Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

6.3.4.15: biotin-[biotin carboxyl-carrier protein] ligase

This is an abbreviated version!
For detailed information about biotin-[biotin carboxyl-carrier protein] ligase, go to the full flat file.

Word Map on EC 6.3.4.15

Reaction

ATP
+
biotin
 +
[biotin carboxyl-carrier protein]-L-lysine
=
AMP
 +
diphosphate
 +
[biotin carboxyl-carrier protein]-N6-biotinyl-L-lysine

Synonyms

Acetyl CoA holocarboxylase synthetase, Acetyl coenzyme A holocarboxylase synthetase, bacterial BirA biotin ligase, biotin acetyl-CoA carboxylase ligase, Biotin holoenzyme synthetase, biotin ligase, biotin protein ligase, Biotin--protein ligase, biotin-protein ligase, Biotin-[acetyl coenzyme A carboxylase] synthetase, Biotin-[acetyl-CoA carboxylase] synthetase, Biotin:apocarboxylase ligase, BirA, BirA protein, BPL, group I biotin protein ligase, HCS, Holocarboxylase synthetase, holocarboxylase synthetase 1, More, STK_15250, Synthetase, biotin-[acetyl coenzyme A carboxylase], yBL

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.4 Other carbon-nitrogen ligases
                6.3.4.15 biotin-[biotin carboxyl-carrier protein] ligase

Purification

Purification on EC 6.3.4.15 - biotin-[biotin carboxyl-carrier protein] ligase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
HisTrap column chromatography, Ni-NTA resin column chromatography, and Superdex 75 gel filtration
-
mutants K277E, R317E, and K321E
-
Ni-NTA column chromatography, and Superdex 200 gel filtration
-
recombinant C-terminally Strep-tagged BirA from Escherichia coli
recombinant enzyme comprising residues 1-125 from Escherichia coli strain BL21
-
recombinant enzyme from Escherichia coli
recombinant enzyme from Escherichia coli strain BL21 in a chromatographic procedure comprising several steps
-
recombinant enzyme from Escherichia coli, the human enzyme degrades significantly during isolation
-
recombinant enzyme from Saccharomyces cerevisiae, immobilization of the recombinant enzyme
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration
-
recombinant His-tagged wild-type and mutant enzymes from strain JM109 by nickel affinity chromatography
-
recombinant His6-tagged enzyme from strain BL21(DE3) by nickel affinity chromatography
-
recombinant His9-tagged BPL from Saccharomyces cerevisiae strain w303 by nickel affinity and cation exchange chromatography
-
recombinant human HCS from Hi5 insect cells by nickel affinity chromatography, anion exchange chromatography, and gel filtration
-
recombinant protein
Super Q Toyopearl 650M column chromatography, Bio-Scale CHT-20-I column chromatography, and HiLoad 16/60 Superdex 200 gel filtration
-