7.6.2.8: ABC-type vitamin B12 transporter
This is an abbreviated version!
For detailed information about ABC-type vitamin B12 transporter, go to the full flat file.
Word Map on EC 7.6.2.8
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7.6.2.8
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transcobalamin
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azurophilic
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cyanocobalamin
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lmbrd1
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outward-facing
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cobinamide
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inward-facing
- 7.6.2.8
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transcobalamin
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azurophilic
- cyanocobalamin
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lmbrd1
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outward-facing
- cobinamide
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inward-facing
Reaction
Synonyms
ABC transporter, ABCD4, BtuC, BtuCD, BtuCD vitamin B12 importer, BtuCD-BtuF, BtuCD-F, BtuCD-F complex, BtuD, BtuF, BtuF-CD, BtuM, BtuMTd, Cbl transporter, cobalamin-specific ECF-type ABC transporter, EC 3.6.3.33, ECF-CbrT, ECF-type ABC transporter for vitamin B12, energy coupling factor (ECF-) type ABC transporter, Rv1819c, Tbd_2719, vitamin B12 ABC importer, vitamin B12 import ATP-binding protein, vitamin B12 import system, vitamin B12 import system permease protein, vitamin B12 importer, vitamin B12 transporter, vitamin B12-binding protein, vitamin B12-transporting ATPase
ECTree
7.6.2.8 ABC-type vitamin B12 transporterAdvanced search results
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results (Natural Substrates Products
Natural Substrates Products on EC 7.6.2.8 - ABC-type vitamin B12 transporter
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NATURAL SUBSTRATE 
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
ATP + H2O + cyanocobalamin-[cobalamin-binding protein][side 1]
ADP + phosphate + cyanocobalamin[side 2] + [cobalamin-binding protein][side 1]
ATP + H2O + dicyanocobinamide-[cobalamin-binding protein][side 1]
ADP + phosphate + dicyanocobinamide[side 2] + [cobalamin-binding protein][side 1]
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
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?
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
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-
-
?
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
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-
-
?
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
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-
-
?
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
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-
-
?
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
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-
-
?
ATP + H2O + cobinamide-[cobalamin-binding protein][side 1]
ADP + phosphate + cobinamide[side 2] + [cobalamin-binding protein][side 1]
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-
-
?
ATP + H2O + cyanocobalamin-[cobalamin-binding protein][side 1]
ADP + phosphate + cyanocobalamin[side 2] + [cobalamin-binding protein][side 1]
A0A0H3AMA6; A5F1V0; A5F5P5
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-
-
?
ATP + H2O + cyanocobalamin-[cobalamin-binding protein][side 1]
ADP + phosphate + cyanocobalamin[side 2] + [cobalamin-binding protein][side 1]
A0A0H3AMA6; A5F1V0; A5F5P5
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-
-
?
ATP + H2O + cyanocobalamin-[cobalamin-binding protein][side 1]
ADP + phosphate + cyanocobalamin[side 2] + [cobalamin-binding protein][side 1]
A0A0H3AMA6; A5F1V0; A5F5P5
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-
-
?
ATP + H2O + cyanocobalamin-[cobalamin-binding protein][side 1]
ADP + phosphate + cyanocobalamin[side 2] + [cobalamin-binding protein][side 1]
A0A0H3AMA6; A5F1V0; A5F5P5
-
-
-
?
ATP + H2O + dicyanocobinamide-[cobalamin-binding protein][side 1]
ADP + phosphate + dicyanocobinamide[side 2] + [cobalamin-binding protein][side 1]
A0A0H3AMA6; A5F1V0; A5F5P5
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-
-
?
ATP + H2O + dicyanocobinamide-[cobalamin-binding protein][side 1]
ADP + phosphate + dicyanocobinamide[side 2] + [cobalamin-binding protein][side 1]
A0A0H3AMA6; A5F1V0; A5F5P5
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-
-
?
ATP + H2O + dicyanocobinamide-[cobalamin-binding protein][side 1]
ADP + phosphate + dicyanocobinamide[side 2] + [cobalamin-binding protein][side 1]
A0A0H3AMA6; A5F1V0; A5F5P5
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-
-
?
ATP + H2O + dicyanocobinamide-[cobalamin-binding protein][side 1]
ADP + phosphate + dicyanocobinamide[side 2] + [cobalamin-binding protein][side 1]
A0A0H3AMA6; A5F1V0; A5F5P5
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-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
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-
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?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
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-
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
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-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
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-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
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-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
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-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
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-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
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-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
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-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
-
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
A0A0H3AMA6; A5F1V0; A5F5P5
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-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
A0A0H3AMA6; A5F1V0; A5F5P5
-
-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
A0A0H3AMA6; A5F1V0; A5F5P5
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-
-
?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]
A0A0H3AMA6; A5F1V0; A5F5P5
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-
-
?
ATP + H2O + vitamin B12/out
ADP + phosphate + vitamin B12/in
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-
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-
?
ATP + H2O + vitamin B12/out
ADP + phosphate + vitamin B12/in
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-
-
?
ATP + H2O + vitamin B12/out
ADP + phosphate + vitamin B12/in
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-
-
?
ATP + H2O + vitamin B12/out
ADP + phosphate + vitamin B12/in
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-
-
?
ATP + H2O + vitamin B12/out
ADP + phosphate + vitamin B12/in
BtuCD is a type II ABC importer that catalyzes the translocation of vitamin B12 from the periplasm into the cytoplasm of Escherichia coli. BtuD is complexed with BtuC, a permease protein, and BtuF, a periplasmic binding protein, structure, overview
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-
?
ATP + H2O + vitamin B12/out
ADP + phosphate + vitamin B12/in
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-
-
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?
ATP + H2O + vitamin B12/out
ADP + phosphate + vitamin B12/in
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-
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?
ATP + H2O + vitamin B12/out
ADP + phosphate + vitamin B12/in
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-
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?
additional information
?
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BtuCD alone or the BtuCD-F complex do not bind vitamin B12. Only free, uncomplexed BtuF binds vitamin B12 with high affinity. Upon binding of BtuF to BtuCD, vitamin B12 is released from BtuF and is only transiently associated with the complex
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?
additional information
?
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BtuCD forms a stable complex with the vitamin B12 binding protein BtuF. Using protein docking and MD simulation studies it is shown that holo-BtuF stabilizes the open conformation of BtuCD, whereas the transporter begins to close again when BtuF or vitamin B12 is removed suggesting BtuCD-F is capable of substrate sensitivity. BtuC transmembrane helices 3 and 5, the L-loops and the adjacent helices comprised of BtuC residues 170-180 are identified as hotspots of conformational change
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-
?
additional information
?
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BtuCD forms an extremely stable complex with the vitamin B12 binding protein BtuF. Vitamin B12 accelerates complex dissociation rate, with ATP having an additional destabilizing effecf
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?
additional information
?
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in the presence of vitamin B12, upon binding of ATP (or ATP analogues), no association between BtuF and BtuCD can be detected
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?
additional information
?
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metal-chelate-type ABC transporter HI1470/1 is homologous with vitamin B12 importer BtuCD but exhibits a distinct inward-facing conformation in contrast to the outward-facing conformation of BtuCD. The outward-facing conformation of HI1470/1 is considered to be one of the intermediate states in the translocation cycle of BtuCD
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-
?
additional information
?
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BtuM binds vitamin B12 in its base-off conformation, in which the 5,6-dimethylbenzimidazole moiety does not bind to the cobalt ion, but with a cysteine residue as axial ligand of the corrin cobalt ion, BtuMTd binds cobalamin (Cbl) using cysteine ligation. In contrast, at physiological pH the conformation of free Cbl in aqueous solution is base-on with the 5,6-dimethylbenzimidazole moiety coordinated to the cobalt ion in the alpha-axial position
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-
?
additional information
?
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A0A0H3AMA6; A5F1V0; A5F5P5
VcBtuF, the periplasmic substrate binding protein (PBP) of putative ABC transporter BtuF-CD of Vibrio cholerae O395, binds cyanocobalamin and dicyanocobinamide with micromolar dissociation constants (Kd). Productive internalization of these nutrients
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-
?
additional information
?
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A0A0H3AMA6; A5F1V0; A5F5P5
VcBtuF, the periplasmic substrate binding protein (PBP) of putative ABC transporter BtuF-CD of Vibrio cholerae O395, binds cyanocobalamin and dicyanocobinamide with micromolar dissociation constants (Kd). Productive internalization of these nutrients
-
-
?
additional information
?
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A0A0H3AMA6; A5F1V0; A5F5P5
VcBtuF, the periplasmic substrate binding protein (PBP) of putative ABC transporter BtuF-CD of Vibrio cholerae O395, binds cyanocobalamin and dicyanocobinamide with micromolar dissociation constants (Kd). Productive internalization of these nutrients
-
-
?
additional information
?
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A0A0H3AMA6; A5F1V0; A5F5P5
VcBtuF, the periplasmic substrate binding protein (PBP) of putative ABC transporter BtuF-CD of Vibrio cholerae O395, binds cyanocobalamin and dicyanocobinamide with micromolar dissociation constants (Kd). Productive internalization of these nutrients
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-
?
