Literature summary for 7.6.2.8 extracted from

Okamoto, T.; Kawaguchi, K.; Watanabe, S.; Agustina, R.; Ikejima, T.; Ikeda, K.; Nakano, M.; Morita, M.; Imanaka, T.
Characterization of human ATP-binding cassette protein subfamily D reconstituted into proteoliposomes (2018), Biochem. Biophys. Res. Commun., 496, 1122-1127 .

Search for more literature for 7.6.2.8

Cloned(Commentary)

Cloned (Comment)	Organism
gene ABCD4, recombinant expression of His-tagged protein ABCD4 in Pichia pastoris strain SMD1168	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
AlF3	inhibition of the ATPase activity of liposome-reconstituted His-hABCD4	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
lysosomal membrane	ABCD4 dimer makes a complex with lysosomal membrane protein LMBD1, an adopter protein, and targets to lysosome	Homo sapiens	5765	-
additional information	ABCD4 barely interacts with the peroxisomal biogenesis factor Pex19p, because of its lack of NH2-terminal hydrophobic motif. As a result, the transmembrane domain (TMD) 1 of ABCD4 is recognized by certain signal recognition particles and integrated into the ER membrane. ABCD4 dimer then makes a complex with lysosomal membrane protein LMBD1, an adopter protein, and targets to lysosome	Homo sapiens	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]	Homo sapiens	-	ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	O14678	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged protein ABCD4 from Pichia pastoris strain SMD1168 by detergent solubilization and nickel affinity chromatography, evaluation of diverse detergents	Homo sapiens

Renatured (Commentary)

Renatured (Comment)	Organism
recombinant His-tagged protein ABCD4 protein solubilization and reconstitution in proteoliposomes	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1]	-	Homo sapiens	ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1]	-	?
additional information	the enzyme possesses equal levels of acyl-CoA thioesterase activity, ATPase and thioesterase activities of liposome-reconstituted human ABCD4, overview	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
ABCD4	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.6	-	assay at	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Homo sapiens

General Information

General Information	Comment	Organism
malfunction	ABCD4 dysfunction results in a failure of lysosomal vitamin B12 release	Homo sapiens
physiological function	ABCD4 is located on lysosomal membrane and is involved in the transport of vitamin B12 from lysosomes to the cytosol	Homo sapiens

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Release 2023.1 (January 2023)