Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.3.2.5: glutaminyl-peptide cyclotransferase

This is an abbreviated version!
For detailed information about glutaminyl-peptide cyclotransferase, go to the full flat file.

Word Map on EC 2.3.2.5

Reaction

L-glutaminyl-peptide
=
5-Oxoprolyl-peptide
+
NH3

Synonyms

AtQC, cyclotransferase, glutaminyl-transfer ribonucleate, DromeQC, gamma-glutamylamine cyclotransferase, GGACT, glutamine cyclotransferase, glutaminyl cyclase, glutaminyl-peptide cyclotransferase-like protein, glutaminyl-tRNA cyclotransferase, golgi resident enzyme, Golgi resident glutaminyl cyclase, Golgi-resident enzyme, Golgi-resident glutaminyl cyclase, gQC, h-isoQC, h-QC, hQC, isoDromeQC, isoGlutaminyl cyclase, isoQC, PgQC, QC, QCT, Qpct, Qpct1, QPCTL, secretory glutaminyl cyclase, sQC, StQC

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.2 Aminoacyltransferases
                2.3.2.5 glutaminyl-peptide cyclotransferase

Purification

Purification on EC 2.3.2.5 - glutaminyl-peptide cyclotransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
chelating Sepharose column chromatography and butyl Sepharose column chromatography
enzyme is semi-purified from latex crude extracts by ion exchange chromatography on a CM Sephadex column C-50 and gel filtration
hanging-drop vapour-diffusion method, wild-type and mutant enzymes
-
His-tagged version
-
hydrophobic interaction chromatography, ion exchange chromatography, gel filtration
-
Ni-NTA column chromatography
Ni-NTA column chromatography and Sephacryl S-100 gel filtration
of 6His-tagged enzyme expressed in Pichia pastoris and enzyme expressed in Escherichia coli
-
partial
-
Q-Sepharose column chromatography
recombinant enzyme
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged wild-type enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography