2.1.1.260: rRNA small subunit pseudouridine methyltransferase Nep1
This is an abbreviated version!
For detailed information about rRNA small subunit pseudouridine methyltransferase Nep1, go to the full flat file.
Word Map on EC 2.1.1.260
-
2.1.1.260
-
rrnas
-
jannaschii
-
methanocaldococcus
-
nucleolus
-
hypermodified
-
s-adenosylmethionine
-
archaea
-
three-hybrid
-
rna-binding
- 2.1.1.260
- rrnas
- jannaschii
-
methanocaldococcus
- nucleolus
-
hypermodified
- s-adenosylmethionine
- archaea
-
three-hybrid
-
rna-binding
Reaction
Synonyms
Bowen-Conradi syndrome protein, Emg1, Emg1p, essential for mitotic growth 1, N1-specific pseudouridine methyltransferase, Nep1, Nep1 methyltransferase, Nep1p, nucleolar essential protein 1, pseudouridine N1-methyltransferase, pseudouridine-N1-specific methyltransferase
ECTree
2.1.1.260 rRNA small subunit pseudouridine methyltransferase Nep1Advanced search results
results (
results (Engineering
Engineering on EC 2.1.1.260 - rRNA small subunit pseudouridine methyltransferase Nep1
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
top
PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
D90G
-
a mutation in yeast Nep1 equivalent to the Bowen-Conradi syndrome, BCS, mutation in the human Nep1. Nep1 methyltransferase activity is not affected upon introduction of the BCS mutation, the mutated protein shows enhanced dimerization propensity and increased affinity for its RNA-target in vitro
additional information
additional information
construction of a heterozygous CaDnep1/CaNEP1 strain CAE8 after replacement of one CaNEP1 wild-type allele with a CaURA3 marker and introduction of GFP-open reading frame driven by the methionine/cysteine-repressible CaMET3 promoter in front of the gene. Without high concentrations of methionine and cysteine in the medium, the resulting strain is viable, but addition of 2.5 mM methionine and 2.5 mM cysteine strongly impairs growth
additional information
-
construction of a heterozygous CaDnep1/CaNEP1 strain CAE8 after replacement of one CaNEP1 wild-type allele with a CaURA3 marker and introduction of GFP-open reading frame driven by the methionine/cysteine-repressible CaMET3 promoter in front of the gene. Without high concentrations of methionine and cysteine in the medium, the resulting strain is viable, but addition of 2.5 mM methionine and 2.5 mM cysteine strongly impairs growth
additional information
-
construction of a heterozygous CaDnep1/CaNEP1 strain CAE8 after replacement of one CaNEP1 wild-type allele with a CaURA3 marker and introduction of GFP-open reading frame driven by the methionine/cysteine-repressible CaMET3 promoter in front of the gene. Without high concentrations of methionine and cysteine in the medium, the resulting strain is viable, but addition of 2.5 mM methionine and 2.5 mM cysteine strongly impairs growth
-
additional information
-
complementation of a ScDnep1 strain with the human Nep1 (C2F) protein, the HsNEP1 open reading frame is expressed with the yeast inducible/repressible GAL1 promoter and the resulting plasmid (pGALHsNEP1) is transformed into the heterozygous ScDnep1/ScNEP1 strain CEN.SR679. After sporulation and tetrad analysis, ScDnep1 segregants complemented by the pGAL-HsNEP1 are only viable with galactose
additional information
-
complementation of a ScDnep1 strain with the human Nep1 (C2F) protein, the HsNEP1 open reading frame is expressed with the yeast inducible/repressible GAL1 promoter and the resulting plasmid (pGALHsNEP1) is transformed into the heterozygous ScDnep1/ScNEP1 strain CEN.SR679. After sporulation and tetrad analysis, ScDnep1 segregants complemented by the pGAL-HsNEP1 are only viable with galactose
additional information
-
lethal phenotype of a DELTAnep1 deletion, overexpression of RPS19B and also deletions within genes DELTAnop6 and DELTAtma23, respectively, which both encode small, positively charged fungal-specific proteins, suppress the DELTAnep1 growth deficiency. Identification of DELTanep1-specific genetic interactions, polysome profiles and phenotypes, overview. DELTAAutp30 enhances the phenotype of DELTAnep1 and is a multi-copy suppressor
additional information
-
complementation of a ScDnep1 strain with the human Nep1 (C2F) protein, the HsNEP1 open reading frame is expressed with the yeast inducible/repressible GAL1 promoter and the resulting plasmid (pGALHsNEP1) is transformed into the heterozygous ScDnep1/ScNEP1 strain CEN.SR679. After sporulation and tetrad analysis, ScDnep1 segregants complemented by the pGAL-HsNEP1 are only viable with galactose
-
additional information
construction of a heterozygous CaDnep1/CaNEP1 strain CAE8 after replacement of one CaNEP1 wild-type allele with a CaURA3 marker and introduction of GFP-open reading frame driven by the methionine/cysteine-repressible CaMET3 promoter in front of the gene. Without high concentrationsof methionine and cysteine in the medium, the resulting strain is viable, but addition of 2.5 mM methionine and 2.5 mM cysteine strongly impairs growth
additional information
-
construction of a heterozygous CaDnep1/CaNEP1 strain CAE8 after replacement of one CaNEP1 wild-type allele with a CaURA3 marker and introduction of GFP-open reading frame driven by the methionine/cysteine-repressible CaMET3 promoter in front of the gene. Without high concentrationsof methionine and cysteine in the medium, the resulting strain is viable, but addition of 2.5 mM methionine and 2.5 mM cysteine strongly impairs growth
-
