2.1.1.260: rRNA small subunit pseudouridine methyltransferase Nep1

This is an abbreviated version!
For detailed information about rRNA small subunit pseudouridine methyltransferase Nep1, go to the full flat file.

Reaction

Synonyms

Bowen-Conradi syndrome protein, Emg1, Emg1p, essential for mitotic growth 1, N1-specific pseudouridine methyltransferase, Nep1, Nep1 methyltransferase, Nep1p, nucleolar essential protein 1, pseudouridine N1-methyltransferase, pseudouridine-N1-specific methyltransferase

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.1 Methyltransferases

                2.1.1.260 rRNA small subunit pseudouridine methyltransferase Nep1

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Results	in table
44	AA Sequence
10	Cloned(Commentary)
10	Cofactor
3	Crystallization (Commentary)
1	Expression
35	General Information
1	Inhibitors
12	Localization
2	Molecular Weight [Da]
12	Natural Substrates/ Products (Substrates)
17	Organism
1	Pathway
51	PDB ID
4	pH Optimum
10	Protein Variants
8	Purification (Commentary)
1	Reaction
16	Reference
1	Source Tissue
21	Substrates and Products (Substrate)
9	Subunits
56	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]

Crystallization

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Crystallization on EC 2.1.1.260 - rRNA small subunit pseudouridine methyltransferase Nep1

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

recombinant detagged wild-type and selenomethionine-labeled afNep1 dimer bound to S-adenosyl homocysteine, X-ray diffraction structure determination and analysis at 1.45-2.0 A resolution

Archaeoglobus fulgidus

O29524

720589

Nep1 in its free form and bound to S-adenosylhomocysteine or the antibiotic and general methyltransferase inhibitor sinefungin, hanging drop vapour diffusion method, 10-15 mg/ml protein in 100 mM BisTris-propane buffer, pH 9.5, 6-10% PEG 400, 30-48% glycerol, and 0-400 mM trimethylamine-N-oxide, at 4°C, crystals of the selenomethionine containing protein in the presence or absence of a 3fold excess of S-adenosyl-L-homocysteine are grown under similar conditions at 16°C, X-ray diffraction structure determination and analysis at 2.2 A, 2.15 A, and 2.25 A resolution, respectively

Methanocaldococcus jannaschii

Q57977

720579

recombinant detagged scNep1 dimer bound to S-adenosyl homocysteine and in complexes with RNA, i.e. one molecule and two molecules of cognate RNA, X-ray diffraction structure determination and analysis at 1.80-1.90 A and at 3.00 A resolution, respectively

Saccharomyces cerevisiae

-

720589