Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant in Escherichia coli, evaluation of growth temperature for expression of the soluble mutant and wild-type in the Escherichia coli host BL21 (DE3) | Acremonium chrysogenum |
Protein Variants | Comment | Organism |
---|---|---|
P285L | site-directed mutagenesis, complete loss of activity, increased soluble expression in Escherichia coli host | Acremonium chrysogenum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | Acremonium chrysogenum | common step in the biosynthesis of penicillins, cephalosporins and cephamycins | isopenicillin N + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acremonium chrysogenum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | - |
Acremonium chrysogenum | isopenicillin N + 2 H2O | - |
? | |
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | common step in the biosynthesis of penicillins, cephalosporins and cephamycins | Acremonium chrysogenum | isopenicillin N + 2 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IPNS | - |
Acremonium chrysogenum |
isopenicillin N-synthase | - |
Acremonium chrysogenum |