotate varied substrate, 5-phospho-alpha-D-ribose 1-diphosphate saturated, pH 8.0, 25°C Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6
-
 5-phospho-alpha-D-ribose 1-diphosphate ping-pong mechanism, pH 8.0, 25°C Mycobacterium tuberculosis
6.6
-
 5-phospho-alpha-D-ribose 1-diphosphate ordered mechanism, pH 8.0, 25°C Mycobacterium tuberculosis
10
-
 diphosphate ping-pong mechanism, pH 8.0, 25°C Mycobacterium tuberculosis
60
-
 Orotate ping-pong mechanism, pH 8.0, 25°C Mycobacterium tuberculosis
64
-
 Orotate ordered mechanism, pH 8.0, 25°C Mycobacterium tuberculosis
100
-
 orotidine 5'-phosphate ping-pong mechanism, pH 8.0, 25°C Mycobacterium tuberculosis