Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphosphate | product inhibition | Mycobacterium tuberculosis | |
orotidine 5'-phosphate | product inhibition | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0026 | - |
orotidine 5'-phosphate | ping-pong mechanism, pH 8.0, 25°C | Mycobacterium tuberculosis | |
0.009 | - |
5-phospho-alpha-D-ribose 1-diphosphate | ordered mechanism, pH 8.0, 25°C | Mycobacterium tuberculosis | |
0.0094 | - |
Orotate | ordered mechanism, pH 8.0, 25°C | Mycobacterium tuberculosis | |
0.01 | - |
Orotate | ping-pong mechanism, pH 8.0, 25°C | Mycobacterium tuberculosis | |
0.016 | - |
diphosphate | ping-pong mechanism, pH 8.0, 25°C | Mycobacterium tuberculosis | |
0.096 | - |
5-phospho-alpha-D-ribose 1-diphosphate | ping-pong mechanism, pH 8.0, 25°C | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18761 | - |
2 * 20000, SDS-PAGE, gel filtration; 2 * 18761, mass spectrometry, gel filtration | Mycobacterium tuberculosis |
20000 | - |
2 * 20000, SDS-PAGE, gel filtration; 2 * 18761, mass spectrometry, gel filtration | Mycobacterium tuberculosis |
38000 | - |
gel filtration | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WHK9 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WHK9 | - |
- |
Purification (Comment) | Organism |
---|---|
anion exchange chromatography, gel filtration, hydrophobic interaction chromatography | Mycobacterium tuberculosis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
105 | - |
pH 8.0, 25°C | Mycobacterium tuberculosis |
Storage Stability | Organism |
---|---|
-80°C, stable up to 12 months, 5% glycerol, pH 8.0 | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
orotate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Mycobacterium tuberculosis | orotidine 5'-phosphate + diphosphate | - |
r | |
orotate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Mycobacterium tuberculosis H37Rv | orotidine 5'-phosphate + diphosphate | - |
r | |
orotidine 5'-phosphate + diphosphate | - |
Mycobacterium tuberculosis | orotate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
orotidine 5'-phosphate + diphosphate | - |
Mycobacterium tuberculosis H37Rv | orotate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 20000, SDS-PAGE, gel filtration; 2 * 18761, mass spectrometry, gel filtration | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
orotate phosphoribosyltransferase | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
diphosphate | ping-pong mechanism, pH 8.0, 25°C | Mycobacterium tuberculosis | |
0.28 | - |
orotidine 5'-phosphate | ping-pong mechanism, pH 8.0, 25°C | Mycobacterium tuberculosis | |
0.6 | - |
Orotate | ordered mechanism, pH 8.0, 25°C | Mycobacterium tuberculosis | |
0.61 | - |
Orotate | ping-pong mechanism, pH 8.0, 25°C | Mycobacterium tuberculosis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00044 | - |
orotidine 5'-phosphate | mixed type inhibition, 5-phospho-alpha-D-ribose 1-diphosphate varied substrate, orotate unsaturated, pH 8.0, 25°C | Mycobacterium tuberculosis | |
0.001 | - |
orotidine 5'-phosphate | mixed type inhibition, 5-phospho-alpha-D-ribose 1-diphosphate varied substrate, orotate saturated, pH 8.0, 25°C | Mycobacterium tuberculosis | |
0.0074 | - |
orotidine 5'-phosphate | mixed type inhibition, orotate varied substrate, 5-phospho-alpha-D-ribose 1-diphosphate unsaturated, pH 8.0, 25°C | Mycobacterium tuberculosis | |
0.026 | - |
diphosphate | mixed type inhibition, 5-phospho-alpha-D-ribose 1-diphosphate varied substrate, orotate unsaturated, pH 8.0, 25°C | Mycobacterium tuberculosis | |
0.029 | - |
diphosphate | mixed type inhibition, orotate varied substrate, 5-phospho-alpha-D-ribose 1-diphosphate unsaturated, pH 8.0, 25°C | Mycobacterium tuberculosis | |
0.035 | - |
orotidine 5'-phosphate | noncompetitive, orotate varied substrate, 5-phospho-alpha-D-ribose 1-diphosphate saturated, pH 8.0, 25°C | Mycobacterium tuberculosis | |
0.108 | - |
diphosphate | noncompetitive, 5-phospho-alpha-D-ribose 1-diphosphate varied substrate, orotate saturated, pH 8.0, 25°C | Mycobacterium tuberculosis | |
0.209 | - |
diphosphate | mixed type inhibition, orotate varied substrate, 5-phospho-alpha-D-ribose 1-diphosphate saturated, pH 8.0, 25°C | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6 | - |
5-phospho-alpha-D-ribose 1-diphosphate | ping-pong mechanism, pH 8.0, 25°C | Mycobacterium tuberculosis | |
6.6 | - |
5-phospho-alpha-D-ribose 1-diphosphate | ordered mechanism, pH 8.0, 25°C | Mycobacterium tuberculosis | |
10 | - |
diphosphate | ping-pong mechanism, pH 8.0, 25°C | Mycobacterium tuberculosis | |
60 | - |
Orotate | ping-pong mechanism, pH 8.0, 25°C | Mycobacterium tuberculosis | |
64 | - |
Orotate | ordered mechanism, pH 8.0, 25°C | Mycobacterium tuberculosis | |
100 | - |
orotidine 5'-phosphate | ping-pong mechanism, pH 8.0, 25°C | Mycobacterium tuberculosis |