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Literature summary for 2.3.2.5 extracted from
- Human pituitary glutaminyl cyclase: expression in insect cells and dye affinity purification (2003), Protein Expr. Purif., 32, 141-146.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Drosophila S2 cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| N49A | mutant enzyme is not glycosylated, kinetic properties are indistinguishable from unmutated enzyme | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.7 | - |
Gln-Gln | - |
Homo sapiens |  |
| 1.4 | - |
Gln-NH2 | - |
Homo sapiens | |
| 6.7 | - |
Gln-tert-butyl ester | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
x * 37000, SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Bos taurus |
| glycoprotein | N-glycan sugar moiety is bound to Asn49 in the recombinant enzyme. Presence or absence of glycan has no apparent effect on structure, stability, or enzymatic activity of the enzyme | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pituitary gland | - |
Homo sapiens | - |
| pituitary gland | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Gln-Gln | - |
Homo sapiens | pyroglutamyl-Gln + NH3 | - |
? | |
| Gln-tert-butyl ester | - |
Homo sapiens | pyroglutamyl-tert-butyl ester + NH3 | - |
? | |
| L-Gln-NH2 | - |
Homo sapiens | L-pyroglutamyl amide + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 37000, SDS-PAGE | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10 | - |
Gln-Gln | - |
Homo sapiens | |
| 13 | - |
Gln-NH2 | - |
Homo sapiens | |
| 16 | - |
Gln-tert-butyl ester | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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