Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli, DNA sequence analysis | Streptomyces microflavus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37800 | - |
x * 37800, DNA sequence determination | Streptomyces microflavus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | Streptomyces microflavus | common step in the biosynthesis of penicillins, cephalosporins and cephamycins | isopenicillin N + 2 H2O | - |
? | |
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | Streptomyces microflavus NRRL 3584 | common step in the biosynthesis of penicillins, cephalosporins and cephamycins | isopenicillin N + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces microflavus | - |
- |
- |
Streptomyces microflavus NRRL 3584 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000000093 | - |
purified recombinant enzyme | Streptomyces microflavus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | - |
Streptomyces microflavus | isopenicillin N + 2 H2O | - |
? | |
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | common step in the biosynthesis of penicillins, cephalosporins and cephamycins | Streptomyces microflavus | isopenicillin N + 2 H2O | - |
? | |
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | - |
Streptomyces microflavus NRRL 3584 | isopenicillin N + 2 H2O | - |
? | |
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | common step in the biosynthesis of penicillins, cephalosporins and cephamycins | Streptomyces microflavus NRRL 3584 | isopenicillin N + 2 H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37800, DNA sequence determination | Streptomyces microflavus |
Synonyms | Comment | Organism |
---|---|---|
IPNS | - |
Streptomyces microflavus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
26 | - |
assay at | Streptomyces microflavus |