Any feedback?
Literature summary for 6.3.4.15 extracted from
- Substrate specificity of archaeon Sulfolobus tokodaii biotin protein ligase (2006), Biochem. Biophys. Res. Commun., 344, 155-159.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21(DE3) | Sulfurisphaera tokodaii |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A43G | catalytic efficiency is 2fold lower than catalytic efficiency of wild-type enzyme | Sulfurisphaera tokodaii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Sulfurisphaera tokodaii |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000026 | - |
biotin | pH 8.0, 37°C, mutant enzyme A43G | Sulfurisphaera tokodaii | |
| 0.00044 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine | pH 8.0, 70°C, wild type enzyme | Sulfurisphaera tokodaii | |
| 0.00058 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine | pH 8.0, 70°C, mutant enzyme A43G | Sulfurisphaera tokodaii | |
| 0.018 | - |
ATP | pH 8.0, 37°C, mutant enzyme A43G | Sulfurisphaera tokodaii | |
| 0.063 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine | pH 8.0, 70°C, mutant enzyme A43G | Sulfurisphaera tokodaii | |
| 0.101 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine | pH 8.0, 70°C, wild type enzyme | Sulfurisphaera tokodaii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sulfurisphaera tokodaii | F9VNG7 | - |
- |
| Sulfurisphaera tokodaii 7 | F9VNG7 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sulfurisphaera tokodaii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + biotin + [Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine | the enzyme from Sulfolobus tokodaii cannot use wild-type biotin carboxyl carrier protein from Escherichia coli as a substrate | Sulfurisphaera tokodaii | AMP + diphosphate + [Sulfolobus tokodaii biotin-carboxyl-carrier-protein mutant S136M]-biotinyllysine | - |
? | |
| ATP + biotin + [Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine | the enzyme from Sulfolobus tokodaii cannot use wild-type biotin carboxyl carrier protein from Escherichia coli as a substrate | Sulfurisphaera tokodaii 7 | AMP + diphosphate + [Sulfolobus tokodaii biotin-carboxyl-carrier-protein mutant S136M]-biotinyllysine | - |
? | |
| ATP + biotin + [Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine | the enzyme from Sulfolobus tokodaii cannot use wild-type biotin carboxyl carrier protein from Escherichia coli as a substrate | Sulfurisphaera tokodaii | AMP + diphosphate + [Sulfolobus tokodaii biotin-carboxyl-carrier-protein]-biotinyllysine | - |
? | |
| ATP + biotin + [Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine | the enzyme from Sulfolobus tokodaii cannot use wild-type biotin carboxyl carrier protein from Escherichia coli as a substrate | Sulfurisphaera tokodaii 7 | AMP + diphosphate + [Sulfolobus tokodaii biotin-carboxyl-carrier-protein]-biotinyllysine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | biotin-protein-ligase/holo-biotin-carboxyl-carrier-protein complex | Sulfurisphaera tokodaii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| STK_15250 | locus name | Sulfurisphaera tokodaii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
no denaturation | Sulfurisphaera tokodaii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0022 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine | pH 8.0, 70°C, mutant enzyme A43G | Sulfurisphaera tokodaii | |
| 0.004 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine | pH 8.0, 70°C, wild type enzyme | Sulfurisphaera tokodaii | |
| 0.082 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine | pH 8.0, 70°C, mutant enzyme A43G | Sulfurisphaera tokodaii | |
| 0.165 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine | pH 8.0, 70°C, wild type enzyme | Sulfurisphaera tokodaii |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.36 | - |
ATP | pH 8.0, 37°C, mutant enzyme A43G | Sulfurisphaera tokodaii | |
| 0.62 | - |
ATP | pH 8.0, 37°C, wild type enzyme | Sulfurisphaera tokodaii | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.3 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine | pH 8.0, 70°C, mutant enzyme A43G | Sulfurisphaera tokodaii | |
| 1.6 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine | pH 8.0, 70°C, wild type enzyme | Sulfurisphaera tokodaii | |
| 3.8 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine | pH 8.0, 70°C, mutant enzyme A43G | Sulfurisphaera tokodaii | |
| 9.1 | - |
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine | pH 8.0, 70°C,wild type enzyme | Sulfurisphaera tokodaii |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
