1.21.3.1: isopenicillin-N synthase

This is an abbreviated version!
For detailed information about isopenicillin-N synthase, go to the full flat file.

Reaction

Synonyms

IPN cyclase, IPN synthase, IPNS, isopenicillin N synthase, isopenicillin N synthase (cyclase), isopenicillin N synthetase, isopenicillin N-synthase, isopenicillin-N synthase, isopenicillin-N-synthase, More, PcbC, synthetase, isopenicillin N (9Cl)

ECTree

     1 Oxidoreductases

         1.21 Catalysing the reaction X-H + Y-H = X-Y

             1.21.3 With oxygen as acceptor

                1.21.3.1 isopenicillin-N synthase

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
32	Activating Compound
57	AA Sequence
5	Application
1	CAS Registry Number
14	Cloned(Commentary)
13	Crystallization (Commentary)
3	Expression
2	General Information
1	General Stability
52	Inhibitors
1	Ki Value [mM]
6	KM Value [mM]
11	Localization
30	Metals/Ions
4	Molecular Weight [Da]
66	Natural Substrates/ Products (Substrates)
93	Organism
1	Oxidation Stability
4	Pathway
45	PDB ID
3	pH Optimum
2	pH Range
51	Protein Variants
12	Purification (Commentary)
17	Reaction
32	Reaction Type
69	Reference
1	Renatured (Commentary)
1	Source Tissue
17	Specific Activity [micromol/min/mg]
123	Substrates and Products (Substrate)
6	Subunits
104	Synonyms
1	Systematic Name
7	Temperature Optimum [°C]
1	Temperature Range [°C]
1	Turnover Number [1/s]

Natural Substrates Products

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

Natural Substrates Products on EC 1.21.3.1 - isopenicillin-N synthase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide show all columns Go to Natural Substrate Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

adipyl-L-cysteinyl-D-valine + ?

N-(4-carboxybutyl)penicillin + 2 H2O

Actinomyces sp.

-

-

700004

-

-

?

delta-L-alpha-aminoadipoyl-L-cysteinyl-D-valine + O2

isopenicillin N + 2 H2O

Aspergillus nidulans

P05326

-

697317

-

-

?

L-alpha-aminoadipoyl-L-cysteinyl-D-valine + O2

? + H2O

Aspergillus nidulans

-

-

696215

-

-

?

L-alpha-aminoadipoyl-L-cysteinyl-D-valine + O2

isopenicillin N + 2 H2O

Aspergillus nidulans

-

-

698457

-

-

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin + H2O

Streptomyces microflavus

-

-

687292

-

-

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + 2 H2O

34 entries

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + H2O

23 entries

phenylacetyl-L-cysteinyl-D-valine + O2

penicillin G + 2 H2O

Actinomyces sp.

-

-

700004

-

-

?

additional information

?

-

Penicillium chrysogenum

-

in Penicillium chrysogenum, the enzymes involved in penicillin production are compartmentalized in the cytosol and in microbodies

685812

-

-

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + 2 H2O

Acremonium chrysogenum

-

common step in the biosynthesis of penicillins, cephalosporins and cephamycins

440317

-

440317

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + 2 H2O

Acremonium chrysogenum

-

common step in the biosynthesis of penicillins, cephalosporins and cephamycins

440318

-

440318

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + 2 H2O

Acremonium chrysogenum

-

common step in the biosynthesis of penicillins, cephalosporins and cephamycins

440319

-

-

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + 2 H2O

Acremonium chrysogenum

-

common step in the biosynthesis of penicillins, cephalosporins and cephamycins

440324

-

440324

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + 2 H2O

Acremonium chrysogenum

-

common step in the biosynthesis of penicillins, cephalosporins and cephamycins

440329

-

-

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + 2 H2O

Acremonium chrysogenum

-

common step in the biosynthesis of penicillins, cephalosporins and cephamycins

440314

product has antibiotic activity

440314

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + 2 H2O

Acremonium chrysogenum

-

common step in the biosynthesis of penicillins, cephalosporins and cephamycins

440323

product has antibiotic activity

440323

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + 2 H2O

Acremonium chrysogenum

-

modifications of the L-cysteine residue in the second position of the enzyme result in tripeptides that are unable to serve as substrates

440318

-

440318

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + 2 H2O

Acremonium chrysogenum

-

key enzyme of the biosynthetic pathway

440323

product has antibiotic activity

440323

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + 2 H2O

Acremonium chrysogenum

-

involved in biosynthesis of cephalosporin C

492265

-

-

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + 2 H2O

Acremonium chrysogenum C10 / ATCC 48272

-

involved in biosynthesis of cephalosporin C

492265

-

-

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + 2 H2O

Actinomyces sp.

-

IPNSs are nonheme iron-dependent oxygenases that catalyze cyclization

700004

-

-

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + 2 H2O

Amycolatopsis lactamdurans

-

common step in the biosynthesis of penicillins, cephalosporins and cephamycins

440314

product has antibiotic activity

440314

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + 2 H2O

Aspergillus nidulans

-

catalytic reaction is under steric regulation

440325

product has antibiotic activity

440325

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + 2 H2O

Aspergillus nidulans

-

common step in the biosynthesis of penicillins, cephalosporins and cephamycins

440327

-

-

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + 2 H2O

Aspergillus nidulans

P05326

common step in the biosynthesis of penicillins, cephalosporins and cephamycins

440323

product has antibiotic activity

440323

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + 2 H2O

Aspergillus nidulans

-

common step in the biosynthesis of penicillins, cephalosporins and cephamycins

440325

product has antibiotic activity

440325

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + 2 H2O

Aspergillus nidulans

P05326

common step in the biosynthesis of penicillins, cephalosporins and cephamycins

440320

product has antibiotic activity

440320

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + 2 H2O

Aspergillus nidulans

P05326

common step in the biosynthesis of penicillins, cephalosporins and cephamycins

440322

product has antibiotic activity

440322

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + 2 H2O

Aspergillus nidulans

P05326

key enzyme of the biosynthetic pathway

440323

product has antibiotic activity

440323

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + 2 H2O

Flavobacterium sp.

-

common step in the biosynthesis of penicillins, cephalosporins and cephamycins

440316

product has antibiotic activity

440316

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + 2 H2O

Flavobacterium sp. SC 12.154

-

common step in the biosynthesis of penicillins, cephalosporins and cephamycins

440316

product has antibiotic activity

440316

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + 2 H2O

Penicillium chrysogenum

-

-

440330

-

-

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + 2 H2O

Penicillium chrysogenum

-

common step in the biosynthesis of penicillins, cephalosporins and cephamycins

440318

-

440318

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + 2 H2O

Penicillium chrysogenum

-

common step in the biosynthesis of penicillins, cephalosporins and cephamycins

440314

product has antibiotic activity

440314

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + 2 H2O

Penicillium chrysogenum

-

modifications of the L-cysteine residue in the second position of the enzyme result in tripeptides that are unable to serve as substrates

440318

-

440318

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + 2 H2O

Streptomyces clavuligerus

-

common step in the biosynthesis of penicillins, cephalosporins and cephamycins

440315

product has antibiotic activity

440315

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + 2 H2O

Streptomyces clavuligerus

-

common step in the biosynthesis of penicillins, cephalosporins and cephamycins

440321

product has antibiotic activity

440321

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + 2 H2O

Streptomyces clavuligerus

-

evaluation of culture conditions for penicillin and cephalosporin C production

440315

product has antibiotic activity

440315

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + 2 H2O

Streptomyces jumonjinensis

-

-

440328

-

-

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + 2 H2O

Streptomyces jumonjinensis

-

common step in the biosynthesis of penicillins, cephalosporins and cephamycins

440323

product has antibiotic activity

440323

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + 2 H2O

Streptomyces jumonjinensis

-

key enzyme of the biosynthetic pathway

440323

product has antibiotic activity

440323

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + 2 H2O

Streptomyces microflavus

-

common step in the biosynthesis of penicillins, cephalosporins and cephamycins

440326

-

-

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + 2 H2O

Streptomyces microflavus NRRL 3584

-

common step in the biosynthesis of penicillins, cephalosporins and cephamycins

440326

-

-

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + H2O

Acremonium chrysogenum

-

-

745018

-

-

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + H2O

Acremonium chrysogenum

P05189

-

746185

-

-

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + H2O

Acremonium chrysogenum 3.3795

-

-

745018

-

-

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + H2O

Amycolatopsis lactamdurans

P27744

-

746185

-

-

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + H2O

Aspergillus nidulans

-

-

745172

-

-

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + H2O

Aspergillus nidulans

P05326

-

744719, 746185

-

-

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + H2O

Aspergillus nidulans ATCC 38163

P05326

-

744719, 746185

-

-

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + H2O

Crassostrea gigas

K1QF46

-

746185

-

-

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + H2O

Flavobacterium sp. SC 12154

P16020

-

746185

-

-

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + H2O

Gordonia rubripertincta

-

-

746185

-

-

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + H2O

Gordonia rubripertincta NBRC 101908

-

-

746185

-

-

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + H2O

Lysobacter lactamgenus

Q48739

-

746185

-

-

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + H2O

Mycolicibacterium phlei

-

-

746185

-

-

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + H2O

Mycolicibacterium phlei RIVM601174

-

-

746185

-

-

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + H2O

Penicillium chrysogenum

-

-

746275

-

-

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + H2O

Penicillium chrysogenum

P08703

-

746185

-

-

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + H2O

Penicillium chrysogenum PQ-96

-

-

746275

-

-

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + H2O

Streptomyces cattleya

Q53932

-

746185

-

-

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + H2O

Streptomyces clavuligerus

P10621

-

746185

-

-

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + H2O

Streptomyces clavuligerus ATCC 27064

P10621

-

746185

-

-

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + H2O

Streptomyces griseus

Q54243

-

746185

-

-

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + H2O

Streptomyces jumonjinensis

P18286

-

746185

-

-

?

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2

isopenicillin N + H2O

Streptomyces microflavus

P12438

-

746185

-

-

?