Literature summary for 7.6.2.8 extracted from

Agarwal, S.; Dey, S.; Ghosh, B.; Biswas, M.; Dasgupta, J.
Mechanistic basis of vitamin B12 and cobinamide salvaging by the Vibrio species (2019), Biochim. Biophys. Acta, 1867, 140-151 .

Cloned(Commentary)

Cloned (Comment)	Organism
gene btuF, phylogenetic tree of BtuF, recombinant expression of His6-tagged BtuF in Escherichia coli strain XL1-Blue	Vibrio cholerae serotype O1

Crystallization (Commentary)

Crystallization (Comment)	Organism
vitamin B12-bound VcBtuF, protein in a solution with vitamin B12 in a 3:1 ratio, and 50 mM Tris-HCl, pH 7.0, and 300 mM NaCl, mixing of 0.003 ml of protein solution with 0.002 ml of precipitant solution containing 0.8 M ammonium sulfate, 0.1 M Tris, pH 8.0, and equilibration against a reservoir solution containing 0.5 ml of 1.6 M ammonium sulfate, 0.1 M HEPES, pH 7.0, 20°C, 7 days, hanging drop vapour diffusion method, X-ray diffraction structure determination and analysis at 1.67 A resolution	Vibrio cholerae serotype O1

Crystallization (Comment)

Organism

vitamin B12-bound VcBtuF, protein in a solution with vitamin B12 in a 3:1 ratio, and 50 mM Tris-HCl, pH 7.0, and 300 mM NaCl, mixing of 0.003 ml of protein solution with 0.002 ml of precipitant solution containing 0.8 M ammonium sulfate, 0.1 M Tris, pH 8.0, and equilibration against a reservoir solution containing 0.5 ml of 1.6 M ammonium sulfate, 0.1 M HEPES, pH 7.0, 20°C, 7 days, hanging drop vapour diffusion method, X-ray diffraction structure determination and analysis at 1.67 A resolution

Vibrio cholerae serotype O1

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	transmembrane enzyme	Vibrio cholerae serotype O1	16020	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Vibrio cholerae serotype O1

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
ATP + H2O + cyanocobalamin-[cobalamin-binding protein][side 1]	Vibrio cholerae serotype O1	-	ADP + phosphate + cyanocobalamin[side 2] + [cobalamin-binding protein][side 1]	-	?
ATP + H2O + cyanocobalamin-[cobalamin-binding protein][side 1]	Vibrio cholerae serotype O1 Classical Ogawa 395	-	ADP + phosphate + cyanocobalamin[side 2] + [cobalamin-binding protein][side 1]	-	?
ATP + H2O + cyanocobalamin-[cobalamin-binding protein][side 1]	Vibrio cholerae serotype O1 ATCC 39541	-	ADP + phosphate + cyanocobalamin[side 2] + [cobalamin-binding protein][side 1]	-	?
ATP + H2O + cyanocobalamin-[cobalamin-binding protein][side 1]	Vibrio cholerae serotype O1 O395	-	ADP + phosphate + cyanocobalamin[side 2] + [cobalamin-binding protein][side 1]	-	?
ATP + H2O + dicyanocobinamide-[cobalamin-binding protein][side 1]	Vibrio cholerae serotype O1	-	ADP + phosphate + dicyanocobinamide[side 2] + [cobalamin-binding protein][side 1]	-	?
ATP + H2O + dicyanocobinamide-[cobalamin-binding protein][side 1]	Vibrio cholerae serotype O1 Classical Ogawa 395	-	ADP + phosphate + dicyanocobinamide[side 2] + [cobalamin-binding protein][side 1]	-	?
ATP + H2O + dicyanocobinamide-[cobalamin-binding protein][side 1]	Vibrio cholerae serotype O1 ATCC 39541	-	ADP + phosphate + dicyanocobinamide[side 2] + [cobalamin-binding protein][side 1]	-	?
ATP + H2O + dicyanocobinamide-[cobalamin-binding protein][side 1]	Vibrio cholerae serotype O1 O395	-	ADP + phosphate + dicyanocobinamide[side 2] + [cobalamin-binding protein][side 1]	-	?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]	Vibrio cholerae serotype O1	-	ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]	-	?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]	Vibrio cholerae serotype O1 Classical Ogawa 395	-	ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]	-	?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]	Vibrio cholerae serotype O1 ATCC 39541	-	ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]	-	?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]	Vibrio cholerae serotype O1 O395	-	ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]	-	?
additional information	Vibrio cholerae serotype O1	VcBtuF, the periplasmic substrate binding protein (PBP) of putative ABC transporter BtuF-CD of Vibrio cholerae O395, binds cyanocobalamin and dicyanocobinamide with micromolar dissociation constants (Kd). Productive internalization of these nutrients	?	-	?
additional information	Vibrio cholerae serotype O1 Classical Ogawa 395	VcBtuF, the periplasmic substrate binding protein (PBP) of putative ABC transporter BtuF-CD of Vibrio cholerae O395, binds cyanocobalamin and dicyanocobinamide with micromolar dissociation constants (Kd). Productive internalization of these nutrients	?	-	?
additional information	Vibrio cholerae serotype O1 ATCC 39541	VcBtuF, the periplasmic substrate binding protein (PBP) of putative ABC transporter BtuF-CD of Vibrio cholerae O395, binds cyanocobalamin and dicyanocobinamide with micromolar dissociation constants (Kd). Productive internalization of these nutrients	?	-	?
additional information	Vibrio cholerae serotype O1 O395	VcBtuF, the periplasmic substrate binding protein (PBP) of putative ABC transporter BtuF-CD of Vibrio cholerae O395, binds cyanocobalamin and dicyanocobinamide with micromolar dissociation constants (Kd). Productive internalization of these nutrients	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Vibrio cholerae serotype O1	A0A0H3AMA6 AND A5F1V0 AND A5F5P5	genes btuC, btuD, and btuF encoding for vitamin B12 import system permease protein BtuC, vitamin B12 import ATP-binding protein BtuD, and vitamin B12-binding protein BtuF	-
Vibrio cholerae serotype O1 ATCC 39541	A0A0H3AMA6 AND A5F1V0 AND A5F5P5	genes btuC, btuD, and btuF encoding for vitamin B12 import system permease protein BtuC, vitamin B12 import ATP-binding protein BtuD, and vitamin B12-binding protein BtuF	-
Vibrio cholerae serotype O1 Classical Ogawa 395	A0A0H3AMA6 AND A5F1V0 AND A5F5P5	genes btuC, btuD, and btuF encoding for vitamin B12 import system permease protein BtuC, vitamin B12 import ATP-binding protein BtuD, and vitamin B12-binding protein BtuF	-
Vibrio cholerae serotype O1 O395	A0A0H3AMA6 AND A5F1V0 AND A5F5P5	genes btuC, btuD, and btuF encoding for vitamin B12 import system permease protein BtuC, vitamin B12 import ATP-binding protein BtuD, and vitamin B12-binding protein BtuF	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
ATP + H2O + cyanocobalamin-[cobalamin-binding protein][side 1]	-	Vibrio cholerae serotype O1	ADP + phosphate + cyanocobalamin[side 2] + [cobalamin-binding protein][side 1]	-	?
ATP + H2O + cyanocobalamin-[cobalamin-binding protein][side 1]	-	Vibrio cholerae serotype O1 Classical Ogawa 395	ADP + phosphate + cyanocobalamin[side 2] + [cobalamin-binding protein][side 1]	-	?
ATP + H2O + cyanocobalamin-[cobalamin-binding protein][side 1]	-	Vibrio cholerae serotype O1 ATCC 39541	ADP + phosphate + cyanocobalamin[side 2] + [cobalamin-binding protein][side 1]	-	?
ATP + H2O + cyanocobalamin-[cobalamin-binding protein][side 1]	-	Vibrio cholerae serotype O1 O395	ADP + phosphate + cyanocobalamin[side 2] + [cobalamin-binding protein][side 1]	-	?
ATP + H2O + dicyanocobinamide-[cobalamin-binding protein][side 1]	-	Vibrio cholerae serotype O1	ADP + phosphate + dicyanocobinamide[side 2] + [cobalamin-binding protein][side 1]	-	?
ATP + H2O + dicyanocobinamide-[cobalamin-binding protein][side 1]	-	Vibrio cholerae serotype O1 Classical Ogawa 395	ADP + phosphate + dicyanocobinamide[side 2] + [cobalamin-binding protein][side 1]	-	?
ATP + H2O + dicyanocobinamide-[cobalamin-binding protein][side 1]	-	Vibrio cholerae serotype O1 ATCC 39541	ADP + phosphate + dicyanocobinamide[side 2] + [cobalamin-binding protein][side 1]	-	?
ATP + H2O + dicyanocobinamide-[cobalamin-binding protein][side 1]	-	Vibrio cholerae serotype O1 O395	ADP + phosphate + dicyanocobinamide[side 2] + [cobalamin-binding protein][side 1]	-	?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]	-	Vibrio cholerae serotype O1	ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]	-	?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]	-	Vibrio cholerae serotype O1 Classical Ogawa 395	ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]	-	?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]	-	Vibrio cholerae serotype O1 ATCC 39541	ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]	-	?
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]	-	Vibrio cholerae serotype O1 O395	ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]	-	?
additional information	VcBtuF, the periplasmic substrate binding protein (PBP) of putative ABC transporter BtuF-CD of Vibrio cholerae O395, binds cyanocobalamin and dicyanocobinamide with micromolar dissociation constants (Kd). Productive internalization of these nutrients	Vibrio cholerae serotype O1	?	-	?
additional information	VcBtuF shows a distinctive phenomenon of heme binding with comparable affinity to vitamin B12	Vibrio cholerae serotype O1	?	-	?
additional information	VcBtuF, the periplasmic substrate binding protein (PBP) of putative ABC transporter BtuF-CD of Vibrio cholerae O395, binds cyanocobalamin and dicyanocobinamide with micromolar dissociation constants (Kd). Productive internalization of these nutrients	Vibrio cholerae serotype O1 Classical Ogawa 395	?	-	?
additional information	VcBtuF shows a distinctive phenomenon of heme binding with comparable affinity to vitamin B12	Vibrio cholerae serotype O1 Classical Ogawa 395	?	-	?
additional information	VcBtuF, the periplasmic substrate binding protein (PBP) of putative ABC transporter BtuF-CD of Vibrio cholerae O395, binds cyanocobalamin and dicyanocobinamide with micromolar dissociation constants (Kd). Productive internalization of these nutrients	Vibrio cholerae serotype O1 ATCC 39541	?	-	?
additional information	VcBtuF shows a distinctive phenomenon of heme binding with comparable affinity to vitamin B12	Vibrio cholerae serotype O1 ATCC 39541	?	-	?
additional information	VcBtuF, the periplasmic substrate binding protein (PBP) of putative ABC transporter BtuF-CD of Vibrio cholerae O395, binds cyanocobalamin and dicyanocobinamide with micromolar dissociation constants (Kd). Productive internalization of these nutrients	Vibrio cholerae serotype O1 O395	?	-	?
additional information	VcBtuF shows a distinctive phenomenon of heme binding with comparable affinity to vitamin B12	Vibrio cholerae serotype O1 O395	?	-	?

Synonyms

Synonyms	Comment	Organism
BtuF-CD	-	Vibrio cholerae serotype O1

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
20	-	BtuF binding assay at	Vibrio cholerae serotype O1

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	BtuF binding assay at	Vibrio cholerae serotype O1

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Vibrio cholerae serotype O1

General Information

General Information	Comment	Organism
additional information	molecular dynamics simulation of structure of the cobalamin-binding protein BtuF compared to Escherichia coli BtuF structure	Vibrio cholerae serotype O1