2.1.1.260: rRNA small subunit pseudouridine methyltransferase Nep1
This is an abbreviated version!
For detailed information about rRNA small subunit pseudouridine methyltransferase Nep1, go to the full flat file.
Word Map on EC 2.1.1.260
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2.1.1.260
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rrnas
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jannaschii
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methanocaldococcus
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nucleolus
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hypermodified
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s-adenosylmethionine
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archaea
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three-hybrid
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rna-binding
- 2.1.1.260
- rrnas
- jannaschii
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methanocaldococcus
- nucleolus
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hypermodified
- s-adenosylmethionine
- archaea
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three-hybrid
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rna-binding
Reaction
Synonyms
Bowen-Conradi syndrome protein, Emg1, Emg1p, essential for mitotic growth 1, N1-specific pseudouridine methyltransferase, Nep1, Nep1 methyltransferase, Nep1p, nucleolar essential protein 1, pseudouridine N1-methyltransferase, pseudouridine-N1-specific methyltransferase
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General Information
General Information on EC 2.1.1.260 - rRNA small subunit pseudouridine methyltransferase Nep1
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evolution
malfunction
metabolism
physiological function
additional information
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Nep1 belongs to the SPOUT-class RNA methyltransferases, Nep1 subfamily
evolution
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Nep1 belongs to the SPOUT-class RNA methyltransferases, Nep1 subfamily
evolution
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Nep1 is a member of the SPOUT-family of methyltransferases
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a temperature-sensitive ScNEP1ts1 allele is isolated and reveals a strongly increased sensitivity to paromomycin, a translational inhibitor which binds to RNA, indicating that ribosome biogenesis within the nucleolus is probably affected. Candida albicans and human NEP1 heterologously complement the essential phenotype in a Saccharomyces cerevisiae nep1 deletion mutant, the ScNEP1 spindle/microtubule phenotype is not found with HsNEP1 and CaNEP1
malfunction
addition of SAM to the medium restores growth at elevated temperatures in yeast with temperature sensitive mutants of the yeast Nep1 protein
malfunction
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lethal phenotype of a DELTAnep1 deletion, deletions in ribosome quality and functional control genes lead to DELTAnep1 growth deficiency. Except for DELTArps18b, deletions in the identified ribosome biogenesis genes are synthetically lethal with DELTAnep1. The DELTAutp30 deletion itself has no phenotype but it enforces all nep1-1ts mutant phenotypes, utp30 overexpression partially restores the nep1-1ts growth deficiency
malfunction
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mutations in Nep1 result in decreased methyl donor binding, but do not result in lethality
malfunction
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Nep1 mutation D86G causes the Bowen-Conradi syndrome, BCS, that results in severe pre and postnatal growth and psychomotor retardation, microcephaly, micrognathia, rocker bottom feet and early childhood death, overview. Human HsNep1D86G protein shows a strongly increased interaction of the monomers
malfunction
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restored growth of a nep1-1ts mutant upon addition of S-adenosylmethionine also after preventing U1191 methylation in a DElTAsnr35 mutant. Nep1 methyltransferase activity is not affected upon introduction of the Bowen-Conradi syndrome, BCS, D86G mutation. Instead, the mutated protein shows enhanced dimerization propensity and increased affinity for its RNA-target in vitro
malfunction
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a temperature-sensitive ScNEP1ts1 allele is isolated and reveals a strongly increased sensitivity to paromomycin, a translational inhibitor which binds to RNA, indicating that ribosome biogenesis within the nucleolus is probably affected. Candida albicans and human NEP1 heterologously complement the essential phenotype in a Saccharomyces cerevisiae nep1 deletion mutant, the ScNEP1 spindle/microtubule phenotype is not found with HsNEP1 and CaNEP1
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malfunction
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addition of SAM to the medium restores growth at elevated temperatures in yeast with temperature sensitive mutants of the yeast Nep1 protein
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Nep1 interacting genes correspond to ribosome biogenesis, i.e. RPS18A, RPS18B, RRP8, EFG1, UTP30, to ribosome quality control, i.e. UBP3, BRE5, UBP6, and to ribosome functional control, i.e. DOM34, no-go decay
metabolism
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replacement of U1191 by any other base caused significant growth deficiencies
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MjNep1 binds its RNA target along an extended basic surface cleft at the dimer interface that involves both insertions to the SPOUT-class core fold in an orientation consistent with the proposed methylation of nt 914 in Methanocaldococcus jannaschii 16S rRNA
physiological function
Nep1 is a genuine rRNA methyltransferase and is essential for ribosome biogenesis
physiological function
Nep1 is a SPOUT RNA methyltransferase, and can catalyze methylation at the N1 of pseudouridine, it is required for 18S rRNA maturation. Nep1 is also involved in assembly of Rps19, an SSU ribosomal protein. Functional mechanism of Nep1/Emg1 N1-specific pseudouridine methyltransferase in ribosome biogenesis, structure-function relationship, overview
physiological function
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Nep1 is a SPOUT RNA methyltransferase, and can catalyze methylation at the N1 of pseudouridine, it is required for 18S rRNA maturation. Nep1 is also involved in assembly of Rps19, an SSU ribosomal protein. Functional mechanism of Nep1/Emg1 N1-specific pseudouridine methyltransferase in ribosome biogenesis, structure-function relationship, overview. Nep1 recognizes its RNA site via base-specific interactions and stabilizes a stem-loop in the bound RNA. Nep1 changes rRNA structure upon binding, a uridine base is bound in the active site of Nep1, positioned for a methyltransfer at the C5 position
physiological function
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Nep1 is essential for psi119 pseudouridine methylation, but is not required for acp-modification. Nep1 has a dual function, as psi1191-methyltransferase and ribosome assembly factor
physiological function
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the nucleolar essential protein Nep1 is an important trans-acting factor in the 90S preribosome. Nep1 methylates the hypermodified psi1191 base of 18S rRNA and has an additional essential function during ribosome biogenesis, i.e. in 40S subunit synthesis. Utp30 and Nep1 act together during pre-ribosomal complex formation and, along with Rps18, provide the surface for the Rps19 assembly to the 90S pre-ribosome
physiological function
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the rRNA modifications are thought to play a role in modulation of the three-dimensional structure of RNA and in fine-tuning its interactions with other RNAs or proteins
physiological function
the rRNA modifications are thought to play a role in modulation of the three-dimensional structure of RNA and in fine-tuning its interactions with other RNAs or proteins
physiological function
the rRNA modifications are thought to play a role in modulation of the three-dimensional structure of RNA and in fine-tuning its interactions with other RNAs or proteins
physiological function
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the rRNA modifications are thought to play a role in modulation of the three-dimensional structure of RNA and in fine-tuning its interactions with other RNAs or proteins. The protein has an essential function in ribosomal biogenesis which directly or indirectly interferes with a methylation reaction during the early steps of pre-rRNA processing necessary for the generation of 40S ribosomal subunits
physiological function
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the rRNA modifications are thought to play a role in modulation of the three-dimensional structure of RNA and in fine-tuning its interactions with other RNAs or proteins
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physiological function
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the rRNA modifications are thought to play a role in modulation of the three-dimensional structure of RNA and in fine-tuning its interactions with other RNAs or proteins. The protein has an essential function in ribosomal biogenesis which directly or indirectly interferes with a methylation reaction during the early steps of pre-rRNA processing necessary for the generation of 40S ribosomal subunits
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physiological function
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Nep1 is a genuine rRNA methyltransferase and is essential for ribosome biogenesis
-
physiological function
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the rRNA modifications are thought to play a role in modulation of the three-dimensional structure of RNA and in fine-tuning its interactions with other RNAs or proteins
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active site structure and ligand binding, overview
additional information
Candida albicans NEP1 heterologously complements the essential phenotype in a Saccharomyces cerevisiae nep1 deletion mutant
additional information
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Candida albicans NEP1 heterologously complements the essential phenotype in a Saccharomyces cerevisiae nep1 deletion mutant
additional information
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genome-wide yeast screen to uncover synthetic interactions of DELTAnep1
additional information
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human NEP1 heterologously complements the essential phenotype in a Saccharomyces cerevisiae nep1 deletion mutant
additional information
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active site structure and ligand binding, overview
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additional information
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Candida albicans NEP1 heterologously complements the essential phenotype in a Saccharomyces cerevisiae nep1 deletion mutant
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