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2.4.2.10: orotate phosphoribosyltransferase

This is an abbreviated version!
For detailed information about orotate phosphoribosyltransferase, go to the full flat file.

Word Map on EC 2.4.2.10

Reaction

orotidine 5'-phosphate
+
diphosphate
=
Orotate
+
5-phospho-alpha-D-ribose 1-diphosphate

Synonyms

More, OMP synthase, OPRT, OPRTase, orotate phosphoribosyl pyrophosphate transferase, orotate phosphoribosyl transferase, orotate phosphoribosyltransferase, orotate PRTase, orotic acid phosphoribosyltransferase, orotidine 5'-monophosphate pyrophosphorylase, orotidine 5'-phosphate pyrophosphorylase, orotidine monophosphate pyrophosphorylase, orotidine phosphoribosyltransferase, orotidine-5'-phosphate pyrophosphorylase, orotidylate phosphoribosyltransferase, orotidylate pyrophosphorylase, orotidylic acid phosphorylase, orotidylic acid pyrophosphorylase, orotidylic phosphorylase, orotidylic pyrophosphorylase, phosphoribosyltransferase, orotate, Pyr5, PyrE, rv0382c, type I phosphoribosyltransferase, ura5

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.2 Pentosyltransferases
                2.4.2.10 orotate phosphoribosyltransferase

Crystallization

Crystallization on EC 2.4.2.10 - orotate phosphoribosyltransferase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ligated with sulfate, homodimer
-
hanging drop vapor diffusion method. Enzyme in complex with phosphate: 0.1 M HEPES pH 7.5, 10% (w/v) PEG8000, 8% (v/v) ethylene glycol. Enzyme in complex with 5-phospho-alpha-D-ribose 1-diphosphate: 0.1 M HEPES pH 7.5, 10% (w/v) PEG6000, 5% (v/v) 2-methyl-2,4-pentanediol. Enzyme in complex with ferric dicitrate: 0.01 M iron(III) chloride hexahydrate, 0.1 M sodium citrate tribasic dehydrate pH 6.1, 10% (v/v) and Jeffamine M-600
hanging drop vapor diffusion method, using 100 mM ammonium sulfate, 30% (w/v) PEG 3350, at 4°C
sitting-drop vapour-diffusion method, polyethylene glycol 300
-
sitting drop vapour diffusion method, the apoenzyme is crystallized in 100 mM Tris-HCl, pH 7.4, 0.2 M magnesium acetate, and 26% (w/v) PEG 6000, crystals of OPRTase in complex with substrates (5.0 mM 5-phospho-alpha-D-ribose 1-diphosphate and 5.0 mM orotate) or product (5.0 mM orotidine 5'-phosphate) are grown in 100 mM sodium acetate, pH 4.6, 0.14 M ammonium acetate, and 38% (w/v) PEG 4000, crystals of the OMP complex are grown from solutions containing 100 mM Tris HCl, pH 7.4, 0.08 M magnesium acetate, and 28% (w/v) PEG 6000
complexed with orotidine monophosphate, homodimer, with catalytically important residues from one subunit available for the other
-
hanging-grop vapour-diffusion method, 2.3 M ammonium sulfate, pH 8.6