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2.1.1.260: rRNA small subunit pseudouridine methyltransferase Nep1

This is an abbreviated version!
For detailed information about rRNA small subunit pseudouridine methyltransferase Nep1, go to the full flat file.

Word Map on EC 2.1.1.260

Reaction

S-adenosyl-L-methionine
+
pseudouridine1191 in yeast 18S rRNA
=
S-adenosyl-L-homocysteine
+
N1-methylpseudouridine1191 in yeast 18S rRNA

Synonyms

Bowen-Conradi syndrome protein, Emg1, Emg1p, essential for mitotic growth 1, N1-specific pseudouridine methyltransferase, Nep1, Nep1 methyltransferase, Nep1p, nucleolar essential protein 1, pseudouridine N1-methyltransferase, pseudouridine-N1-specific methyltransferase

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.260 rRNA small subunit pseudouridine methyltransferase Nep1

Cloned

Cloned on EC 2.1.1.260 - rRNA small subunit pseudouridine methyltransferase Nep1

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression of His6-tagged Nep1 in Escherichia coli strain RosettaTM-DE3-pLysS
-
expression of wild-type and selenomethionine-labeled His6-tagged Nep1 in Escherichia coli strain RosettaTM-DE3-pLysS
gene NEP1, DNA and amino acid sequence determination and analysis
gene NEP1, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3)
gene NEP1, DNA and amino acid sequence determination and analysis, sequence comparisons, expression of the GFP-tagged ScNEP1 in enzyme-deficient Saccharomyces cerevisiae strain CEN.SR679 in the nucleus
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gene NEP1, expression of N-terminally His6-tagged wild-type and D90G mutant Nep1 in Escherichia coli strain XL1-blue
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gene NEP1, recombinaant expression of Nep1
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gene NEP1, recombinant expression of His-tagged Nep1 in Escherichia coli strain M15(pREP4)
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gene NEP1, stable expression of GFP-tagged wild-type and D86G mutant Nep1 in 293T cells under control of the tetracycline-inducible promoter, expression of N-terminally His6-tagged wild-type and D90G mutant Nep1 in Escherichia coli strain XL1-blue
-