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1.21.3.1: isopenicillin-N synthase

This is an abbreviated version!
For detailed information about isopenicillin-N synthase, go to the full flat file.

Word Map on EC 1.21.3.1

Reaction

N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine
+
O2
=
isopenicillin N
 + 2 H2O

Synonyms

IPN cyclase, IPN synthase, IPNS, isopenicillin N synthase, isopenicillin N synthase (cyclase), isopenicillin N synthetase, isopenicillin N-synthase, isopenicillin-N synthase, isopenicillin-N-synthase, More, PcbC, synthetase, isopenicillin N (9Cl)

ECTree

     1 Oxidoreductases
         1.21 Catalysing the reaction X-H + Y-H = X-Y
             1.21.3 With oxygen as acceptor
                1.21.3.1 isopenicillin-N synthase

Cloned

Cloned on EC 1.21.3.1 - isopenicillin-N synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
-
expressed in Escherichia coli BL21(DE3) cells
-
expression in Escherichia coli
expression of wild-type and mutant in Escherichia coli, evaluation of growth temperature for expression of the soluble mutant and wild-type in the Escherichia coli host BL21 (DE3)
-
expression of wild-type and mutants as maltose-binding fusion proteins in Escherichia coli
-
expression of wild-type and mutants in Escherichia coli
-
expression of wild-type and mutants in Escherichia coli, amino acid sequence comparison
-
functional expression in the cytosol of yeast Hansenula polymorpha, best at 25°C or 30°C growth temperature, at 37°C the recombinant protein is not stable, optimization of culture conditions, overview
-
overexpression in Escherichia coli K12 strain JM109 in inclusion bodies
-
overexpression in Escherichia coli, DNA sequence analysis
-
overexpression of recombinant enzyme mutants in Cephalosporium acremonium
-