2.1.1.260: rRNA small subunit pseudouridine methyltransferase Nep1
This is an abbreviated version!
For detailed information about rRNA small subunit pseudouridine methyltransferase Nep1, go to the full flat file.
Word Map on EC 2.1.1.260
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2.1.1.260
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rrnas
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jannaschii
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methanocaldococcus
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nucleolus
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hypermodified
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s-adenosylmethionine
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archaea
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three-hybrid
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rna-binding
- 2.1.1.260
- rrnas
- jannaschii
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methanocaldococcus
- nucleolus
-
hypermodified
- s-adenosylmethionine
- archaea
-
three-hybrid
-
rna-binding
Reaction
Synonyms
Bowen-Conradi syndrome protein, Emg1, Emg1p, essential for mitotic growth 1, N1-specific pseudouridine methyltransferase, Nep1, Nep1 methyltransferase, Nep1p, nucleolar essential protein 1, pseudouridine N1-methyltransferase, pseudouridine-N1-specific methyltransferase
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Natural Substrates Products
Natural Substrates Products on EC 2.1.1.260 - rRNA small subunit pseudouridine methyltransferase Nep1
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REACTION DIAGRAM
S-adenosyl-L-methionine + pseudouridine1191 in yeast 18S rRNA
S-adenosyl-L-homocysteine + N1-methylpseudouridine1191 in yeast 18S rRNA
additional information
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Nep1 target site identification by mass spectrometry, isotope-labeling, and fluorescence anisotropy measurements
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S-adenosyl-L-homocysteine + N1-methylpseudouridine1191 in yeast 18S rRNA
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S-adenosyl-L-methionine + pseudouridine1191 in yeast 18S rRNA
S-adenosyl-L-homocysteine + N1-methylpseudouridine1191 in yeast 18S rRNA
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S-adenosyl-L-methionine + pseudouridine1191 in yeast 18S rRNA
S-adenosyl-L-homocysteine + N1-methylpseudouridine1191 in yeast 18S rRNA
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?
S-adenosyl-L-methionine + pseudouridine1191 in yeast 18S rRNA
S-adenosyl-L-homocysteine + N1-methylpseudouridine1191 in yeast 18S rRNA
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-
-
?
S-adenosyl-L-methionine + pseudouridine1191 in yeast 18S rRNA
S-adenosyl-L-homocysteine + N1-methylpseudouridine1191 in yeast 18S rRNA
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-
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?
S-adenosyl-L-methionine + pseudouridine1191 in yeast 18S rRNA
S-adenosyl-L-homocysteine + N1-methylpseudouridine1191 in yeast 18S rRNA
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?
S-adenosyl-L-methionine + pseudouridine1191 in yeast 18S rRNA
S-adenosyl-L-homocysteine + N1-methylpseudouridine1191 in yeast 18S rRNA
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Nep1 catalyzes the psi1191 methylation in vivo. The in vivo target site for Nep1-catalyzed methylation is located within loop 35 of the 18S rRNA that contains the unique hypermodification of U1191 to 1-methyl-3-(3-amino-3-carboxypropyl)-pseudouridine
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S-adenosyl-L-methionine + pseudouridine1191 in yeast 18S rRNA
S-adenosyl-L-homocysteine + N1-methylpseudouridine1191 in yeast 18S rRNA
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